HYB 228-08 i specific for human AT III, native and denatured.
Immunogen
Antithrombin III isolated from human plasma
Application
ELISA,IHC
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass
IgG1/k
Gene ID
462
Clone number
2 E10
Epitope
Not determined
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components.
Application 1
ELISA
HYB 228-08 reacts strongly with AT III both native and denatured forms. A strong reaction is seen in ELISA with both native and denatured AT III directly coated onto the microtiter well and also when used as detection antibody in sandwich ELISA in combination with a polyclonal capture antibody (eg. DAKO A 296).
Application 2
IHC
HYB 228-08 can be used in IHC. Please consult www.proteinatlas.org
Paraffin-embedded human liver tissue immunostained with HYB 228-08 (www.proteinatlas.org)
Paraffin-embedded human cancerous liver tissue immunostained with HYB 228-08 (www.proteinatlas.org)