pHT01

pHT01

载体基本信息

出品公司:	Ybscience
载体名称:	pHT01
质粒类型:	枯草芽孢杆菌表达载体
高拷贝/低拷贝:	--
启动子:	--
克隆方法:	多克隆位点，限制性内切酶
载体大小:	--
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	--
筛选标记:	--
备注:	--
产品目录号:	--
稳定性:	--
组成型:	--
病毒/非病毒:	--

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       pHT01	7956 bp     DNA  circular	SYN
DEFINITION  pHT01
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="pHT01"
                     /mol_type="other DNA"
     CDS             complement(113..763)
                     /label="ORF frame 3"
     misc_feature    complement(1110..1258)
                     /label="lacZ_a"
     misc_feature    1224..1246
                     /label="M13_pUC_fwd_primer"
     promoter        1239..1255
                     /label="M13_forward20_primer"
     misc_feature    complement(1298..2377)
                     /label="lacI"
     CDS             complement(1298..2380)
                     /label="ORF frame 3"
     terminator      2833..2856
                     /label="trpA_terminator"
     CDS             3883..4917
                     /label="ORF frame 1"
     CDS             complement(5293..5799)
                     /label="ORF frame 1"
     promoter        6068..6096
                     /label="AmpR_promoter"
     gene            6138..6998
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             6138..6998
                     /label="ORF frame 3"
     rep_origin      7153..7772
                     /label="pBR322_origin"
ORIGIN
    1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
   61 tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa
  121 gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat
  181 aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt
  241 tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat
  301 ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag
  361 gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt
  421 ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt
  481 tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc
  541 cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga
  601 aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa
  661 tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct
  721 cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa
  781 tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc
  841 ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat
  901 ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaaaga ccacattaaa
  961 aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc cttttctttc
 1021 ttatcttgat aataagggta actattgccg atcgtccatt ccgacagcat cgccagtcac
 1081 tatggcgtgc tgctagcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
 1141 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
 1201 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
 1261 cgagctcagg ccttaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
 1321 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
 1381 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
 1441 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
 1501 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
 1561 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
 1621 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
 1681 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
 1741 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
 1801 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
 1861 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
 1921 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
 1981 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
 2041 gattgtgcac cgccgtttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
 2101 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
 2161 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
 2221 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
 2281 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
 2341 cggcatactc tgcgacatcg tataacgtta ctggtttcat caaaatcgtc tccctccgtt
 2401 tgaatatttg attgatcgta accagatgaa gcactctttc cactatccct acagtgttat
 2461 ggcttgaaca atcacgaaac aataattggt acgtacgatc tttcagccga ctcaaacatc
 2521 aaatcttaca aatgtagtct ttgaaagtat tacatatgta agatttaaat gcaaccgttt
 2581 tttcggaagg aaatgatgac ctcgtttcca ccggaattag cttggtacca gctattgtaa
 2641 cataatcggt acgggggtga aaaagctaac ggaaaaggga gcggaaaaga atgatgtaag
 2701 cgtgaaaaat tttttatctt atcacttgaa attggaaggg agattcttta ttataagaat
 2761 tgtggaattg tgagcggata acaattccca attaaaggag gaaggatcct ctagagtcga
 2821 cgtccccggg gcagcccgcc taatgagcgg gcttttttca cgtcacgcgt ccatggagat
 2881 ctttgtctgc aactgaaaag tttatacctt acctggaaca aatggttgaa acatacgagg
 2941 ctaatatcgg cttattagga atagtccctg tactaataaa atcaggtgga tcagttgatc
 3001 agtatatttt ggacgaagct cggaaagaat ttggagatga cttgcttaat tccacaatta
 3061 aattaaggga aagaataaag cgatttgatg ttcaaggaat cacggaagaa gatactcatg
 3121 ataaagaagc tctaaaacta ttcaataacc ttacaatgga attgatcgaa agggtggaag
 3181 gttaatggta cgaaaattag gggatctacc tagaaagcca caaggcgata ggtcaagctt
 3241 aaagaaccct tacatggatc ttacagattc tgaaagtaaa gaaacaacag aggttaaaca
 3301 aacagaacca aaaagaaaaa aagcattgtt gaaaacaatg aaagttgatg tttcaatcca
