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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20 C
- 英文名:
Mouse Serum Anti-OVA IgE Antibody Kit
- 库存:
1
- 供应商:
北京博蕾德生物科技有限公司
- 规格:
1 kit
小鼠抗卵清蛋白IgE抗体检测试剂盒
Cat#3010
| 产品名称 | 小鼠抗卵清蛋白IgE抗体检测试剂盒 |
| 形式 | 96孔可拆版 |
| 检测方法 | 夹心ELISA |
| 检测时间 | 5.5小时 |
| 检测范围 | 0.4-25ng/ml |
| 检测样本数 | 40例(复孔) |
| 样本类型 | 血清&血浆 |
| 样本稀释比 | ≥1:10 |
| 显色底物 | TMB(450nm读取OD) |
| 保存温度 | -20℃ |
| 验证数据 | 批间(7.1%)/批内(5.3-12.8%)/回收实验(82-92%) |
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文献和实验1. Morokawa T. et al. Differential susceptibility of C57BL/6 and DBA/2 mice to ovalbumin-induced pulmonary eosinophil regulated by Th1/Th2 type cytokines. Immunol. Letter 70: 127-134 (1999).
2. Oshiba A. et al. Passive transfer of immediate-hypersensitivity and airway hyperresponsiveness by allergen-specific immunoglobulin IgE and IgG1 in mice. J. Clin. Invest. 97: 1398-1408 (1996).
3. Hamelmann E. et al. Role of IgE in the development of allergic airway inflammation and airway hyperresponsiveness-a marine model.Allergy. 54: 297-305 (1999).
4. Taube C. et al. Mast cells, FceRI, and IL-13 are required for development of airway hyperresponsiveness after aerosolized allergen exposure in the absence of adjuvant. J. Immunol. 172: 6398-6406 (2004).
Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE
to anaphylaxis. Here, we describe the history behind the IgE discovery and the protocol for purifying IgE and anti-IgE in the mouse. This is followed by our ELISA protocol for mouse IgE detection.
Aspects of CNS Lupus: Mouse Models of Anti-NMDA Receptor Antibody Mediated Reactivity
concepts of these models and provide protocols for the following: (1) the induction of anti-dsDNA, anti-NMDAR antibodies, (2) testing serum antibody titer by ELISA, (3) breaching blood brain barrier (BBB) integrity with LPS and epinephrine, (4) passive
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
/) 96‐well plate (8 well removable strips) coated with polyclonal rabbit anti‐PINP antibody (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/) Precision









