- $247 - 475
- Alomone
- 以色列
- ACC-105
- 2025年07月16日
- WB
- Rabbit
- 见官方网站
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
见官方网站
- 亚型：
见官方网站
- 形态：
液体或冻干粉
- 保存条件：
-20°C
- 克隆性：
多克隆
- 标记物：
见官方网站
- 适应物种：
见官方网站
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
ACC-105
- 级别：
高
- 库存：
大量
- 供应商：
上海信裕生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 浓度：
见官方网站
- 靶点：
见官方网站
- 抗体英文名：
Anti-CaVβ2
- 抗体名：
Anti-CaVβ2
- 规格：
25 µl, 50 µl, 0.2 ml
Anti-CaVβ2
Voltage-dependent L-type calcium channel subunit beta-2, CAB2, Cavβ2, CACB2Cat #: ACC-105
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: WB
May also work in: IC, IFC, IH, IP
Reactivity: M, R
May also work in: H
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blotSpecies reactivity key:
H- Human, M- Mouse, R- RatCLICK HERE TO RECEIVE A 25 µl FREE TRIAL SAMPLE!
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Cavβ2. Anti-CaVβ2 antibody (#ACC-105) can be used in western blot analysis, and has been designed to recognize Cavβ2 from rat, mouse and human samples.
For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.
- Applications
- Specifications
- Scientific Background
- Citations
- Related Products
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) membranes:
1,2. Anti-CaVβ2 antibody (#ACC-105), (1:800).
3,4. Anti-CaVβ2 antibody preincubated with the control peptide antigen.
Immunogen
Peptide (C)HEHVDRYVPHREHNHRE, corresponding to amino acid residues 571-587 of rat Cavβ2 (Accession Q8VGC3). Intracellular, C-terminus.
HomologyMouse - identical; human - 11/17 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.85 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background
Voltage-dependent Ca2+ channels are a family of membrane proteins that allow cells to couple electrical activity to intracellular Ca2+ signaling1. Voltage-gated Ca2+ channels are classified as T, L, N, P, Q and R, and are distinguished by their sensitivity to pharmacological blocks, single-channel conductance kinetics, and voltage-dependence.
On the basis of their voltage activation properties, voltage-gated Ca2+ subtypes can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels2. The activity of the channel pore is modulated by 4 tightly-coupled subunits: an intracellular β subunit; a transmembrane γ subunit; and a disulphide-linked complex of α2 and δ subunits3. There are four distinct β subunits: β1, β2, β3 and β44. There are 4 splice variants of the β2 subunit: β2a, β2b, β2c and β2d. β2a and β2b are expressed in heart, aorta and brain, and are 606- and 632-residue proteins, respectively. β2c and β2d (655 and 604 residues, respectively) are expressed in brain, and recent studies show beta-2c to be also expressed in heart. All splice variants differ in their N-terminal regions5.
References
- Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
- Qin, N. et al. (2002) Mol. Pharmacol. 62, 485.
- De Jongh, K.S. et al. (1990) J. Biol. Chem. 265, 14738.
- Pichler, M. et al. (1997) J. Biol. Chem. 272, 13877.
- Yamada, Y. et al. (2001) J. Biol. Chem. 276, 47163.
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