*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification
About the Assay
Format
96-well microplate with reagents sufficient to test 40 samples in duplicate
Species Reactivity
Rabbit, Pig, Sheep, Goat, Cow, Horse, Cynomolgus Monkey, Human Cell Culture, Guinea Pig No recognition of Rat, Mouse, or Dog
Assay Steps
Add 25 µL Standards, Controls, and Samples Add 125 µL anti-Osteocalcin Antibody Incubate 2 hours at room temperature Wash 3 times with 1X Wash Buffer Add 150 µL reconstituted Enzyme Conjugate Incubate 60 minutes at room temperature Wash 3 times with 1X Wash Buffer Add 150 µL Working Substrate Solution Incubate 35-40 minutes at room temperature Add 50 µL Stop Solution Read Optical Density at 405 nm Data Reduction: 4 Parameter