pG-KJE8

pG-KJE8
质粒名称: pG-KJE8
其他名称:
质粒大小（bp）: 11.1kb
抗性: chloromycetin (氯霉素)
质粒类型: 大肠杆菌分子伴侣载体
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
质粒图谱:

质粒序列:1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
361 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
481 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaag aatagttacg
541 gcttatgaca tctttgtgga cacatcattc actttttatt cacatccggc cctgaactcg
601 ctaggacttg ccccggtgca ttttttaaat acccgcgaaa aatagagctg atcgtcaaat
661 ccaacattgc gcccaacggt cgctatcggc attcgcgtag tgctaagcag aagtttcgcc
721 tggctgatac gctgatcttc gcgccagctc aatacgctaa tgcctaactg ctggcggaac
781 agatgtgata accgggaggg cgacaggcag acatgctggg cgacgctggc gatatcaaaa
841 tggctgtccg ccagatggtc gctgatatac tggcaggcat cgcgcacacg gctatccatc
901 ggcgggtgca acgactcatt aattaccgcc atacgtctga gcaacaactg ctccagcaga
961 ttgatcgcca gtagctcaga atagcgacct tccccttgcc cggcgctgat gatctgcccg
1021 aacagttcgc tgaaatgcgg ctggcgcgcc tcgtccgggc ggaaaaatcc tgtctgggca
1081 aagattgtcg gccaggtcag ccactcctgc cagtaggcgc gaggccggaa ataaacccac
1141 tggtgatacc actcgctggc gtccggatgc cgtccatagt gatgaatctc gcccggcgga
1201 aacaataata tatcgccagg ccgacagaca aactgctcgc cattattatt aatgacgccc
1261 tctccgcgga tggtcaggtt aagaatatat cccttcatgc ccaacggacg atcgataaaa
1321 aaatccagat atccattcgc ttcaattggc gtcagcccgg cgaccagatg ggcattaaat
1381 gaatatcccg gcaatagcgg atcattttgc gtttcagcca tgatttctct accccccgat
1441 gttcagagaa gaaacaaatt gtccatatcg accaggacga cagagcttcc gtctccgcaa
1501 gactttgcgc ttgatgaaag cacgtatcaa ccccgcttgt gaaaagcgct ttgtaacaaa
1561 agcgtacagt tcaggcgata aaattaagta acagaagtgt ctataactat ggctggaatg
1621 tccacattga atatttgcac agcgtcacac tttgcaaagc attagcattt ttgtccataa
1681 gattagcgga tcctgcctga cggtttttgc cgcgactctc tataatttct ccatacctgt
1741 ttttctggat ggagtaagac catggctatg gattgtggag acgtttagat gggtaaaata
1801 attggtatcg acctgggtac taccaactct tgtgtagcga ttatggatgg caccactcct
1861 cgcgtgctgg agaacgccga aggcgatcgc accacgcctt ctatcattgc ctatacccag
1921 gatggtgaaa ctctagttgg tcagccggct aaacgtcagg cagtgacgaa cccgcaaaac
1981 actctgtttg cgattaaacg cctgattggt cgccgcttcc aggacgaaga agtacagcgt
2041 gatgtttcca tcatgccgtt caaaattatt gctgctgata acggcgacgc atgggtcgaa
2101 gttaaaggcc agaaaatggc accgccgcag atttctgctg aagtgctgaa aaaaatgaag
2161 aaaaccgctg aagattacct gggtgaaccg gtaactgaag ctgttatcac cgtaccggca
2221 tactttaacg atgctcagcg tcaggcaacc aaagacgcag gccgtatcgc tggtctggaa
2281 gtaaaacgta tcatcaacga accgaccgca gctgcgctgg cttacggtct ggacaaaggc
2341 actggcaacc gtactatcgc ggtttatgac ctgggtggtg gtactttcga tatttctatt
2401 atcgaaatcg acgaagttga cggcgaaaaa accttcgaag ttctggcaac caacggtgat
2461 acccacctgg ggggtgaaga cttcgacagc cgtctgatca actatctggt tgaagaattc
2521 aagaaagatc agggcattga cctgcgcaac gatccgctgg caatgcagcg cctgaaagaa
2581 gcggcagaaa aagcgaaaat cgaactgtct tccgctcagc agaccgacgt taacctgcca
2641 tacatcactg cagacgcgac cggtccgaaa cacatgaaca tcaaagtgac tcgtgcgaaa
2701 ctggaaagcc tggttgaaga tctggtaaac cgttccattg agccgctgaa agttgcactg
2761 caggacgctg gcctgtccgt atctgatatc gacgacgtta tcctcgttgg tggtcagact
2821 cgtatgccaa tggttcagaa gaaagttgct gagttctttg gtaaagagcc gcgtaaagac
2881 gttaacccgg acgaagctgt agcaatcggt gctgctgttc agggtggtgt tctgactggt
