Anti-CaVβ2

Anti-Ca_Vβ2

Voltage-dependent L-type calcium channel subunit beta-2, CAB2, Ca_vβ2, CACB2
Cat #: ACC-105
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: WB
May also work in: IC, IFC, IH, IP
Reactivity: M, R
May also work in: H

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
CLICK HERE TO RECEIVE A 25 µl FREE TRIAL SAMPLE!

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Ca_vβ2. Anti-Ca_Vβ2 antibody (#ACC-105) can be used in western blot analysis, and has been designed to recognize Ca_vβ2 from rat, mouse and human samples.

For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.

• Applications
• Specifications
• Scientific Background
• Citations
• Related Products

Western blot

Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) membranes:
1,2. Anti-Ca_Vβ2 antibody (#ACC-105), (1:800).
3,4. Anti-Ca_Vβ2 antibody preincubated with the control peptide antigen.
Immunogen
Peptide (C)HEHVDRYVPHREHNHRE, corresponding to amino acid residues 571-587 of rat Ca_vβ2 (Accession Q8VGC3). Intracellular, C-terminus.

HomologyMouse - identical; human - 11/17 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.85 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Voltage-dependent Ca²⁺ channels are a family of membrane proteins that allow cells to couple electrical activity to intracellular Ca²⁺ signaling¹. Voltage-gated Ca²⁺ channels are classified as T, L, N, P, Q and R, and are distinguished by their sensitivity to pharmacological blocks, single-channel conductance kinetics, and voltage-dependence.

On the basis of their voltage activation properties, voltage-gated Ca²⁺ subtypes can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels². The activity of the channel pore is modulated by 4 tightly-coupled subunits: an intracellular β subunit; a transmembrane γ subunit; and a disulphide-linked complex of α2 and δ subunits³. There are four distinct β subunits: β1, β2, β3 and β4⁴. There are 4 splice variants of the β2 subunit: β2a, β2b, β2c and β2d. β2a and β2b are expressed in heart, aorta and brain, and are 606- and 632-residue proteins, respectively. β2c and β2d (655 and 604 residues, respectively) are expressed in brain, and recent studies show beta-2c to be also expressed in heart. All splice variants differ in their N-terminal regions⁵.

References

1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
2. Qin, N. et al. (2002) Mol. Pharmacol. 62, 485.
3. De Jongh, K.S. et al. (1990) J. Biol. Chem. 265, 14738.
4. Pichler, M. et al. (1997) J. Biol. Chem. 272, 13877.
5. Yamada, Y. et al. (2001) J. Biol. Chem. 276, 47163.