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- 文献和实验
- 技术资料
- 抗体名:
CD4 Monoclonal Antibody (RM4-5), PE
- 靶点:
CD4
- 应用范围:
Flow Cytometry
- 宿主:
Rat
- 适应物种:
Mouse
- 克隆性:
Monoclonal
- 保存条件:
4° C
- 形态:
Liquid
- 亚型:
IgG2a
- 免疫原:
Mouse CD4
- 规格:
500 µL
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文献和实验Western Blotting with Horseradish Pe
ELECTROPHORESIS Guidelines for choosing the percent gel to be used for certain molecular weight proteins (based on 37:1 acrylamide: bis acrylamide ratio) 4-5% gels: > 250 kDa 7.5% gels: 250-120 kDa 10% gels: 120-40 kDa 13% gels: 40-15 kDa 15% gels: Gel
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Western Blotting with Monoclonal Ant
at 37˚C or one hour at room temperature. Substrate Incubation 1.Decant the secondary antibody solution, add wash buffer (10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20), and wash for 30 minutes with agitation, changing the wash buffer every 3-5 minutes
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