2941 gacgtaaaag acgtactgct gctggacgtt accccgctgt ctctgggtat cgaaaccatg
3001 ggcggtgtga tgacgacgct gatcgcgaaa aacaccacta tcccgaccaa gcacagccag
3061 gtgttctcta ccgctgaaga caaccagtct gcggtaacca tccatgtgct gcagggtgaa
3121 cgtaaacgtg cggctgataa caaatctctg ggtcagttca acctagatgg tatcaacccg
3181 gcaccgcgcg gcatgccgca gatcgaagtt accttcgata tcgatgctga cggtatcctg
3241 cacgtttccg cgaaagataa aaacagcggt aaagagcaga agatcaccat caaggcttct
3301 tctggtctga acgaagatga aatccagaaa atggtacgcg acgcagaagc taacgccgaa
3361 gctgaccgta agtttgaaga gctggtacag actcgcaacc agggcgacca tctgctgcac
3421 agcacccgta agcaggttga agaagcaggc gacaaactgc cggctgacga caaaactgct
3481 atcgagtctg cgctgactgc actggaaact gctctgaaag gtgaagacaa agccgctatc
3541 gaagcgaaaa tgcaggaact ggcacaggtt tcccagaaac tgatggaaat cgcccagcag
3601 caacatgccc agcagcagac tgccggtgct gatgcttctg caaacaacgc gaaagatgac
3661 gatgttgtcg acgctgaatt tgaagaagtc aaagacaaaa aataatcgcc ctataaacgg
3721 gtaattatac tgacacgggc gaaggggaat ttcctccccg cccgtgcatt catctagggg
3781 caatttaaaa aagatggcta agcaagatta ttacgagatt ttaggcgttt ccaaaacagc
3841 ggaagagcgt gaaatcagaa aggcctacaa acgcctggcc atgaaatacc acccggaccg
3901 taaccagggt gacaaagagg ccgaggcgaa atttaaagag atcaaggaag cttatgaagt
3961 tctgaccgac tcgcaaaaac gtgcggcata cgatcagtat ggtcatgctg cgtttgagca
4021 aggtggcatg ggcggcggcg gttctggcgg cggcgcagac ttcagcgata tttttggtga
4081 cgttttcggc gatatttttg gcggcggacg tggtcgtcaa cgtgcggcgc gcggtgctga
4141 tttacgctat aacatggagc tcaccctcga agaagctgta cgtggcgtga ccaaagagat
4201 ccgcattccg actctggaag agtgtgacgt ttgccacggt agcggtgcaa aaccaggtac
4261 acagccgcag acttgtccga cctgtcatgg ttctggtcag gtgcagatgc gccagggatt
4321 cttcgctgta cagcagacct gtccacactg tcagggccgc ggtacgctga tcaaagatcc
4381 gtgcaacaaa tgtcatggtc atggtcgtgt tgagcgcagc aaaacgctgt ccgttaaaat
4441 cccggcaggg gtggacactg gagaccgcat ccgtcttgcg ggcgaaggtg aagcgggcga
4501 gcatggcgca ccggcaggcg atctgtacgt tcaggttcag gttaaacagc acccgatttt
4561 cgagcgtgaa ggcaacaacc tgtattgcga agtcccgatc aacttcgcta tggcggcgct
4621 gggtggcgaa atcgaagtac cgacccttga tggtcgcgtc aaactgaaag tgcctggcga
4681 aacccagacc ggtaagctat tccgtatgcg cggtaaaggc gtcaagtctg tccgcggtgg
4741 cgcgcagggt gatttgctgt gccgcgttgt cgtcgaaaca ccggtaggcc tgaacgaaag
4801 gcagaaacag ctgctgcaag agctgcaaga aagcttcggt ggcccaaccg gcgagcacaa
4861 cagcccgcgc tcaaagagct tctttgatgg tgtgaagaag ttttttgacg acctgacccg
4921 ctaacctcgc ggagaaattc atgagtagta aagaacagaa aacgcctgag gggcaagccc
4981 cggaagaaat tatcatggat cagcacgaag agattgaggc agttgagcca gaagcttctg
5041 ctgagcaggt ggatccgcgc gatgaaaaag ttgcgaatct cgaagctcag ctggctgaag
5101 cccagacccg tgaacgtgac ggcattttgc gtgtaaaagc cgaaatggaa aacctgcgtc
5161 gtcgtactga actggatatt gaaaaagccc acaaattcgc gctggagaaa ttcatcaacg
5221 aattgctgcc ggtgattgat agcctggatc gtgcgctgga agtggctgat aaagctaacc
5281 cggatatgtc tgcgatggtt gaaggcattg agctgacgct gaagtcgatg ctggatgttg
5341 tgcgtaagtt tggcgttgaa gtgatcgccg aaactaacgt cccactggac ccgaatgtgc
5401 atcaggccat cgcaatggtg gaatctgatg acgttgcgcc aggtaacgta ctgggcatta
5461 tgcagaaggg ttatacgctg aatggtcgta cgattcgtgc ggcgatggtt actgtagcga
5521 aagcaaaagc ttaatttctg ctttcgtaat aattcacggc cctgcatgca agctcagatc
