pGEX-1(pGEX1)

pGEX-1(pGEX1)

质粒名称: pGEX-1
其他名称: 
质粒大小（bp）: 4932
抗性: Ampicillin 
质粒类型: Unspecified
是否病毒: Unspecified 
启动子: 
稳转或瞬转: Unspecified 
组成型或诱导性: Unspecified 
表达水平: 
蛋白标签: 
测序引物: 
GenBank号: M21676, A01437 
其他属性: Expression vector permitting production of a fusion protein. (ATCC staff) The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin. [2] Constructed from pGEX-2T by inserting an oligonucleotide at the EcoRI site which encodes the glycine kinker and additional restriction sites to facilitate cloning in all reading frames. [2] The order of the major features in this plasmid are: Ptac – GST – thrombin cleavage site – BamHI site – SmaI site – glycine kinker – multiple cloning site – ampR – pBR322 ori – lacIq. [2] Restriction digests of the clone give the following sizes (kb): HindIII–5.0; BamHI–5.0; EcoRI–5.0; XbaI–5.0. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 208304 Hosts: E.coli HB101, E.coli, E.coli TG1, E.coli JM101 
质粒图谱:

 

质粒序列:

1	agcttatcga	ctgcacggtg	caccaatgct	tctggcgtca	ggcagccatc	50
51	ggaagctgtg	gtatggctgt	gcaggtcgta	aatcactgca	taattcgtgt	100
101	cgctcaaggc	gcactcccgt	tctggataat	gttttttgcg	ccgacatcat	150
151	aacggttctg	gcaaatattc	tgaaatgagc	tgttgacaat	taatcatcgg	200
201	ctcgtataat	gtgtggaatt	gtgagcggat	aacaatttca	cacaggaaac	250
251	agtattcatg	tcccctatac	taggttattg	gaaaattaag	ggccttgtgc	300
301	aacccactcg	acttcttttg	gaatatcttg	aagaaaaata	tgaagagcat	350
351	ttgtatgagc	gcgatgaagg	tgataaatgg	cgaaacaaaa	agtttgaatt	400
401	gggtttggag	tttcccaatc	ttccttatta	tattgatggt	gatgttaaat	450
451	taacacagtc	tatggccatc	atacgttata	tagctgacaa	gcacaacatg	500
501	ttgggtggtt	gtccaaaaga	gcgtgcagag	atttcaatgc	ttgaaggagc	550
551	ggttttggat	attagatacg	gtgtttcgag	aattgcatat	agtaaagact	600
601	ttgaaactct	caaagttgat	tttcttagca	agctacctga	aatgctgaaa	650
651	atgttcgaag	atcgtttatg	tcataaaaca	tatttaaatg	gtgatcatgt	700
701	aacccatcct	gacttcatgt	tgtatgacgc	tcttgatgtt	gttttataca	750
751	tggacccaat	gtgcctggat	gcgttcccaa	aattagtttg	ttttaaaaaa	800
801	cgtattgaag	ctatcccaca	aattgataag	tacttgaaat	ccagcaagta	850
851	tatagcatgg	cctttgcagg	gctggcaagc	cacgtttggt	ggtggcgacc	900
901	atcctccaaa	atcggatccc	cgggaattca	tcgtgactga	ctgacgatct	950
951	gcctcgcgcg	tttcggtgat	gacggtgaaa	acctctgaca	catgcagctc	1000
1001	ccggagacgg	tcacagcttg	tctgtaagcg	gatgccggga	gcagacaagc	1050
1051	ccgtcagggc	gcgtcagcgg	gtgttggcgg	gtgtcggggc	gcagccatga	1100
1101	cccagtcacg	tagcgatagc	ggagtgtata	attcttgaag	acgaaagggc	1150
