pU2X2

pU2X2

质粒名称: pU2X2
其他名称: 
质粒大小（bp）: 8100
抗性: 
质粒类型: Unspecified
是否病毒: Unspecified 
启动子: 
稳转或瞬转: Unspecified 
组成型或诱导性: Unspecified 
表达水平: 
蛋白标签: 
测序引物: 
GenBank号: 
其他属性:

Notes:

Shuttle expression vector using Ustilago maydis promoters gap (glyceraldehyde phosphate dehydrogenase) or hsp70 to drive expression of the cloned insert in the BamHI or KpnI cloning site respectively. Constructed from pUXV3 (ATCC 77465) by inserting a 2.1 kb hsp70 promoter and termination cassette into the KpnI site, creating a unique KpnI cloning site between the promoter and terminator. Differs from pU2X1 (ATCC 77466) only in the orientation of the hsp70 promoter-terminator cassette. The order of the major features in the plasmid is: Ustilago maydis ARS – ampR – pMB1 ori – 5′ lacZ fragment – EcoRI – SacI <- hsp70 terminator – KpnI <- hsp70 promoter – gap promoter -> BamHI <- cbxR – 3′ lacZ fragment. [2] The sequence conferring resistance to carboxin (cbxR) is a mutation of an iron-sulfur protein subunit of the Ustilago maydis succinate dehydrogenase gene. [3] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: J.A.Bruenn Hosts: Ustilago maydis, E.coli.