Anti-Urokinase plasminogen act

Anti-Urokinase plasminogen activator (human, uPA)

Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。
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Cat. No.

MON U-6

Product name

Anti-Urokinase plasminogen activator (human, uPA)

Description

Mouse monoclonal antibody

Specificity

MON U-6 is specific for human uPA. No reaction with human tissue plasminogen activator (tPA) is seen when tested by ELISA, immunoblotting and enzyme inhibition. No reaction with any other human plasma proteins is seen when tested by immunoblotting. MON U-6 binds single and two-chain uPA, uPA/PAI-1 complex and uPA/uPAR complex. A reaction is seen with the amino terminal fragment (ATF) but not with LMW uPA. 

Immunogen

Native human uPA.

Application

ELISA, WB, IHC-F

Species reactivity (positive)

Human

Species reactivity (negative)

Not determined

Subclass

IgG1

Gene ID

5328

Clone number

U-6

Epitope

MON U-6 binds to the A-chain of uPA

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Urokinase plasminogen activator (uPA) is a serine protease. It converts the abundant proenzyme plasminogen to active plasmin and plays a key role in cancer invasion and a variety of tissue remodelling processes such as wound healing, mammary gland involution and placental development. Elevated levels of uPA are associated with poor prognosis in many types of cancer (1).

Other information

This product is not for further manufacture.

Application 1

ELISA
MON U-6 can be used in ELISA (1, 2, 3).
 

Application 2

WB
MON U-6 reacts with reduced and non-reduced uPA in Western blotting. It inhibits the enzymatic activity of uPA in the fibrin plate assay. 

Application 3

IHC
MON U-6 can be used on frozen sections in immunohistochemistry applications (4).