pRS306

pRS306

质粒名称: pRS306
其他名称: 
质粒大小（bp）: 4373
抗性: Ampicillin 
质粒类型: Yeast Expression
是否病毒: Unspecified 
启动子: 
稳转或瞬转: Unspecified 
组成型或诱导性: Unspecified 
表达水平: 
蛋白标签: 
测序引物: 
GenBank号: U03438 
其他属性: One of a series of pBluescript-based integrating vectors (ATCC 77138-77141) differing in the yeast selectable marker gene. The order of the major features in this plasmid is: URA3 – f1 ori (NaeI) – T7 promoter – lacZ'/MCS – T3 promoter – pMB1 ori – bla. YI-type integrating shuttle vector permitting visual detection of recombinants and production of ssDNA in E.coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment (1.112 kb) containing the URA3 gene was inserted into the NdeI site of pRSS56. All ends were blunted. Restriction digests of the clone give the following sizes (kb): PstI–2.7, 1.65; PvuI–3.9, 0.40; EcoRI–4.4; HindIII–4.4. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416307 Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH499, Saccharomyces cerevisiae YPH500, Saccharomyces cerevisiae YPH501. Related vectors: pRS304, pRS305, pRS303.
质粒图谱:

 

 

质粒序列:

1	tcgcgcgttt	cggtgatgac	ggtgaaaacc	tctgacacat	gcagctcccg	50
51	gagacggtca	cagcttgtct	gtaagcggat	gccgggagca	gacaagcccg	100
101	tcagggcgcg	tcagcgggtg	ttggcgggtg	tcggggctgg	cttaactatg	150
151	cggcatcaga	gcagattgta	ctgagagtgc	accacgcttt	tcaattcaat	200
201	tcatcatttt	ttttttattc	ttttttttga	tttcggtttc	tttgaaattt	250
251	ttttgattcg	gtaatctccg	aacagaagga	agaacgaagg	aaggagcaca	300
301	gacttagatt	ggtatatata	cgcatatgta	gtgttgaaga	aacatgaaat	350
351	tgcccagtat	tcttaaccca	actgcacaga	acaaaaacct	gcaggaaacg	400
401	aagataaatc	atgtcgaaag	ctacatataa	ggaacgtgct	gctactcatc	450
451	ctagtcctgt	tgctgccaag	ctatttaata	tcatgcacga	aaagcaaaca	500
501	aacttgtgtg	cttcattgga	tgttcgtacc	accaaggaat	tactggagtt	550
551	agttgaagca	ttaggtccca	aaatttgttt	actaaaaaca	catgtggata	600
601	tcttgactga	tttttccatg	gagggcacag	ttaagccgct	aaaggcatta	650
651	tccgccaagt	acaatttttt	actcttcgaa	gacagaaaat	ttgctgacat	700
701	tggtaataca	gtcaaattgc	agtactctgc	gggtgtatac	agaatagcag	750
751	aatgggcaga	cattacgaat	gcacacggtg	tggtgggccc	aggtattgtt	800
801	agcggtttga	agcaggcggc	agaagaagta	acaaaggaac	ctagaggcct	850
851	tttgatgtta	gcagaattgt	catgcaaggg	ctccctatct	actggagaat	900
901	atactaaggg	tactgttgac	attgcgaaga	gcgacaaaga	ttttgttatc	950
951	ggctttattg	ctcaaagaga	catgggtgga	agagatgaag	gttacgattg	1000
1001	gttgattatg	acacccggtg	tgggtttaga	tgacaaggga	gacgcattgg	1050
1051	gtcaacagta	tagaaccgtg	gatgatgtgg	tctctacagg	atctgacatt	1100
1101	attattgttg	gaagaggact	atttgcaaag	ggaagggatg	ctaaggtaga	1150
1151	gggtgaacgt	tacagaaaag	caggctggga	agcatatttg	agaagatgcg	1200
1201	gccagcaaaa	ctaaaaaact	gtattataag	taaatgcatg	tatactaaac	1250
1251	tcacaaatta	gagcttcaat	ttaattatat	cagttattac	cctgcggtgt	1300
1301	gaaataccgc	acagatgcgt	aaggagaaaa	taccgcatca	ggaaattgta	1350
1351	aacgttaata	ttttgttaaa	attcgcgtta	aatttttgtt	aaatcagctc	1400
1401	attttttaac	caataggccg	aaatcggcaa	aatcccttat	aaatcaaaag	1450
1451	aatagaccga	gatagggttg	agtgttgttc	cagtttggaa	caagagtcca	1500
1501	ctattaaaga	acgtggactc	caacgtcaaa	gggcgaaaaa	ccgtctatca	1550
1551	gggcgatggc	ccactacgtg	aaccatcacc	ctaatcaagt	tttttggggt	1600
1601	cgaggtgccg	taaagcacta	aatcggaacc	ctaaagggag	cccccgattt	1650
1651	agagcttgac	ggggaaagcc	ggcgaacgtg	gcgagaaagg	aagggaagaa	1700
1701	agcgaaagga	gcgggcgcta	gggcgctggc	aagtgtagcg	gtcacgctgc	1750
1751	gcgtaaccac	cacacccgcc	gcgcttaatg	cgccgctaca	gggcgcgtcg	1800
