Anti-Aquaporin 1-ATTO-594

Anti-Aquaporin 1-ATTO-594

AQP1, CHIP28
Cat #: AQP-001-AR
Sizes: 50 µl
Source: Rabbit
Type: Polyclonal
Applications: IH
May also work in: IC
Reactivity: H, M, R

Anti-Aquaporin 1-ATTO-594Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Aquaporin 1 channel. Anti-Aquaporin 1 antibody (#AQP-001) can be used in western blot and immunohistochemical applications. It has been designed to recognize Aquaporin 1 from rat, mouse and human samples.

Alomone Labs is pleased to offer a new version of this antibody that is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Aquaporin 1-ATTO-594 antibody (#AQP-001-AR) can be used in immunohistochemical staining. The conjugated antibody is ideal for immuno-colocalization studies.

• Applications
• Specifications
• Scientific Background
• Related Products

Immunohistochemistry

Expression of Aquaporin 1 in rat kidney
Immunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 1-ATTO-594 antibody (#AQP-001-AR), (1:100). Aquaporin 1 staining (red) is present in convoluted tubules in the renal cortex. Glomeruli (G) are negative. Cell nuclei were visualized with Hoechst  33342 (blue).
Immunogen
Peptide (C)KVWTSGQVEEYDLDADDIN corresponding to amino acid residues 242-260 of human Aquaporin 1 (accession number P29972). Intracellular, C-terminus.

HomologyRat, mouse, cattle, sheep - identical; dog - 18/19 amino acid residues identical; frog (Rana esculenta), toad (Bufo marinus) - 14/19 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
LabelATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
Standard quality control of each lotWestern blot analysis (unlabeled antibody, #AQP-001), and immunohistochemistry (labeled antibody).
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution50 µl double distilled water (DDW).
Antibody concentration after reconstitution1 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Aquaporin 1 (AQP-1) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).

The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-1 together with AQP-2, AQP-4 and AQP-5 belongs to the first group.¹
Little is known about the function of the two newest members, AQP-11 and AQP-12.

The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.¹

AQP-1 is widely expressed in several organs with prominent expression found in kidney, lung, red blood cells intestine and brain. Studies with mice lacking AQP-1 show that the channel has a critical role in urine concentration.² In addition, AQP-1 expression in tumor cells was shown to contribute to enhanced tumor spread and metastatic potential.³

References

1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
2. Ma, T. et al. (1998) J Biol Chem 273, 4296.
3. Hu. J. and Verkman, A.S. (2006) FASEB J. 20, 1892.