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- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pET-21c(+)(pET21c)
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
pET-21c(+)(pET21c)
质粒名称: pET-21c(+)
其他名称:
质粒大小（bp）: 5441
抗性:Ampicillin (氨苄青霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: 非病毒
启动子: T7
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: high
蛋白标签: N-T7, C-His
5’测序引物:T7
5’测序引物序列:5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性:
质粒图谱:
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
| 151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
| 201 | tccgacccat | ttgctgtcca | ccagtcatgc | tagccatatg | tatatctcct | 250 |
| 251 | tcttaaagtt | aaacaaaatt | atttctagag | gggaattgtt | atccgctcac | 300 |
| 301 | aattccccta | tagtgagtcg | tattaatttc | gcgggatcga | gatctcgatc | 350 |
| 351 | ctctacgccg | gacgcatcgt | ggccggcatc | accggcgcca | caggtgcggt | 400 |
| 401 | tgctggcgcc | tatatcgccg | acatcaccga | tggggaagat | cgggctcgcc | 450 |
| 451 | acttcgggct | catgagcgct | tgtttcggcg | tgggtatggt | ggcaggcccc | 500 |
| 501 | gtggccgggg | gactgttggg | cgccatctcc | ttgcatgcac | cattccttgc | 550 |
| 551 | ggcggcggtg | ctcaacggcc | tcaacctact | actgggctgc | ttcctaatgc | 600 |
| 601 | aggagtcgca | taagggagag | cgtcgagatc | ccggacacca | tcgaatggcg | 650 |
| 651 | caaaaccttt | cgcggtatgg | catgatagcg | cccggaagag | agtcaattca | 700 |
| 701 | gggtggtgaa | tgtgaaacca | gtaacgttat | acgatgtcgc | agagtatgcc | 750 |
| 751 | ggtgtctctt | atcagaccgt | ttcccgcgtg | gtgaaccagg | ccagccacgt | 800 |
| 801 | ttctgcgaaa | acgcgggaaa | aagtggaagc | ggcgatggcg | gagctgaatt | 850 |
| 851 | acattcccaa | ccgcgtggca | caacaactgg | cgggcaaaca | gtcgttgctg | 900 |
| 901 | attggcgttg | ccacctccag | tctggccctg | cacgcgccgt | cgcaaattgt | 950 |
| 951 | cgcggcgatt | aaatctcgcg | ccgatcaact | gggtgccagc | gtggtggtgt | 1000 |
| 1001 | cgatggtaga | acgaagcggc | gtcgaagcct | gtaaagcggc | ggtgcacaat | 1050 |
| 1051 | cttctcgcgc | aacgcgtcag | tgggctgatc | attaactatc | cgctggatga | 1100 |
| 1101 | ccaggatgcc | attgctgtgg | aagctgcctg | cactaatgtt | ccggcgttat | 1150 |
| 1151 | ttcttgatgt | ctctgaccag | acacccatca | acagtattat | tttctcccat | 1200 |
| 1201 | gaagacggta | cgcgactggg | cgtggagcat | ctggtcgcat | tgggtcacca | 1250 |
| 1251 | gcaaatcgcg | ctgttagcgg | gcccattaag | ttctgtctcg | gcgcgtctgc | 1300 |
| 1301 | gtctggctgg | ctggcataaa | tatctcactc | gcaatcaaat | tcagccgata | 1350 |
| 1351 | gcggaacggg | aaggcgactg | gagtgccatg | tccggttttc | aacaaaccat | 1400 |
| 1401 | gcaaatgctg | aatgagggca | tcgttcccac | tgcgatgctg | gttgccaacg | 1450 |
| 1451 | atcagatggc | gctgggcgca | atgcgcgcca | ttaccgagtc | cgggctgcgc | 1500 |
| 1501 | gttggtgcgg | atatctcggt | agtgggatac | gacgataccg | aagacagctc | 1550 |
| 1551 | atgttatatc | ccgccgttaa | ccaccatcaa | acaggatttt | cgcctgctgg | 1600 |
| 1601 | ggcaaaccag | cgtggaccgc | ttgctgcaac | tctctcaggg | ccaggcggtg | 1650 |
| 1651 | aagggcaatc | agctgttgcc | cgtctcactg | gtgaaaagaa | aaaccaccct | 1700 |
| 1701 | ggcgcccaat | acgcaaaccg | cctctccccg | cgcgttggcc | gattcattaa | 1750 |
