CAPNS1 Antibody

CAPNS1 Antibody

CATALOGUE NUMBER

ANT-587

SYNONYMS

Calpain Small Subunit 1, CAPN4, Calcium-Activated Neutral Proteinase Small Subunit, Calcium-Dependent Protease Small Subunit 1, Calpain Regulatory Subunit, CANP Small Subunit, CDPS, CSS1, Calcium-Dependent Protease Small Subunit, Calcium-Dependent Protease Small Subunit, Calpain 4 Small Subunit (30K), Calpain Small Polypeptide, Calpain Small Subunit 1, CALPAIN4, CANPS, CAPNS, CANP, CAPNS1.

TYPE

Mouse Anti Human Monoclonal.

INTRODUCTION

Calpain, Small Subunit 1 (CAPNS1) belongs to the calpain small subunit family. Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases, widely distributed in mammalian cells. Calpain families are implicated in neurodegenerative processes, considering that their activation can be triggered by calcium influx and oxidative stress. Calpains function as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by the CAPNS1 gene. The CAPNS1 protein is vital for the stability and function of both calpain heterodimers, whose proteolytic activities influence numerous cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy.

CLONE

PAT1D11AT.

PHYSICAL APPEARANCE

Sterile filtered colorless solution.

IMMUNOGEN

Anti-human CAPNS1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CAPNS1 protein 84-268 amino acids purified from E. coli.

IG SUBCLASS

Mouse IgG₁ heavy chain and k light chain.

PURIFICATION METHOD

CAPNS1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.

FORMULATION

1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.

STORAGE PROCEDURES

For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. 
Prevent freeze thaw cycles.

STABILITY / SHELF LIFE

12 months at -20°C. 1 month at 4°C.

APPLICATIONS

CAPNS1 antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

USAGE

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.