- $940
- BioPorto
- 丹麦
- BW 015
- 2025年07月16日
- ELISA
- 见官方网站
- 见官方网站
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
见官方网站
- 亚型：
见官方网站
- 形态：
冻干粉或液体
- 保存条件：
-20ºC
- 克隆性：
多克隆
- 标记物：
见官方网站
- 适应物种：
见官方网站
- 保质期：
1年
- 抗原来源：
见官方网站
- 目录编号：
BW 015
- 级别：
见官方网站
- 库存：
30
- 供应商：
上海泽叶生物科技有限公司
- 宿主：
见官方网站
- 应用范围：
ELISA
- 浓度：
1 mg/ml
- 靶点：
见官方网站
- 抗体英文名：
"Total GLP-1" Matched Antibody Pair
- 抗体名：
"Total GLP-1" Matched Antibody Pair
"Total GLP-1" Matched Antibody Pair
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- BW 015
- Product name
- "Total GLP-1" Matched Antibody Pair
- Description
-
Mouse monoclonal antibodies
- Specificity
-
This uses HYB 147-06 as capture antibody in combination with biotinylated detection antibody HYB 147-12B to measure the sum of GLP-1(7-36)amide, GLP-1(1-36)amide and GLP-1(9-36)amide in biological samples. Relative to measuring the two active forms of GLP-1, the responses are reduced by not measuring the contribution of GLP-1(7- 37) to the overall response, but this is compensated for by measuring an approximately equal contribution from GLP-1(1-36)amide. However, the GLP-1(9-36)amide degradation product is also measured, augmenting the response above that of the active forms alone (1).
HYB 147-06-02 495 € HYB 147-12B-005 380 € Total 875 € Matched Pair Price 785 €
- Immunogen
-
Synthetic GLP-1(7-36)amide
- Application
- ELISA
- Content
-
200 µL capture antibody and 50 µl detection antibody. 1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Target
-
Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.
- Application 1
-
ELISA
HYB 147-06 (as capture antibody) forms a sandwich ELISA pair with HYB 147-12B (as biotinylated detection antibody) for measuring total GLP-1.
This uses HYB 147-06 as capture antibody in combination with biotinylated detection antibody HYB 147-12B to measure the sum of GLP-1(7-36)amide, GLP-1(1-36)amide and GLP-1(9-36)amide in biological samples. Relative to measuring the two active forms of GLP-1, the responses are reduced by not measuring the contribution of GLP-1(7- 37) to the overall response, but this is compensated for by measuring an approximately equal contribution from GLP-1(1-36)amide. However, the GLP-1(9-36)amide degradation product is also measured, augmenting the response above that of the active forms alone.
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文献和实验. However, if no antibody is available for antigen of which the coding sequence is known, Northern blotting is the procedure of choice to quantify differences in gene expression at the transcriptional level. This is the second-best solution, since the mRNA level
Identification of a Mutant Kinase/ATP Analog Pair
(if [y-32 P]ATP analog is used) or immunoblotted with a phosphospecific antibody (if available) to determine the extent of substrate phosphorylation. The kinase/ATP analog pair that provides the best phosphorylation of the substrate is then used
A. Plating: To sterilize glass coverslips, dip in ethanol and flame. We use 22x22x1 mm3 coverslips and put them in 6-well plates. Seed 100,000 cells per well overnight and fix the next day. B. Fixation: Remove the media
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