HYB 029-03 is specific for human C5. No reaction is seen with plasma from patients deficient in C5.
Immunogen
C5 isolated from human plasma
Application
ELISA, WB
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass
IgG1/k
Gene ID
727
Clone number
11F6
Epitope
Epitope specificity differs from that of HYB 029-02 but slightly overlap as determined by inhibition ELISA.
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9). The molecular mass of C5 is 190 kDa.
Application 1
ELISA
HYB 029-03 reacts strongly with C5. A strong reaction is seen with normal human plasma, and no reaction is seen with plasma from patients deficient in C5, when tested in sandwich ELISA in combination with a polyclonal antibody against C5 (eg. DAKO A 055).
Application 2
WB
In Western blotting after SDS-PAGE, HYB 029-03 reacts with C5 in normal human plasma (PEG precipitated C5) in nonreduced form only and no reaction is seen with plasma from C5 deficient patients