Anti-Complement component C5b-

Anti-Complement component C5b-9 (human)

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Cat. No.

DIA 011-019

Product name

Anti-Complement component C5b-9 (human)

Description

Mouse monoclonal antibody

Specificity

DIA 011-01 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive) (1). 

Immunogen

Purified C5b-9 

Application

ELISA, WB (not applicable), IHC-F, IHC-P, IF

Species reactivity (positive)

Human, pig, horse, baboon

Species reactivity (negative)

Not determined

Subclass

IgG2a/k

Gene ID

727

Clone number

aE11

Epitope

DIA 011-01 binds to a neoepitope exposed on C9 

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and DIA 011-01 is directed against a neoepitope exposed on C9 when incorporated into the TCC.

Other information

This product is not for further manufacture.

Application 1

ELISA
DIA 011-01 is well suited for quantifying TCC in ELISA. (2,3)

Application 2

WB
DIA 011-01 is not recommended for Western blotting as the epitope is destroyed during the process (4).

Application 3

IHC
TCC in various tissues by immunohistochemistry (frozen and paraffin sections) (1, 2, 4).

IHC staining of frozen tonsil section. Vascular endothelia and dendritic cells of germinal center have strong and intensive label. Dilution: 1:10.

 

Application 4

IF
DIA 011-01 can be used in immunofluorescence staining (5).