MON U-6 is specific for human uPA. No reaction with human tissue plasminogen activator (tPA) is seen when tested by ELISA, immunoblotting and enzyme inhibition. No reaction with any other human plasma proteins is seen when tested by immunoblotting. MON U-6 binds single and two-chain uPA, uPA/PAI-1 complex and uPA/uPAR complex. A reaction is seen with the amino terminal fragment (ATF) but not with LMW uPA.
Immunogen
Native human uPA.
Application
ELISA, WB, IHC-F
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass
IgG1
Gene ID
5328
Clone number
U-6
Epitope
MON U-6 binds to the A-chain of uPA
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Urokinase plasminogen activator (uPA) is a serine protease. It converts the abundant proenzyme plasminogen to active plasmin and plays a key role in cancer invasion and a variety of tissue remodelling processes such as wound healing, mammary gland involution and placental development. Elevated levels of uPA are associated with poor prognosis in many types of cancer (1).
Other information
This product is not for further manufacture.
Application 1
ELISA MON U-6 can be used in ELISA (1, 2, 3).
Application 2
WB MON U-6 reacts with reduced and non-reduced uPA in Western blotting. It inhibits the enzymatic activity of uPA in the fibrin plate assay.
Application 3
IHC MON U-6 can be used on frozen sections in immunohistochemistry applications (4).