- $410
- 丹麦BioPorto Diagnostics A/S
- 丹麦
- HYB 314-01
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4-8ºC without exposure to light. No precautions necessary during handling.
- 保质期：
12个月
- 库存：
大量
- 供应商：
上海信裕生物科技有限公司
- 抗体英文名：
Anti-Gliadin peptide
- 规格：
200ug/1mg
Anti-Gliadin peptide
- Cat. No.
- HYB 314-01
- Product name
- Anti-Gliadin peptide
- Description
-
Mouse monoclonal antibody
- Specificity
-
HYB 314-01 reacts strongly with the deamidated peptide but cross-reacts about 5% with the non-deamidated peptide KLQPFPQPQLPYPQPQ.
- Immunogen
-
Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ)
- Application
- ELISA, WB
- Species reactivity (positive)
- Wheat, Rye, Barley
- Species reactivity (negative)
- Oat
- Subclass
-
IgG2a/k
- Clone number
- 14D5
- Epitope
-
The epitope is located in a central region of the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide (1).
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
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文献和实验Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
二个以上的α -氨基酸以肽键连接起来而形成的化合物。根据构成氨基酸的数目 2、 3、 4……叫做二肽（ dipeptide）、三肽、四肽……。大体上由 10以下的肽键形成的肽总称为寡肽 （ Oligopeptide），由多数肽键构成的肽总称为多肽。每个肽按照构成它的氨基酸的名称，从具有游离α -氨基的末端氨基酸依次命名。例如 称为甘氨酰丙氨酸的二肽。蛋白质是由一条或几条长链状多肽构成的。象谷胱甘肽和肌肽那样的低
Purification Of Anti Peptide Antibody
Purification Of Anti Peptide Antibody Claire Walczak January 1996 Protocol: All steps up to the dialysis at rt. Pour column in TBS (=0.15M NaCl, 20mM TrisCl pH 7.4). We use a 5 ml column for 25 mls
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