1151	ctcgtgatac	gcctattttt	ataggttaat	gtcatgataa	taatggtttc	1200
1201	ttagacgtca	ggtggcactt	ttcggggaaa	tgtgcgcgga	acccctattt	1250
1251	gtttattttt	ctaaatacat	tcaaatatgt	atccgctcat	gagacaataa	1300
1301	ccctgataaa	tgcttcaata	atattgaaaa	aggaagagta	tgagtattca	1350
1351	acatttccgt	gtcgccctta	ttcccttttt	tgcggcattt	tgccttcctg	1400
1401	tttttgctca	cccagaaacg	ctggtgaaag	taaaagatgc	tgaagatcag	1450
1451	ttgggtgcac	gagtgggtta	catcgaactg	gatctcaaca	gcggtaagat	1500
1501	ccttgagagt	tttcgccccg	aagaacgttt	tccaatgatg	agcactttta	1550
1551	aagttctgct	atgtggcgcg	gtattatccc	gtgttgacgc	cgggcaagag	1600
1601	caactcggtc	gccgcataca	ctattctcag	aatgacttgg	ttgagtactc	1650
1651	accagtcaca	gaaaagcatc	ttacggatgg	catgacagta	agagaattat	1700
1701	gcagtgctgc	cataaccatg	agtgataaca	ctgcggccaa	cttacttctg	1750
1751	acaacgatcg	gaggaccgaa	ggagctaacc	gcttttttgc	acaacatggg	1800
1801	ggatcatgta	actcgccttg	atcgttggga	accggagctg	aatgaagcca	1850
1851	taccaaacga	cgagcgtgac	accacgatgc	ctgcagcaat	ggcaacaacg	1900
1901	ttgcgcaaac	tattaactgg	cgaactactt	actctagctt	cccggcaaca	1950
1951	attaatagac	tggatggagg	cggataaagt	tgcaggacca	cttctgcgct	2000
2001	cggcccttcc	ggctggctgg	tttattgctg	ataaatctgg	agccggtgag	2050
2051	cgtgggtctc	gcggtatcat	tgcagcactg	gggccagatg	gtaagccctc	2100
2101	ccgtatcgta	gttatctaca	cgacggggag	tcaggcaact	atggatgaac	2150
2151	gaaatagaca	gatcgctgag	ataggtgcct	cactgattaa	gcattggtaa	2200
2201	ctgtcagacc	aagtttactc	atatatactt	tagattgatt	taaaacttca	2250
2251	tttttaattt	aaaaggatct	aggtgaagat	cctttttgat	aatctcatga	2300
2301	ccaaaatccc	ttaacgtgag	ttttcgttcc	actgagcgtc	agaccccgta	2350
2351	gaaaagatca	aaggatcttc	ttgagatcct	ttttttctgc	gcgtaatctg	2400
2401	ctgcttgcaa	acaaaaaaac	caccgctacc	agcggtggtt	tgtttgccgg	2450
2451	atcaagagct	accaactctt	tttccgaagg	taactggctt	cagcagagcg	2500
2501	cagataccaa	atactgtcct	tctagtgtag	ccgtagttag	gccaccactt	2550
2551	caagaactct	gtagcaccgc	ctacatacct	cgctctgcta	atcctgttac	2600
2601	cagtggctgc	tgccagtggc	gataagtcgt	gtcttaccgg	gttggactca	2650
2651	agacgatagt	taccggataa	ggcgcagcgg	tcgggctgaa	cggggggttc	2700
2701	gtgcacacag	cccagcttgg	agcgaacgac	ctacaccgaa	ctgagatacc	2750
2751	tacagcgtga	gctatgagaa	agcgccacgc	ttcccgaagg	gagaaaggcg	2800
2801	gacaggtatc	cggtaagcgg	cagggtcgga	acaggagagc	gcacgaggga	2850
2851	gcttccaggg	ggaaacgcct	ggtatcttta	tagtcctgtc	gggtttcgcc	2900
2901	acctctgact	tgagcgtcga	tttttgtgat	gctcgtcagg	ggggcggagc	2950
2951	ctatggaaaa	acgccagcaa	cgcggccttt	ttacggttcc	tggccttttg	3000
3001	ctggcctttt	gctcacatgt	tctttcctgc	gttatcccct	gattctgtgg	3050