5581 tgagcttggc tgttttggcg gatgagagaa gaggtcgact ctagaggatc tactagtcat
5641 agcgccgata tcagatctgt tttcagcctg atacagatta aatcagaacg cagaagcggt
5701 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
5761 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
5821 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
5881 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
5941 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
6001 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
6061 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
6121 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
6181 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
6241 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
6301 aacagcggta agatccttga gagttttcgc cccgaagaac gcaagcttat cgatgataag
6361 ctgtcaaaca tgagaattac aacttatatc gtatggggct gacttcaggt gctacatttg
6421 aagagataaa ttgcactgaa atctagaaat attttatctg attaataaga tgatcttctt
6481 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg
6541 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag
6601 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga
6661 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc
6721 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
6781 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
6841 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
6901 agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt
6961 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg
7021 aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca
7081 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta
7141 gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg
7201 gtgcagcctt ttttctcctg ccacatgaag ccggtacccg gggatccgat ccgtttccat
7261 ttaggtgggt acgttggagc cgcattattt tcgctttatg aatctaaagg gtggttaact
7321 cgacatcttg gttaccgtga agttaccatc acggaaaaag gttatgctgc ttttaagacc
7381 cactttcaca tttaagttgt ttttctaatc cgcatatgat caattcaagg ccgaataaga
7441 aggctggctc tgcaccttgg tgatcaaata attcgatagc ttgtcgtaat aatggcggca
7501 tactatcagt agtaggtgtt tccctttctt ctttagcgac ttgatgctct tgatcttcca
7561 atacgcaacc taaagtaaaa tgccccacag cgctgagtgc atataatgca ttctctagtg
7621 aaaaaccttg ttggcataaa aaggctaatt gattttcgag agtttcatac tgtttttctg
7681 taggccgtgt acctaaatgt acttttgctc catcgcgatg acttagtaaa gcacatctaa
7741 aacttttagc gttattacgt aaaaaatctt gccagctttc cccttctaaa gggcaaaagt
7801 gagtatggtg cctatctaac atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt
7861 ttacatgcca atacaatgta ggctgctcta cacctagctt ctgggcgagt ttacgggttg
7921 ttaaaccttc gattccgacc tcattaagca gctctaatgc gctgttaatc actttacttt
7981 tatctaatct agacatcatt aattcctaat ttttgttgac actctatcgt tgatagagtt
8041 attttaccac tccctatcag tgatagagaa aagtgaaaat ccatatgact agtagatcct
8101 ctagagtcga ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
8161 cgaggccctt tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt ccctatcagt
8221 gatagagtat aatagagtcg aattgttagc ggagaagaat ttcacacaga attcattaaa
8281 gaggagaaag tcgaggcgtc acccataaca gatacggact ttctcaaagg agagttatca
8341 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa
8401 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg
