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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
见官方网站
- 亚型:
见官方网站
- 形态:
冻干粉或液体
- 保存条件:
-20ºC
- 克隆性:
多克隆
- 标记物:
见官方网站
- 适应物种:
见官方网站
- 保质期:
1年
- 抗原来源:
见官方网站
- 目录编号:
HYB 076-082
- 级别:
见官方网站
- 库存:
30
- 供应商:
上海泽叶生物科技有限公司
- 宿主:
Mycobacterium tuberculosis
- 应用范围:
ELISA, WB
- 浓度:
1 mg/ml
- 靶点:
见官方网站
- 抗体英文名:
Anti-Mycobacterium tuberculosis ESAT-6
- 抗体名:
Anti-Mycobacterium tuberculosis ESAT-6
- 规格:
200ug
Anti-Mycobacterium tuberculosis ESAT-6
Bioporto是一家体外诊断公司,专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站:www.bioporto.com
- Cat. No.
- HYB 076-082
- Product name
- Anti-Mycobacterium tuberculosis ESAT-6
- Description
- Mouse monoclonal antibody
- Specificity
- HYB 076-08 reacts with ESAT-6 in immunoblotting after SDS-PAGE of short-term culture filtrates of Mycobacterium tuberculosis and virulent strains of M. bovis. HYB 076-08 cross-reacts with MPB64 (26 kDa) from certain BCG substrains, with the A, B, and C components of the antigen 85 complex and with MPT51. H
- Immunogen
- Purified protein derivative (PPD) of tuberculin from M. tuberculosis conjugated with thimerosal N-hydroxysuccinimide ester
- Application
- ELISA, WB
- Species reactivity (positive)
- Mycobacterium tuberculosis
- Species reactivity (negative)
- Not determined
- Subclass
- IgG1/k
- Clone number
- 11 G4
- Epitope
- Binds to residues 3-15 (EQQWNFAGIEAAA) of ESAT-6 (1)
- Content
- Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
- ESAT-6 (6-kDa early secretory antigenic target) from M. tuberculosis H37Rv (ATCC 27294) (1) is a secreted tubercular protein that forms a dominant target for cell-mediated immunity in the early phase of infection. The deduced amino acid sequence indicates a molecular mass of 10 kDa. It is present in M. tuberculosis, virulent strains of M. bovis, and in M. flavescens, M. kansasii, M. marinum,and M. szulgai, but not in avirulent M. bovisBCG substrains (3).
- Application 1
-
ELISA
HYB 076-08 can be used in ELISA (2 ). - Application 2
-
WB
HYB 076-08 reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis (2, 3, 4).
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文献和实验The Mycobacterium tuberculosis Phagosome
Tuberculosis is currently the most devastating human bacterial disease, causing millions of deaths annually and infecting an overwhelming percentage of the global population. Its success as a scourge lies in the ability of Mycobacterium
Preparation of Culture Filtrate Proteins from Mycobacterium tuberculosis
Culture filtrates obtained by in vitro cultivation of Mycobacterium tuberculosis have been studied for more than 20 years to identify and characterize proteins of immunological relevance. Culture filtrate preparations have been shown
Analysis of the Capsule of Mycobacterium tuberculosis
Mycobacterium tuberculosis and the other pathogenic mycobacteria examined so far by electron microscopy are seen to be surrounded within host cells by an electron-transparent zone (ETZ). For Mycobacterium lepraemurium , this space
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