Anti-CaVα2δ2 (extracellular)

Anti-Ca_Vα2δ2 (extracellular)

Voltage-dependent calcium channel subunit alpha-2/delta-2
Cat #: ACC-1102
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IH, WB
May also work in: IC, IFC, IP, LCI
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
CLICK HERE TO RECEIVE A 25 µl FREE TRIAL SAMPLE!

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Ca_Vα2δ2. Anti-Ca_Vα2δ2 (extracellular) antibody (#ACC-102) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Ca_Vα2δ2 from rat, mouse and human samples.

For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.

• Applications
• Specifications
• Scientific Background
• Citations
• Related Products

Western blot

Western blot analysis of rat lung (lanes 1 and 6), rat brain (lanes 2 and 7), Mouse brain (lanes 3 and 8), rat heart (lanes 4 and 9) and mouse heart (lanes 5 and 10) lysates:
1-5. Anti-Ca_vα₂δ₂ (extracellular) antibody (#ACC-102), (1:200).
6-10. Anti-Ca_vα₂δ₂ (extracellular) antibody, preincubated  with the control  peptide antigen.
Immunohistochemistry

Expression of Cavα2δ2 in rat cerebellum
Immunohistochemical staining rat cerebellum using Anti-Cavα2δ2 (extracellular) antibody (#ACC-102). A. Cavα2δ2 (green) appears in the soma of purkinje cells (arrow) and in the molecular layer (M). B. Pre-incubation of the antibody with the control peptide antigen blocks staining of Purkinje cells and molecular layer. DAPI is used as the counterstain (blue).
Immunogen
(C)DLEAWAEKFKVLASNR, corresponding to amino acid residues 850-865 of  rat  Ca_vα₂δ₂ (Accession Q8CFG6). Extracellular, N-terminus.

HomologyMouse, human - identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution1 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Voltage-gated Ca²⁺ channels (Ca_V), enable the passage of Ca²⁺ ions in a voltage dependent manner. These heteromeric entities are formed in part by the pore-forming α1 subunit which determines the biophysical and pharmacological properties of the channel¹.

Ca_V1 and Ca_V2 channels are high-voltage activated (HVA) Ca_V channels. The a1 subunit of these channels normally interacts and associates with α2δ subunit, a membrane anchored protein and Ca_Vβ, a cytosolic protein¹.

Four α2δ subunits have been cloned to date: α2δ1- 4. This subunit originates from a single gene. The corresponding protein is modified by post-translational cleavage yielding a α2 subunit which is disulfide bonded to the δ subunit². All α2δ subunits are GPI- (glycosylphosphatidylinositol) anchored proteins³. The role of this subunit is important for the membrane trafficking of the α1 subunit, and also has a role in influencing the biophysical properties of the channel¹.

α2δ can be expressed as various splice variants and expressed in a tissue specific manner. α2δ2 can be detected in the brain, heart, lung, spleen and liver⁴.

Gabapentin and pregabalin are two commonly used anti-epileptic drugs. They act on Ca_V channels via the α2δ1 and α2δ2 subunits by disturbing their membrane trafficking, thereby decreasing Ca²⁺ currents.

References

1. Bauer, C.S. et al. (2010) Curr. Opin. Neurobiol. 20, 563.
2. Jay, S.D. et al. (1991) J. Biol. Chem. 266, 3287.
3. Davies, A. et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107, 1654.
4. Klugbauer, N. et al. (2003) J. Bioenerg. Biomembr. 35, 639.