HYB 008-02 is specific for the Bb fragment of human factor B. Antibody binding abolishes the formation of C3bBb complexes.
Immunogen
Complement factor B purified from human plasma
Application
ELISA, WB
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass
IgG1/k
Gene ID
629
Clone number
9B8
Epitope
Differs from that of HYB 008-06. Also differs from HYB 008-04 and HAV 005-02, but with slight overlap as determined by some mutual inhibition of antibody binding.
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Factor B is a component of the alternative pathway of complement activation. The protein consists of a single polypeptide chain of molecular mass 93 kDa, and has a concentration in plasma of approximately 200 µg/mL. Factor B is cleaved by factor D into the Ba component (30 kDa) and the Bb (63 kDa). Together with C3b, Bb forms the alternative pathway C3 convertase C3bBb.
Application 1
ELISA
HYB 008-02 reacts strongly with human factor B. A strong reaction is seen when used as a biotinylated detection antibody in sandwich ELISA with a polyclonal capture antibody against factor B (e.g. Dako A0343).
Application 2
WB
After SDS-PAGE, HYB 008-02 reacts with the Bb fragment of factor B in its non-reduced form only. HYB 008-02 can be used for the affinity purification of human factor B, when coupled to a suitable matrix.