| 1751 | tgcagctggc | acgacaggtt | tcccgactgg | aaagcgggca | gtgagcgcaa | 1800 |
| 1801 | cgcaattaat | gtaagttagc | tcactcatta | ggcaccggga | tctcgaccga | 1850 |
| 1851 | tgcccttgag | agccttcaac | ccagtcagct | ccttccggtg | ggcgcggggc | 1900 |
| 1901 | atgactatcg | tcgccgcact | tatgactgtc | ttctttatca | tgcaactcgt | 1950 |
| 1951 | aggacaggtg | ccggcagcgc | tctgggtcat | tttcggcgag | gaccgctttc | 2000 |
| 2001 | gctggagcgc | gacgatgatc | ggcctgtcgc | ttgcggtatt | cggaatcttg | 2050 |
| 2051 | cacgccctcg | ctcaagcctt | cgtcactggt | cccgccacca | aacgtttcgg | 2100 |
| 2101 | cgagaagcag | gccattatcg | ccggcatggc | ggccccacgg | gtgcgcatga | 2150 |
| 2151 | tcgtgctcct | gtcgttgagg | acccggctag | gctggcgggg | ttgccttact | 2200 |
| 2201 | ggttagcaga | atgaatcacc | gatacgcgag | cgaacgtgaa | gcgactgctg | 2250 |
| 2251 | ctgcaaaacg | tctgcgacct | gagcaacaac | atgaatggtc | ttcggtttcc | 2300 |
| 2301 | gtgtttcgta | aagtctggaa | acgcggaagt | cagcgccctg | caccattatg | 2350 |
| 2351 | ttccggatct | gcatcgcagg | atgctgctgg | ctaccctgtg | gaacacctac | 2400 |
| 2401 | atctgtatta | acgaagcgct | ggcattgacc | ctgagtgatt | tttctctggt | 2450 |
| 2451 | cccgccgcat | ccataccgcc | agttgtttac | cctcacaacg | ttccagtaac | 2500 |
| 2501 | cgggcatgtt | catcatcagt | aacccgtatc | gtgagcatcc | tctctcgttt | 2550 |
| 2551 | catcggtatc | attaccccca | tgaacagaaa | tcccccttac | acggaggcat | 2600 |
| 2601 | cagtgaccaa | acaggaaaaa | accgccctta | acatggcccg | ctttatcaga | 2650 |
| 2651 | agccagacat | taacgcttct | ggagaaactc | aacgagctgg | acgcggatga | 2700 |
| 2701 | acaggcagac | atctgtgaat | cgcttcacga | ccacgctgat | gagctttacc | 2750 |
| 2751 | gcagctgcct | cgcgcgtttc | ggtgatgacg | gtgaaaacct | ctgacacatg | 2800 |
| 2801 | cagctcccgg | agacggtcac | agcttgtctg | taagcggatg | ccgggagcag | 2850 |
| 2851 | acaagcccgt | cagggcgcgt | cagcgggtgt | tggcgggtgt | cggggcgcag | 2900 |
| 2901 | ccatgaccca | gtcacgtagc | gatagcggag | tgtatactgg | cttaactatg | 2950 |
| 2951 | cggcatcaga | gcagattgta | ctgagagtgc | accatatatg | cggtgtgaaa | 3000 |
| 3001 | taccgcacag | atgcgtaagg | agaaaatacc | gcatcaggcg | ctcttccgct | 3050 |
| 3051 | tcctcgctca | ctgactcgct | gcgctcggtc | gttcggctgc | ggcgagcggt | 3100 |
| 3101 | atcagctcac | tcaaaggcgg | taatacggtt | atccacagaa | tcaggggata | 3150 |
| 3151 | acgcaggaaa | gaacatgtga | gcaaaaggcc | agcaaaaggc | caggaaccgt | 3200 |
| 3201 | aaaaaggccg | cgttgctggc | gtttttccat | aggctccgcc | cccctgacga | 3250 |
| 3251 | gcatcacaaa | aatcgacgct | caagtcagag | gtggcgaaac | ccgacaggac | 3300 |
| 3301 | tataaagata | ccaggcgttt | ccccctggaa | gctccctcgt | gcgctctcct | 3350 |
| 3351 | gttccgaccc | tgccgcttac | cggatacctg | tccgcctttc | tcccttcggg | 3400 |
| 3401 | aagcgtggcg | ctttctcata | gctcacgctg | taggtatctc | agttcggtgt | 3450 |
| 3451 | aggtcgttcg | ctccaagctg | ggctgtgtgc | acgaaccccc | cgttcagccc | 3500 |
| 3501 | gaccgctgcg | ccttatccgg | taactatcgt | cttgagtcca | acccggtaag | 3550 |
| 3551 | acacgactta | tcgccactgg | cagcagccac | tggtaacagg | attagcagag | 3600 |
