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- 详细信息
- 文献和实验
- 技术资料
- 库存:
200
- 供应商:
上海攸碧艾
- 英文名:
DMEM (Low Glucose)
- 规格:
500ml
产品简介:
Dulbecco 的改良 Eagle 培养基—DMEM(Dulbecco's Modified Eagle Medium)是一种广泛使用的基础培养基,适用于多种哺乳动物细胞培养,包括原代成纤维细胞,神经元,神经胶质 细胞,HUVEC 和平滑肌细胞,以及 HeLa , 293 ,Cos-7 和 PC-12 等细胞系。
DMEM 是在 MEM 培养基的基础上研制的,与 MEM 培养基相比,氨基酸的含量增加了 2 倍,维生素增加了 4 倍,同时还增加了非必须氨基酸、微量铁离子以及bingtong酸钠。
DMEM 培养基最初设计为葡萄糖含量 1000mg/L 的低糖型,后来又发展出葡萄糖含量为 4500mg/L 的高糖型,现已广泛应用于各种细胞的培养。DMEM 低糖培养基更适合代谢作 用较慢、依赖性贴壁细胞的培养。
L-谷氨酰胺是细胞培养液体环境中所必需的一种营养成分,但其在水溶液中不稳定, 易降解。bingtong酸钠可以作为细胞培养中的替代碳源,在葡萄糖不足的情况下,细胞也可以 代谢bingtong酸钠。
本产品含有:D-葡萄糖(1000mg/L)、酚红、L-谷氨酰胺、bingtong酸钠。
不含有:HEPES、双抗。
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文献和实验Glucose Dehydrogenase for the Regeneration of NADPH and NADH
Glucose dehydrogenases (GDHs) occur in several organisms such as Bacillus megaterium and Bacillus subtilis. They accept both NAD+ and NADP+ as cofactor and can be used for the regeneration of NADH and NADPH. In order to demonstrate
The Effect of Caffeine on the Heart Rates of Three-Day Old Chick Embryos
due to the presence of glucose as an energy source in the DMEM. Also consistent with our expectations, dilute caffeine solutions (0.1 mg/ml and 0.2 mg/ml) did further increase the heart rates relative to the base rate (see table 3). However, we did not expect
The overexpression of toxic recombinant proteins is often problematic, leading to either low production levels or inclusion bodies. Streptavidin is no exception and thus the highest production level reported to date for streptavidin is 70 mg











