MON R-5 is specific for human uPAR. No cross-reactivity is observed with murine uPAR, human uPA, human tPA or human PAI-1 when tested by ELISA and immunoblotting. MON R-5 reacts with domain 1 when tested by immunoprecipitation of proteolytic fragments of recombinant suPAR.
Immunogen
Native human uPAR
Application
ELISA, IHC
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass
IgG1
Gene ID
5329
Clone number
R-5
Epitope
MON R-5 reacts with an epitope on domain 1 and differs from that of MON R-3
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Urokinase plasminogen activator receptor (uPAR), also known as CD87, is a glycolipid anchored cell surface protein consisting of three homologous domains. It binds uPA with high affinity and enhances and localizes uPA catalyzed plasminogen activation, and thereby plays a key role in cancer invasion and in several tissue remodelling processes. In addition uPAR also binds to vitronectin and may thereby have biological functions independent of its role in uPA mediated proteolysis. Elevated levels of uPAR are associated with poor prognosis in many types of cancer.