Anti-Complement component C1s

Anti-Complement component C1s (human, pro- and activated enzyme)

Cat. No.

ABS 002-49

Product name

Anti-Complement component C1s (human, pro- and activated enzyme)

Description

Mouse monoclonal antibody

Specificity

ABS 002-49 is specific for C1s proenzyme and activated free C1s and C1s in complex with C1 inhibitor.

Immunogen

Human plasma C1s Proenzyme

Application

ELISA, IF

Species reactivity (positive)

Human

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

716

Clone number

49

Epitope

Epitope differs from that of ABS 002-09

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

C1s proenzyme is a 79.8 kDa glycoprotein produced primarily by hepatocytes but also by other cells. The proenzyme is produced as a single-chain polypeptide of 688 aa. Upon activation the peptide is cleaved between R437 and I438 giving the activated enzyme consisting of two disulfide-linked chains, A chain (52.2 kDa) and B chain (27.7 kDa). The Ca-dependent catalytic subunit of the C1 complex (C1q, C1r, C1s) comprises of two C1s and two C1r molecules and this serine protease tetramer is responsible for the initial steps in the classical complement pathway, cleavage of C4 and C2.