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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pET-20b(+)
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
pET-20b(+)
质粒名称: pET-20b(+)
其他名称:
质粒大小（bp）: 3716
抗性: Ampicillin (氨苄青霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-pelB信号肽, C-His
5’测序引物及序列: T7: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性:
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| T7_transl_en_RBS | 314 | 298 | |
| T7_promoter | 369 | 351 | |
| ROP | 878 | 1069 | |
| pGEX_3_primer | 1085 | 1063 | |
| pBR322_origin | 2103 | 1484 | |
| Ampicillin | 3118 | 2258 | |
| AmpR_promoter | 3188 | 3160 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 3118 | 2258 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| EcoRV | 217 |
| NcoI | 220 |
| NdeI | 288 |
| XbaI | 326 |
| BglII | 384 |
| PvuII | 1029 |
| PstI | 2576 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
| 151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
| 201 | tccgaattaa | ttccgatatc | catggccatc | gccggctggg | cagcgaggag | 250 |
| 251 | cagcagacca | gcagcagcgg | tcggcagcag | gtatttcata | tgtatatctc | 300 |
| 301 | cttcttaaag | ttaaacaaaa | ttatttctag | agggaaaccg | ttgtggtctc | 350 |
| 351 | cctatagtga | gtcgtattaa | tttcgcggga | tcgagatctc | gggcagcgtt | 400 |
| 401 | gggtcctggc | cacgggtgcg | catgatcgtg | ctcctgtcgt | tgaggacccg | 450 |
| 451 | gctaggctgg | cggggttgcc | ttactggtta | gcagaatgaa | tcaccgatac | 500 |
| 501 | gcgagcgaac | gtgaagcgac | tgctgctgca | aaacgtctgc | gacctgagca | 550 |
| 551 | acaacatgaa | tggtcttcgg | tttccgtgtt | tcgtaaagtc | tggaaacgcg | 600 |
| 601 | gaagtcagcg | ccctgcacca | ttatgttccg | gatctgcatc | gcaggatgct | 650 |
| 651 | gctggctacc | ctgtggaaca | cctacatctg | tattaacgaa | gcgctggcat | 700 |
| 701 | tgaccctgag | tgatttttct | ctggtcccgc | cgcatccata | ccgccagttg | 750 |
| 751 | tttaccctca | caacgttcca | gtaaccgggc | atgttcatca | tcagtaaccc | 800 |
| 801 | gtatcgtgag | catcctctct | cgtttcatcg | gtatcattac | ccccatgaac | 850 |
| 851 | agaaatcccc | cttacacgga | ggcatcagtg | accaaacagg | aaaaaaccgc | 900 |
| 901 | ccttaacatg | gcccgcttta | tcagaagcca | gacattaacg | cttctggaga | 950 |
| 951 | aactcaacga | gctggacgcg | gatgaacagg | cagacatctg | tgaatcgctt | 1000 |
| 1001 | cacgaccacg | ctgatgagct | ttaccgcagc | tgcctcgcgc | gtttcggtga | 1050 |
| 1051 | tgacggtgaa | aacctctgac | acatgcagct | cccggagacg | gtcacagctt | 1100 |
| 1101 | gtctgtaagc | ggatgccggg | agcagacaag | cccgtcaggg | cgcgtcagcg | 1150 |
| 1151 | ggtgttggcg | ggtgtcgggg | cgcagccatg | acccagtcac | gtagcgatag | 1200 |
| 1201 | cggagtgtat | actggcttaa | ctatgcggca | tcagagcaga | ttgtactgag | 1250 |
| 1251 | agtgcaccat | atatgcggtg | tgaaataccg | cacagatgcg | taaggagaaa | 1300 |
| 1301 | ataccgcatc | aggcgctctt | ccgcttcctc | gctcactgac | tcgctgcgct | 1350 |
| 1351 | cggtcgttcg | gctgcggcga | gcggtatcag | ctcactcaaa | ggcggtaata | 1400 |
| 1401 | cggttatcca | cagaatcagg | ggataacgca | ggaaagaaca | tgtgagcaaa | 1450 |
| 1451 | aggccagcaa | aaggccagga | accgtaaaaa | ggccgcgttg | ctggcgtttt | 1500 |
| 1501 | tccataggct | ccgcccccct | gacgagcatc | acaaaaatcg | acgctcaagt | 1550 |
| 1551 | cagaggtggc | gaaacccgac | aggactataa | agataccagg | cgtttccccc | 1600 |
| 1601 | tggaagctcc | ctcgtgcgct | ctcctgttcc | gaccctgccg | cttaccggat | 1650 |
| 1651 | acctgtccgc | ctttctccct | tcgggaagcg | tggcgctttc | tcatagctca | 1700 |
