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大量
- 英文名：
Lyra Influenza A+B Assay
- 保质期：
详见说明书
- 供应商：
钰博生物
- 保存条件：
详见官方网站
The Lyra Influenza A+B Assay is a multiplex real-time RT-PCR assay for the qualitative detection and identification of viral nucleic acids extracted from nasal swabs and nasopharyngeal swabs specimens. The real-time influenza test has been shown to detect HIN1, H3N2 and H7N9 subtypes but does not differentiate the subtypes from Influenza A.
A multiplex RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for each of the target viruses present in the sample. Identification of influenza A occurs by the use of target specific primers and a fluorescent-labeled probe that hybridizes to a conserved region within the matrix protein gene. Identification of influenza B occurs by the use of target specific primers and fluorescent-labeled probes that hybridize to a conserved influenza B sequence within the neuraminidase gene.
The Lyra Influenza A+B Assay has a one-step reagent set up. Test results are available in less than 75 minutes after extraction. Lyra Influenza A+B RT-PCR is available for use on the Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument, QuantStudioTM Dx Real-Time PCR Instrument and Cepheid®SmartCycler® II.
The Lyra Influenza A+B Assay is one in a growing menu of assays and is intended to aid in the differential diagnosis of influenza A and/or influenza B viral infections in humans.
Features & Benefits
| Feature | Benefit |
|---|---|
| One-step reagent set-up | Rehydration solution is simply added to the lyophilized Master Mix |
| Speed | Results in less than 75 minutes after extraction |
| Easy training | Simplified and uniform workflow with standard pipetting volumes |
| Refrigerated storage | No freezer needed. 2°C to 8°C storage |
| Long shelf life | 2 years from date of manufacture |
| Room temperature set up | No ice or cooling block required |
| Flexible 96-test format | Kit includes vials of 12x8 Master Mix, Rehydration Solution and liquid Process Controls |
| Multi-well multiplex panels | Standardized thermocycling conditions for the respiratory assays allow for multiple analytes to be analyzed on the same plate from the same extracted specimen, effectively providing a customizable panel of results |
Product Specifications
| Sample type | Nasal swab, Nasopharyngeal swab |
| Time to results | Less than 75 minutes after extraction |
| Reagent storage conditions | 2°C to 8°C |
| Controls storage conditions | 2°C to 8°C |
| Positive Percent Agreement* | A: 100%; B: 98.4% |
| Negative Percent Agreement* | A: 98.7%; B: 95.5% |
| LOD | 2 to 100 TCID50/mL |
| Sample volume needed for extraction | 180 µL |
| Purified nucleic acid volume needed to testing | 5 µL |
| Shelf life | 24 months |
*Refer to Package Insert for additional performance claims.
Ordering Information
| Catalog Number | Description | Kit Size / Case Size |
|---|---|---|
| M100 | Assay Kit |
96 tests |
| M106 | Quidel Molecular Influenza A+B Control Set |
10 reactions |
Product Documentation
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