3051	ataaccgtat	taccgccttt	gagtgagctg	ataccgctcg	ccgcagccga	3100
3101	acgaccgagc	gcagcgagtc	agtgagcgag	gaagcggaag	agcgcctgat	3150
3151	gcggtatttt	ctccttacgc	atctgtgcgg	tatttcacac	cgcataaatt	3200
3201	ccgacaccat	cgaatggtgc	aaaacctttc	gcggtatggc	atgatagcgc	3250
3251	ccggaagaga	gtcaattcag	ggtggtgaat	gtgaaaccag	taacgttata	3300
3301	cgatgtcgca	gagtatgccg	gtgtctctta	tcagaccgtt	tcccgcgtgg	3350
3351	tgaaccaggc	cagccacgtt	tctgcgaaaa	cgcgggaaaa	agtggaagcg	3400
3401	gcgatggcgg	agctgaatta	cattcccaac	cgcgtggcac	aacaactggc	3450
3451	gggcaaacag	tcgttgctga	ttggcgttgc	cacctccagt	ctggccctgc	3500
3501	acgcgccgtc	gcaaattgtc	gcggcgatta	aatctcgcgc	cgatcaactg	3550
3551	ggtgccagcg	tggtggtgtc	gatggtagaa	cgaagcggcg	tcgaagcctg	3600
3601	taaagcggcg	gtgcacaatc	ttctcgcgca	acgcgtcagt	gggctgatca	3650
3651	ttaactatcc	gctggatgac	caggatgcca	ttgctgtgga	agctgcctgc	3700
3701	actaatgttc	cggcgttatt	tcttgatgtc	tctgaccaga	cacccatcaa	3750
3751	cagtattatt	ttctcccatg	aagacggtac	gcgactgggc	gtggagcatc	3800
3801	tggtcgcatt	gggtcaccag	caaatcgcgc	tgttagcggg	cccattaagt	3850
3851	tctgtctcgg	cgcgtctgcg	tctggctggc	tggcataaat	atctcactcg	3900
3901	caatcaaatt	cagccgatag	cggaacggga	aggcgactgg	agtgccatgt	3950
3951	ccggttttca	acaaaccatg	caaatgctga	atgagggcat	cgttcccact	4000
4001	gcgatgctgg	ttgccaacga	tcagatggcg	ctgggcgcaa	tgcgcgccat	4050
4051	taccgagtcc	gggctgcgcg	ttggtgcgga	tatctcggta	gtgggatacg	4100
4101	acgataccga	agacagctca	tgttatatcc	cgccgtcaac	caccatcaaa	4150
4151	caggattttc	gcctgctggg	gcaaaccagc	gtggaccgct	tgctgcaact	4200
4201	ctctcagggc	caggcggtga	agggcaatca	gctgttgccc	gtctcactgg	4250
4251	tgaaaagaaa	aaccaccctg	gcgcccaata	cgcaaaccgc	ctctccccgc	4300
4301	gcgttggccg	attcattaat	gcagctggca	cgacaggttt	cccgactgga	4350
4351	aagcgggcag	tgagcgcaac	gcaattaatg	tgagttagct	cactcattag	4400
4401	gcaccccagg	ctttacactt	tatgcttccg	gctcgtatgt	tgtgtggaat	4450
4451	tgtgagcgga	taacaatttc	acacaggaaa	cagctatgac	catgattacg	4500
4501	gattcactgg	ccgtcgtttt	acaacgtcgt	gactgggaaa	accctggcgt	4550
4551	tacccaactt	aatcgccttg	cagcacatcc	ccctttcgcc	agctggcgta	4600
4601	atagcgaaga	ggcccgcacc	gatcgccctt	cccaacagtt	gcgcagcctg	4650
4651	aatggcgaat	ggcgctttgc	ctggtttccg	gcaccagaag	cggtgccgga	4700
4701	aagctggctg	gagtgcgatc	ttcctgaggc	cgatactgtc	gtcgtcccct	4750
4751	caaactggca	gatgcacggt	tacgatgcgc	ccatctacac	caacgtaacc	4800
4801	tatcccatta	cggtcaatcc	gccgtttgtt	cccacggaga	atccgacggg	4850
4851	ttgttactcg	ctcacattta	atgttgatga	aagctggcta	caggaaggcc	4900
4901	agacgcgaat	tatttttgat	ggcgttggaa	tt	4932