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- 详细信息
- 技术资料
- 供应商:
上海再康生物科技有限公司
- 库存:
大量
- 规格:
1 kit
Features & Benefits
| Feature | Benefit |
|---|
Product Specifications
| Format | ELISA |
| Test/kit | 96 wells/plate |
| Sample type | Serum |
Performance Characteristics
Method: Competitive
Analyte: Intact osteocalcin
Specimen: Serum
Specimen Volume: 25 µL
LOD*: 0.45 ng/L
LLOQ*: 2 ng/mL
HLOQ*: 32 ng/L
Precision CVs within-run: 5%-10%
Precision CVs between-run: 5%-10%
Incubation Time: 3.6 Hours
Cross-reactivity: Intact OC 100% Reduced OC 0% N-, C- peptides 0%
*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification
About the Assay
Format
96-well microplate with reagents sufficient to test 40 samples in duplicate
Species Reactivity
Rabbit, Pig, Sheep, Goat, Cow, Horse, Cynomolgus Monkey, Human Cell Culture, Guinea Pig
No recognition of Rat, Mouse, or Dog
Assay Steps
Add 25 µL Standards, Controls, and Samples
Add 125 µL anti-Osteocalcin Antibody
Incubate 2 hours at room temperature
Wash 3 times with 1X Wash Buffer
Add 150 µL reconstituted Enzyme Conjugate
Incubate 60 minutes at room temperature
Wash 3 times with 1X Wash Buffer
Add 150 µL Working Substrate Solution
Incubate 35-40 minutes at room temperature
Add 50 µL Stop Solution
Read Optical Density at 405 nm
Data Reduction: 4 Parameter
Ordering Information
| Catalog Number | Description |
|---|---|
| 8002 | ELISA Kit |
Product Documentation
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