Anti-CaVβ1

Anti-Ca_Vβ1

Voltage-dependent L-type calcium channel subunit beta-1, CAB1, Cacnlb1
Cat #: ACC-106
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IH, WB
May also work in: IC, IP
Reactivity: M, R
May also work in: H

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Ca_Vβ1. Anti-Ca_Vβ1 antibody (#ACC-106) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Ca_Vβ1 from rat, mouse and human samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat cortex (lane 3 and 6):
1-3. Anti-Ca_Vβ1 antibody (#ACC-106), (1:800).
4-6. Anti-Ca_Vβ1 antibody preincubated with the control peptide antigen.
Immunohistochemistry

Expression of Ca_Vβ1 in rat hippocampus
Immunohistochemical staining of rat hippocampus using Anti-Ca_Vβ1 antibody (#ACC-106). A. Cavβ1 staining (red) appears in dendrites of the stratum radiatum (arrow).  B. Nuclear staining using DAPI as the counterstain (blue). C. Merge image of A and B.
Immunogen
Peptide (C)DRATGEHASVHEYPGE, corresponding to amino acid residues 456-471 of rat Ca_Vβ1 (Accession P54283). Intracellular, adjacent to the C-terminus.

HomologyMouse – identical; human - 14/16.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.85 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Voltage-gated Ca²⁺ (Ca_V) channels are ubiquitously expressed and function as Ca²⁺ conducting pores in the plasma membrane¹. Based on their electrophysiological and pharmacological properties, Ca_V channels have traditionally been classified into L, T, N, P, Q, and R types². L-type Ca²⁺ channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca²⁺ flow across the membrane, and the subunits α2δ, γ, and β³.

 Ca_Vβ subunits play critical roles in membrane trafficking of the channel complex and regulation of voltage-dependent gating. The Ca_Vβ subunit binds to the endoplasmic reticulum (ER) retention signal in the I-II loop of the α1-subunit, which allows channels to traffic to the surface membrane⁴. Furthermore, Ca_Vβ subunits not only allow for membrane trafficking of the channel complex, they also can play a role in determining the subcellular localization of channels on the surface membrane⁵.  There are four distinct Ca_Vβ subunits Ca_Vβ1, Ca_Vβ2, Ca_Vβ3 and Ca_Vβ4⁶.

Three splice variants exist for the β1 subunit: β1a, β1b and β1c. β1a is known to be expressed in skeletal muscle and brain, but not in smooth muscle or heart. β1a appears to be important for the functional expression of the α1 subunit in skeletal muscle⁷. β1b was identified by cloning in rat brain, heart and hippocampus, and differs from β1a by having a deletion of ~50 amino acids at residue 209, and having a 120-residue C-terminal elongation. β1c was cloned from human heart and hippocampus and has the same deletion as β1b, but lacks the C-terminal extension.

References

1. Catterall, W.A. et al. (2000) Annu. Rev. Cell. Dev. Biol. 16, 521.
2. Qin, N. et al. (2002) Mol. Pharmacol. 62, 485.
3. De Jongh, K.S. et al. (1990) J. Biol. Chem. 265, 14738.
4. Bichet, D. et al.  (2000) Neuron 25, 177.
5. Foell, J.D. et al. (2004) Physiol. Genomics 17, 183.
6. Dolphin, A.C. (2003) J. Bioenerg. Biomembr. 35, 599.
7. Beurg, M. et al. (1999) Biophys J. 77, 2953.