| 3601 | cgaggtatgt | aggcggtgct | acagagttct | tgaagtggtg | gcctaactac | 3650 |
| 3651 | ggctacacta | gaaggacagt | atttggtatc | tgcgctctgc | tgaagccagt | 3700 |
| 3701 | taccttcgga | aaaagagttg | gtagctcttg | atccggcaaa | caaaccaccg | 3750 |
| 3751 | ctggtagcgg | tggttttttt | gtttgcaagc | agcagattac | gcgcagaaaa | 3800 |
| 3801 | aaaggatctc | aagaagatcc | tttgatcttt | tctacggggt | ctgacgctca | 3850 |
| 3851 | gtggaacgaa | aactcacgtt | aagggatttt | ggtcatgaga | ttatcaaaaa | 3900 |
| 3901 | ggatcttcac | ctagatcctt | ttaaattaaa | aatgaagttt | taaatcaatc | 3950 |
| 3951 | taaagtatat | atgagtaaac | ttggtctgac | agttaccaat | gcttaatcag | 4000 |
| 4001 | tgaggcacct | atctcagcga | tctgtctatt | tcgttcatcc | atagttgcct | 4050 |
| 4051 | gactccccgt | cgtgtagata | actacgatac | gggagggctt | accatctggc | 4100 |
| 4101 | cccagtgctg | caatgatacc | gcgagaccca | cgctcaccgg | ctccagattt | 4150 |
| 4151 | atcagcaata | aaccagccag | ccggaagggc | cgagcgcaga | agtggtcctg | 4200 |
| 4201 | caactttatc | cgcctccatc | cagtctatta | attgttgccg | ggaagctaga | 4250 |
| 4251 | gtaagtagtt | cgccagttaa | tagtttgcgc | aacgttgttg | ccattgctgc | 4300 |
| 4301 | aggcatcgtg | gtgtcacgct | cgtcgtttgg | tatggcttca | ttcagctccg | 4350 |
| 4351 | gttcccaacg | atcaaggcga | gttacatgat | cccccatgtt | gtgcaaaaaa | 4400 |
| 4401 | gcggttagct | ccttcggtcc | tccgatcgtt | gtcagaagta | agttggccgc | 4450 |
| 4451 | agtgttatca | ctcatggtta | tggcagcact | gcataattct | cttactgtca | 4500 |
| 4501 | tgccatccgt | aagatgcttt | tctgtgactg | gtgagtactc | aaccaagtca | 4550 |
| 4551 | ttctgagaat | agtgtatgcg | gcgaccgagt | tgctcttgcc | cggcgtcaat | 4600 |
| 4601 | acgggataat | accgcgccac | atagcagaac | tttaaaagtg | ctcatcattg | 4650 |
| 4651 | gaaaacgttc | ttcggggcga | aaactctcaa | ggatcttacc | gctgttgaga | 4700 |
| 4701 | tccagttcga | tgtaacccac | tcgtgcaccc | aactgatctt | cagcatcttt | 4750 |
| 4751 | tactttcacc | agcgtttctg | ggtgagcaaa | aacaggaagg | caaaatgccg | 4800 |
| 4801 | caaaaaaggg | aataagggcg | acacggaaat | gttgaatact | catactcttc | 4850 |
| 4851 | ctttttcaat | attattgaag | catttatcag | ggttattgtc | tcatgagcgg | 4900 |
| 4901 | atacatattt | gaatgtattt | agaaaaataa | acaaataggg | gttccgcgca | 4950 |
| 4951 | catttccccg | aaaagtgcca | cctgaaattg | taaacgttaa | tattttgtta | 5000 |
| 5001 | aaattcgcgt | taaatttttg | ttaaatcagc | tcatttttta | accaataggc | 5050 |
| 5051 | cgaaatcggc | aaaatccctt | ataaatcaaa | agaatagacc | gagatagggt | 5100 |
| 5101 | tgagtgttgt | tccagtttgg | aacaagagtc | cactattaaa | gaacgtggac | 5150 |
| 5151 | tccaacgtca | aagggcgaaa | aaccgtctat | cagggcgatg | gcccactacg | 5200 |
| 5201 | tgaaccatca | ccctaatcaa | gttttttggg | gtcgaggtgc | cgtaaagcac | 5250 |
| 5251 | taaatcggaa | ccctaaaggg | agcccccgat | ttagagcttg | acggggaaag | 5300 |
| 5301 | ccggcgaacg | tggcgagaaa | ggaagggaag | aaagcgaaag | gagcgggcgc | 5350 |
| 5351 | tagggcgctg | gcaagtgtag | cggtcacgct | gcgcgtaacc | accacacccg | 5400 |
| 5401 | ccgcgcttaa | tgcgccgcta | cagggcgcgt | cccattcgcc | a | 5441 |
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pET-21c(+)(pET21c)
¥1 - 1880