 3361 taataagatt aaatcgctgc acgaaattct ggcagcatcc gaagggaatt catattactt
 3421 agaggatact attgagagag ctattgataa gatggttgag acattacctg agagccaaaa
 3481 aactttttat gaatatgaat taaaaaaaag aaccaacaaa ggctgagaca gactccaaac
 3541 gagtctgttt ttttaaaaaa aatattagga gcattgaata tatattagag aattaagaaa
 3601 gacatgggaa taaaaatatt ttaaatccag taaaaatatg ataagattat ttcagaatat
 3661 gaagaactct gtttgttttt gatgaaaaaa caaacaaaaa aaatccacct aacggaatct
 3721 caatttaact aacagcggcc aaactgagaa gttaaatttg agaaggggaa aaggcggatt
 3781 tatacttgta tttaactatc tccattttaa cattttatta aaccccatac aagtgaaaat
 3841 cctcttttac actgttcctt taggtgatcg cggagggaca ttatgagtga agtaaaccta
 3901 aaaggaaata cagatgaatt agtgtattat cgacagcaaa ccactggaaa taaaatcgcc
 3961 aggaagagaa tcaaaaaagg gaaagaagaa gtttattatg ttgctgaaac ggaagagaag
 4021 atatggacag aagagcaaat aaaaaacttt tctttagaca aatttggtac gcatatacct
 4081 tacatagaag gtcattatac aatcttaaat aattacttct ttgatttttg gggctatttt
 4141 ttaggtgctg aaggaattgc gctctatgct cacctaactc gttatgcata cggcagcaaa
 4201 gacttttgct ttcctagtct acaaacaatc gctaaaaaaa tggacaagac tcctgttaca
 4261 gttagaggct acttgaaact gcttgaaagg tacggtttta tttggaaggt aaacgtccgt
 4321 aataaaacca aggataacac agaggaatcc ccgattttta agattagacg taaggttcct
 4381 ttgctttcag aagaactttt aaatggaaac cctaatattg aaattccaga tgacgaggaa
 4441 gcacatgtaa agaaggcttt aaaaaaggaa aaagagggtc ttccaaaggt tttgaaaaaa
 4501 gagcacgatg aatttgttaa aaaaatgatg gatgagtcag aaacaattaa tattccagag
 4561 gccttacaat atgacacaat gtatgaagat atactcagta aaggagaaat tcgaaaagaa
 4621 atcaaaaaac aaatacctaa tcctacaaca tcttttgaga gtatatcaat gacaactgaa
 4681 gaggaaaaag tcgacagtac tttaaaaagc gaaatgcaaa atcgtgtctc taagccttct
 4741 tttgatacct ggtttaaaaa cactaagatc aaaattgaaa ataaaaattg tttattactt
 4801 gtaccgagtg aatttgcatt tgaatggatt aagaaaagat atttagaaac aattaaaaca
 4861 gtccttgaag aagctggata tgttttcgaa aaaatcgaac taagaaaagt gcaataaact
 4921 gctgaagtat ttcagcagtt ttttttattt agaaatagtg aaaaaaatat aatcagggag
 4981 gtatcaatat ttaatgagta ctgatttaaa tttatttaga ctggaattaa taattaacac
 5041 gtagactaat taaaatttaa tgagggataa agaggataca aaaatattaa tttcaatccc
 5101 tattaaattt taacaagggg gggattaaaa tttaattaga ggtttatcca caagaaaaga
 5161 ccctaataaa atttttacta gggttataac actgattaat ttcttaatgg gggagggatt
 5221 aaaatttaat gacaaagaaa acaatctttt aagaaaagct tttaaaagat aataataaaa
 5281 agagctttgc gattaagcaa aactctttac tttttcattg acattatcaa attcatcgat
 5341 ttcaaattgt tgttgtatca taaagttaat tctgttttgc acaacctttt caggaatata
 5401 aaacacatct gaggcttgtt ttataaactc agggtcgcta aagtcaatgt aacgtagcat
 5461 atgatatggt atagcttcca cccaagttag cctttctgct tcttctgaat gtttttcata
 5521 tacttccatg ggtatctcta aatgattttc ctcatgtagc aaggtatgag caaaaagttt
 5581 atggaattga tagttcctct ctttttcttc aactttttta tctaaaacaa acactttaac
 5641 atctgagtca atgtaagcat aagatgtttt tccagtcata atttcaatcc caaatctttt
 5701 agacagaaat tctggacgta aatcttttgg tgaaagaatt tttttatgta gcaatatatc
 5761 cgatacagca ccttctaaaa gcgttggtga atagggcatt ttacctatct cctctcattt
 5821 tgtggaataa aaatagtcat attcgtccat ctacctatcc tattatcgaa cagttgaact
 5881 ttttaatcaa ggatcagtcc tttttttcat tattcttaaa ctgtgctctt aactttaaca
 5941 actcgatttg tttttccaga tctcgagggt aactagcctc gccgatcccg caagaggccc
 6001 ggcagtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
 6061 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
 6121 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
 6181 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
 6241 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
 6301 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
 6361 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
 6421 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
 6481 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
 6541 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
 6601 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
 6661 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
 6721 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
 6781 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
 6841 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
 6901 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
 6961 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
 7021 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
 7081 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
 7141 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
 7201 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
 7261 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
 7321 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
 7381 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
 7441 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
 7501 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
 7561 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
 7621 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
 7681 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
 7741 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
 7801 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
 7861 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
 7921 tgagcgagga agcggaagag cgcccaatac gcatgc