1801	cgccattcgc	cattcaggct	gcgcaactgt	tgggaagggc	gatcggtgcg	1850
1851	ggcctcttcg	ctattacgcc	agctggcgaa	ggggggatgt	gctgcaaggc	1900
1901	gattaagttg	ggtaacgcca	gggttttccc	agtcacgacg	ttgtaaaacg	1950
1951	acggccagtg	aattgtaata	cgactcacta	tagggcgaat	tggagctcca	2000
2001	ccgcggtggc	ggccgctcta	gaactagtgg	atcccccggg	ctgcaggaat	2050
2051	tcgatatcaa	gcttatcgat	accgtcgacc	tcgagggggg	gcccggtacc	2100
2101	cagcttttgt	tccctttagt	gagggttaat	tccgagcttg	gcgtaatcat	2150
2151	ggtcatagct	gtttcctgtg	tgaaattgtt	atccgctcac	aattccacac	2200
2201	aacataggag	ccggaagcat	aaagtgtaaa	gcctggggtg	cctaatgagt	2250
2251	gaggtaactc	acattaattg	cgttgcgctc	actgcccgct	ttccagtcgg	2300
2301	gaaacctgtc	gtgccagctg	cattaatgaa	tcggccaacg	cgcggggaga	2350
2351	ggcggtttgc	gtattgggcg	ctcttccgct	tcctcgctca	ctgactcgct	2400
2401	gcgctcggtc	gttcggctgc	ggcgagcggt	atcagctcac	tcaaaggcgg	2450
2451	taatacggtt	atccacagaa	tcaggggata	acgcaggaaa	gaacatgtga	2500
2501	gcaaaaggcc	agcaaaaggc	caggaaccgt	aaaaaggccg	cgttgctggc	2550
2551	gtttttccat	aggctcggcc	cccctgacga	gcatcacaaa	aatcgacgct	2600
2601	caagtcagag	gtggcgaaac	ccgacaggac	tataaagata	ccaggcgttc	2650
2651	ccccctggaa	gctccctcgt	gcgctctcct	gttccgaccc	tgccgcttac	2700
2701	cggatacctg	tccgcctttc	tcccttcggg	aagcgtggcg	ctttctcaat	2750
2751	gctcacgctg	taggtatctc	agttcggtgt	aggtcgttcg	ctccaagctg	2800
2801	ggctgtgtgc	acgaaccccc	cgttcagccc	gaccgctgcg	ccttatccgg	2850
2851	taactatcgt	cttgagtcca	acccggtaag	acacgactta	tcgccactgg	2900
2901	cagcagccac	tggtaacagg	attagcagag	cgaggtatgt	aggcggtgct	2950
2951	acagagttct	tgaagtggtg	gcctaactac	ggctacacta	gaaggacagt	3000
3001	atttggtatc	tgcgctctgc	tgaagccagt	taccttcgga	aaaagagttg	3050
3051	gtagctcttg	atccggcaaa	caaaccaccg	ctggtagcgg	tggttttttt	3100
3101	gtttgcaagc	agcagattac	gcgcagaaaa	aaaggatctc	aagaagatcc	3150
3151	tttgatcttt	tctacggggt	ctgacgctca	gtggaacgaa	aactcacgtt	3200
3201	aagggatttt	ggtcatgaga	ttatcaaaaa	ggatcttcac	ctagatcctt	3250
3251	ttaaattaaa	aatgaagttt	taaatcaatc	taaagtatat	atgagtaaac	3300
3301	ttggtctgac	agttaccaat	gcttaatcag	tgaggcacct	atctcagcga	3350
3351	tctgtctatt	tcgttcatcc	atagttgcct	gactgcccgt	cgtgtagata	3400
3401	actacgatac	gggagggctt	accatctggc	cccagtgctg	caatgatacc	3450
3451	gcgagaccca	cgctcaccgg	ctccagattt	atcagcaata	aaccagccag	3500
3501	ccggaagggc	cgagcgcaga	agtggtcctg	caactttatc	cgcctccatc	3550
3551	cagtctatta	attgttgccg	ggaagctaga	gtaagtagtt	cgccagttaa	3600
3601	tagtttgcgc	aacgttgttg	ccattgctac	aggcatcgtg	gtgtcacgct	3650
3651	cgtcgtttgg	tatggcttca	ttcagctccg	gttcccaacg	atcaaggcga	3700
3701	gttacatgat	cccccatgtt	gtgaaaaaaa	gcggttagct	ccttcggtcc	3750
3751	tccgatcgtt	gtcagaagta	agttggccgc	agtgttatca	ctcatggtta	3800
3801	tggcagcact	gcataattct	cttactgtca	tgccatccgt	aagatgcttt	3850
3851	tctgtgactg	gtgagtactc	aaccaagtca	ttctgagaat	agtgtatgcg	3900
3901	gcgaccgagt	tgctcttgcc	cggcgtcaat	acgggataat	accgcgccac	3950
3951	atagcagaac	tttaaaagtg	ctcatcattg	gaaaacgttc	ttcggggcga	4000
4001	aaactctcaa	ggatcttacc	gctgttgaga	tccagttcga	tgtaacccac	4050
4051	tcgtgcaccc	aactgatctt	cagcatcttt	tactttcacc	agcgtttctg	4100
4101	ggtgagcaaa	aacaggaagg	caaaatgccg	caaaaaaggg	aataagggcg	4150
4151	acacggaaat	gttgaatact	catactcttc	ctttttcaat	attattgaag	4200
4201	catttatcag	ggttattgtc	tcatgagcgg	atacatattt	gaatgtattt	4250
4251	agaaaaataa	acaaataggg	gttccgcgca	catttccccg	aaaagtgcca	4300
4301	cctgacgtct	aagaaaccat	tattatcatg	acattaacct	ataaaaatag	4350
4351	gcgtatcacg	aggccctttc	gtc	4373