Anti-CaV3.1-ATTO-594

Anti-Ca_V3.1-ATTO-594

α_1G
Cat #: ACC-021-AR
Sizes: 50 µl
Source: Rabbit
Type: Polyclonal
Applications: IH
May also work in: IC
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Ca_V3.1 channel. Anti-Ca_V3.1 antibody (#ACC-021) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize Ca_V3.1 from human, rat and mouse samples.

Alomone Labs is pleased to offer a new version of this antibody that is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Ca_V3.1-ATTO-594 antibody (#ACC-021-AR) can be used in immunohistochemical staining. The conjugated antibody is ideal for immuno-colocalization studies.

• Applications
• Specifications
• Scientific Background
• Related Products

Immunohistochemistry

Expression of Ca_V3.1 in rat and mouse cerebellum
Immunohistochemical staining of mouse (A) and rat (B) cerebellum using Anti-Ca_V3.1-ATTO-594 antibody (#ACC-021-AR). A, B. Ca_V3.1 staining (red) appears in Purkinje neurons (arrow) and in the molecular layer (Mol). Nuclei were stained using DAPI as the counterstain (Blue).
Immunogen
Peptide MDEEEDGAGAEESGQPRSFTQL(C), corresponding to amino acid residues 1-22 of rat Ca_V3.1 (Accession O54898). Intracellular, N-terminus.

HomologyMouse - identical; human - 20/22 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
LabelATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
Standard quality control of each lotWestern blot analysis (unlabeled antibody, #ACC-021), and immunohistochemistry (labeled antibody).
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution50 µl double distilled water (DDW).
Antibody concentration after reconstitution1 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Voltage-dependent Ca²⁺ channels provide a pathway for rapid influx of Ca²⁺ into cells, which plays a crucial role in both electrical and metabolic signaling.¹ 

T-type currents are transduced via channel proteins encoded by three genes that compose a subfamily within the Cav channel family.^2-3 

The activity of T-type channels contributes to several known physiological and pathophysiological phenomena including burst firing in neurons, pace making activity in the heart and secretion from endocrine tissues.² There are three cloned T-type channel isoforms.  

Ca_V3.1 (α1G) and Ca_V3.2 (α1H) are widely distributed whereas the expression of Ca_V3.3 (α1I) is restricted to the central nervous system.²

Ca_V3.1 and Ca_V3.2 are also expressed in the kidney, but little is known about their physiological role there.

References

1. Serrano, J.R. et al. (1999) J. Gen. Physiol. 114, 185.
2. Perez-Reyes, E. (2003) Physiol. Rev. 83, 117.
3. Wain, H.M. et al. (2002) Nucleic Acids Res. 30, 169.
4. Andreasen, D. et al. (2000) Am. J. Physiol. 279, F997.