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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Rat MIP-3 alpha/CCL20 ELISA Kit
- 保质期：
1年
- 供应商：
上海钰博生物科技有限公司
- 保存条件：
-2-8℃
- 规格：
盒
Rat MIP-3 alpha/CCL20 ELISA Kit
Product Description
Rat MIP-3 alpha/CCL20 ELISA Kit - 1 Kit (96 Wells)
Our Rat MIP-3 alpha/CCL20 ELISA Kit is a sandwich ELISA intended for the quantitative determination of rat MIP-3 alpha in cell culture supernates, serum and plasma (heparin, EDTA).Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4() and CD8() T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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