8461 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa
8521 gttggcgaca tcgttatttt caacgatggc tacggtgtga aatctgagaa gatcgacaat
8581 gaagaagtgt tgatcatgtc cgaaagcgac attctggcaa ttgttgaagc gtaatccgcg
8641 cacgacactg aacatacgaa tttaaggaat aaagataatg gcagctaaag acgtaaaatt
8701 cggtaacgac gctcgtgtga aaatgctgcg cggcgtaaac gtactggcag atgcagtgaa
8761 agttaccctc ggtccaaaag gccgtaacgt agttctggat aaatctttcg gtgcaccgac
8821 catcaccaaa gatggtgttt ccgttgctcg tgaaatcgaa ctggaagaca agttcgaaaa
8881 tatgggtgcg cagatggtga aagaagttgc ctctaaagca aacgacgctg caggcgacgg
8941 taccaccact gcaaccgtac tggctcaggc tatcatcact gaaggtctga aagctgttgc
9001 tgcgggcatg aacccgatgg acctgaaacg tggtatcgac aaagcggtta ccgctgcagt
9061 tgaagaactg aaagcgctgt ccgtaccatg ctctgactct aaagcgattg ctcaggttgg
9121 taccatctcc gctaactccg acgaaaccgt aggtaaactg atcgctgaag cgatggacaa
9181 agtcggtaaa gaaggcgtta tcaccgttga agacggtacc ggtctgcagg acgaactgga
9241 cgtggttgaa ggtatgcagt tcgaccgtgg ctacctgtct ccttacttca tcaacaagcc
9301 ggaaactggc gcagtagaac tggaaagccc gttcatcctg ctggctgaca agaaaatctc
9361 caacatccgc gaaatgctgc cggttctgga agctgttgcc aaagcaggca aaccgctgct
9421 gatcatcgct gaagatgtag aaggcgaagc gctggcaact ctggttgtta acaccatgcg
9481 tggcatcgtg aaagtcgctg cggttaaagc accgggcttc ggcgatcgtc gtaaagctat
9541 gctgcaggat atcgcaaccc tgactggcgg taccgtgatc tctgaagaga tcggtatgga
9601 gctggaaaaa gcaaccctgg aagacctggg tcaggctaaa cgtgttgtga tcaacaaaga
9661 caccaccact atcatcgatg gcgtgggtga agaagctgca atccagggcc gtgttgctca
9721 gatccgtcag cagattgaag aagcaacttc tgactacgac cgtgaaaaac tgcaggaacg
9781 cgtagcgaaa ctggcaggcg gcgttgcagt tatcaaagtg ggtgctgcta ccgaagttga
9841 aatgaaagag aaaaaagcac gcgttgaaga tgccctgcac gcgacccgtg ctgcggtaga
9901 agaaggcgtg gttgctggtg gtggtgttgc gctgatccgc gtagcgtcta aactggctga
9961 cctgcgtggt cagaacgaag accagaacgt gggtatcaaa gttgcactgc gtgcaatgga
10021 agctccgctg cgtcagatcg tattgaactg cggcgaagaa ccgtctgttg ttgctaacac
10081 cgttaaaggc ggcgacggca actacggtta caacgcagca accgaagaat acggcaacat
10141 gatcgacatg ggtatcctgg atccaaccaa agtaactcgt tctgctctgc agtacgcagc
10201 ttctgtggct ggcctgatga tcaccaccga atgcatggtt accgacctgc cgaaaaacga
10261 tgcagctgac ttaggcgctg ctggcggtat gggcggcatg ggtggcatgg gcggcatgat
10321 gtaattgccc tgcacctcgc agaaataaac aaacccccgg gcagaaatgt ctgggggttt
10381 ttcttttggt catctttcta gtataaagat tcagacacgg acgacgcgag tggcgcctcg
10441 actagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
10501 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acctagactt
10561 cactgacacc ctcatcagtg ccaacatagt aagccagtat acactccgct agcgctgatg
10621 tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg agggacagct
10681 gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa atcagtaagt
10741 tggcagcatc acccgacgca ctttgcgccg aataaatacc tgtgacggaa gatcacttcg
10801 cagaataaat aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc
10861 gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
10921 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg
10981 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
11041 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
11101 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
11161 ttcttgcccg cctgatgaat gctcatccgg aattc