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- 文献和实验
- 技术资料
- 抗体名:
Goat anti-Rabbit IgG (H+L) Secondary Antibody, PE
- 靶点:
Rabbit IgG (H+L)
- 应用范围:
Immunocytochemistry, Immunofluorescence
- 宿主:
Goat
- 适应物种:
Rabbit
- 克隆性:
Polyclonal
- 保存条件:
4° C
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
Gamma Immunoglobins Heavy and Light chains
- 规格:
500 µL
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文献和实验secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
T-cell dependent antigen specific in situ staining (Pe)
HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’. Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide
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