| 1701 | cgctgtaggt | atctcagttc | ggtgtaggtc | gttcgctcca | agctgggctg | 1750 |
| 1751 | tgtgcacgaa | ccccccgttc | agcccgaccg | ctgcgcctta | tccggtaact | 1800 |
| 1801 | atcgtcttga | gtccaacccg | gtaagacacg | acttatcgcc | actggcagca | 1850 |
| 1851 | gccactggta | acaggattag | cagagcgagg | tatgtaggcg | gtgctacaga | 1900 |
| 1901 | gttcttgaag | tggtggccta | actacggcta | cactagaagg | acagtatttg | 1950 |
| 1951 | gtatctgcgc | tctgctgaag | ccagttacct | tcggaaaaag | agttggtagc | 2000 |
| 2001 | tcttgatccg | gcaaacaaac | caccgctggt | agcggtggtt | tttttgtttg | 2050 |
| 2051 | caagcagcag | attacgcgca | gaaaaaaagg | atctcaagaa | gatcctttga | 2100 |
| 2101 | tcttttctac | ggggtctgac | gctcagtgga | acgaaaactc | acgttaaggg | 2150 |
| 2151 | attttggtca | tgagattatc | aaaaaggatc | ttcacctaga | tccttttaaa | 2200 |
| 2201 | ttaaaaatga | agttttaaat | caatctaaag | tatatatgag | taaacttggt | 2250 |
| 2251 | ctgacagtta | ccaatgctta | atcagtgagg | cacctatctc | agcgatctgt | 2300 |
| 2301 | ctatttcgtt | catccatagt | tgcctgactc | cccgtcgtgt | agataactac | 2350 |
| 2351 | gatacgggag | ggcttaccat | ctggccccag | tgctgcaatg | ataccgcgag | 2400 |
| 2401 | acccacgctc | accggctcca | gatttatcag | caataaacca | gccagccgga | 2450 |
| 2451 | agggccgagc | gcagaagtgg | tcctgcaact | ttatccgcct | ccatccagtc | 2500 |
| 2501 | tattaattgt | tgccgggaag | ctagagtaag | tagttcgcca | gttaatagtt | 2550 |
| 2551 | tgcgcaacgt | tgttgccatt | gctgcaggca | tcgtggtgtc | acgctcgtcg | 2600 |
| 2601 | tttggtatgg | cttcattcag | ctccggttcc | caacgatcaa | ggcgagttac | 2650 |
| 2651 | atgatccccc | atgttgtgca | aaaaagcggt | tagctccttc | ggtcctccga | 2700 |
| 2701 | tcgttgtcag | aagtaagttg | gccgcagtgt | tatcactcat | ggttatggca | 2750 |
| 2751 | gcactgcata | attctcttac | tgtcatgcca | tccgtaagat | gcttttctgt | 2800 |
| 2801 | gactggtgag | tactcaacca | agtcattctg | agaatagtgt | atgcggcgac | 2850 |
| 2851 | cgagttgctc | ttgcccggcg | tcaatacggg | ataataccgc | gccacatagc | 2900 |
| 2901 | agaactttaa | aagtgctcat | cattggaaaa | cgttcttcgg | ggcgaaaact | 2950 |
| 2951 | ctcaaggatc | ttaccgctgt | tgagatccag | ttcgatgtaa | cccactcgtg | 3000 |
| 3001 | cacccaactg | atcttcagca | tcttttactt | tcaccagcgt | ttctgggtga | 3050 |
| 3051 | gcaaaaacag | gaaggcaaaa | tgccgcaaaa | aagggaataa | gggcgacacg | 3100 |
| 3101 | gaaatgttga | atactcatac | tcttcctttt | tcaatattat | tgaagcattt | 3150 |
| 3151 | atcagggtta | ttgtctcatg | agcggataca | tatttgaatg | tatttagaaa | 3200 |
| 3201 | aataaacaaa | taggggttcc | gcgcacattt | ccccgaaaag | tgccacctga | 3250 |
| 3251 | aattgtaaac | gttaatattt | tgttaaaatt | cgcgttaaat | ttttgttaaa | 3300 |
| 3301 | tcagctcatt | ttttaaccaa | taggccgaaa | tcggcaaaat | cccttataaa | 3350 |
| 3351 | tcaaaagaat | agaccgagat | agggttgagt | gttgttccag | tttggaacaa | 3400 |
| 3401 | gagtccacta | ttaaagaacg | tggactccaa | cgtcaaaggg | cgaaaaaccg | 3450 |
| 3451 | tctatcaggg | cgatggccca | ctacgtgaac | catcacccta | atcaagtttt | 3500 |
| 3501 | ttggggtcga | ggtgccgtaa | agcactaaat | cggaacccta | aagggagccc | 3550 |
| 3551 | ccgatttaga | gcttgacggg | gaaagccggc | gaacgtggcg | agaaaggaag | 3600 |
| 3601 | ggaagaaagc | gaaaggagcg | ggcgctaggg | cgctggcaag | tgtagcggtc | 3650 |
| 3651 | acgctgcgcg | taaccaccac | acccgccgcg | cttaatgcgc | cgctacaggg | 3700 |
| 3701 | cgcgtcccat | tcgcca | 3716 |
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文献和实验相关实验
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pET-20b(+)
¥1 - 1880
