P35GC-1.0-14-C

Use of Lectins for Characterization of O-Linked Glycans of Herpes Simplex Virus Glycoproteins

The existence of O -linked glycans in viral glycoproteins was described in the early 1980s for enveloped viruses such as herpes simplex virus type 1 (HSV-1), vaccinia virus, and mouse hepatitis virus (1 -4 ). Glycoprotein C

Crystallization of Kinesin Family Motor Proteins

by a short coiled-coil; Kozielski et al.,1997); the Kinesin-14 (formerly C-terminal motor)proteins Drosophila Ncd in its monomeric and dimeric forms (Sablin & Fletterick,1995,Sablin et al.,1996,1998; Kozielski et al.,1997; Yun et al.,2003),yeast KAR3 (monomer

Replication timing by comparative hybridization

the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml