Anti-CaVα2δ3 (extracellular)

Anti-Ca_Vα2δ3 (extracellular)

Voltage-dependent calcium channel subunit alpha-2/delta-3
Cat #: ACC-1103
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IC, IH, LCI, WB
May also work in: IFC, IP
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Ca_Vα2δ3. Anti-Ca_Vα2δ3 (extracellular) antibody (#ACC-103) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize Ca_Vα2δ3 from rat, mouse and human samples.

For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.

• Applications
• Specifications
• Scientific Background
• Citations
• Related Products

Western blot

Western blot analysis of  rat brain (lanes 1 and 3) and K562 (lanes 2 and 4) lysates:
1, 2. Anti-Ca_Vα2δ3 (extracellular) antibody (#ACC-103), (1:200).
3, 4. Anti-Ca_Vα2δ3 (extracellular) antibody preincubated with the control peptide antigen.
Live cell imaging / Immunocytochemistry
Rat PC12 cells (1:25). See immunocytochemistry.
Immunohistochemistry

Expression of Cavα2δ3 in rat hippocampus and cortex
Immunohistochemical staining of rat hippocampal CA3 region (A) and rat neocortex (B) using Anti-Cavα2δ3 (extracellular) antibody (#ACC-103). In both areas Cavα2δ3 staining (red) appears in pyramidal neurons (arrow). DAPI is used as the counterstain (blue).
Immunocytochemistry

Expression of Ca_Vα2δ3 in rat PC12 cells
Immunocytochemical staining of intact living PC12 cells. Extracellular staining of cells using Anti-Ca_vα2δ3 (extracellular) antibody (#ACC-103), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody.
Immunogen
Peptide CSWWHSDMTAKAQK, corresponding to amino acid residues 942-955 of rat Ca_Vα2δ3 (Accession Q8CFG5). Extracellular.

HomologyMouse, human - identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Voltage-gated Ca²⁺ (Ca_V) channels are ubiquitously expressed and function as Ca²⁺ conducting pores in the plasma membrane¹. On the basis of their voltage activation properties, Ca_V channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels². HVA channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca²⁺ flow across the membrane, and the auxiliary subunits α2δ, γ, and β³. The Ca²⁺ channel α2δ subunit is a heavily glycosylated protein that is encoded by a single gene and post-translationally cleaved to yield α2 and δ subunits linked by a disulfide bond with a single transmembrane segment⁴.  The α2δ subunit regulates many functional aspects of Ca²⁺ channels, such as gating, regulating voltage dependent kinetics, and increasing functional channel density on the plasma membrane⁵.

There are four proteins that comprise Ca_Vα2δ: Ca_Vα2δ1, Ca_Vα2δ2, Ca_Vα2δ3 and Ca_Vα2δ4⁶. The Ca_Vα2δ3 subunit is predominantly expressed in neuronal tissue. The Ca_Vα2δ3 subunit regulates all classes of HVA calcium channels. The Caα2δ3 subunits in the nerve terminal function in synaptic morphogenesis and cytoskeletal organization, and that this role is independent of their function in α1 subunit localization and physiology.

Ca_Vα2δ3 is likely to be the primary presynaptic α2δ isoform mediating morphological development of the neuromuscular junction (NMJ), since null alleles have such a large effect on NMJ development and abolish all action-potential evoked transmission⁷. Recent study shows that methylation-dependent transcriptional silencing of Ca_Vα2δ3 may contribute to the metastatic phenotype of breast cancer⁸.

References

1. Catterall, W.A. (2000) Annu. Rev. Cell. Dev. Biol. 16, 521.
2. Qin, N. et al. (2002) Mol. Pharmacol. 62, 485.
3. De Jongh, K.S. et al. (1990)   J. Biol. Chem. 265, 14738.
4. Sipos, I. et al. (2000) Pflug. Arch. 439, 691.
5. Dolphin, A.C. (2009) Curr. Opin. Neurobiol. 19, 237.
6. Cooper C.L. et al. (1987) J. Biol. Chem. 262, 509.
7. Kurshan P.T. (2009) Nat. Neurosci. 12, 1415.
8. Palmieri, C. et al. (2012) Br. J. Cancer 108, 375.