HYB 345-02 reacts with CCK-8 and the other CCK analogs with identical C-terminal ends as well as Gastrin II. No reactivity was observed with human heparin cofactor II.
Immunogen
CCK-8 fragment 26-33 GGDRDY(SO3H)MGWMDF
Application
ELISA, IF
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass
IgG2b/k
Gene ID
885
Clone number
27F3..0,5D10
Epitope
27F3..0,5D10
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Cholecystokinin plays a key role in facilitating digestion within the small intestine. It is secreted from mucosal epithelial cells in the first segment of the small intestine (duodenum). Cholecystokinin stimulates delivery into the small intestine of digestive enzymes from the pancreas and bile from the gallbladder.Cholecystokinin is also produced by neurons in the enteric nervous system, and is widely and abundantly distributed in the brain.Cholecystokinin is a linear peptide that is synthesized as a preprohormone, which is proteolytically cleaved to generate a family of peptides having the same carboxy ends. In all of these CCK peptides, the tyrosine 7 residue from the end is sulfated, which is necessary for activity. One of these peptides is the octa-peptide CCK-8 retaining full biologic activity.
Application 1
ELISA HYB 345-02 was used in ELISA.
Application 2
Immunofluorescence (IF) HYB 345-02 was used in IF (1).