pLKO-DEST-YFP

pLKO-DEST-YFP

载体基本信息

出品公司:	Ybscience
载体名称:	pLKO-DEST-YFP 或 pLKO.DEST.YFP
质粒类型:	慢病毒载体；RNAi载体；cDNA/shRNA gateway
高拷贝/低拷贝:	低拷贝
克隆方法:	gateway
启动子:	U6
载体大小:	8897 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素、氯霉素
筛选标记:	YFP
克隆菌株:	阳性筛选用DB3.1 ，阴性筛选用OmniMAX2-T1, 重组慢病毒载体的复制用Stbl3感受态细胞，以维持质粒的稳定性。
宿主细胞（系）:	哺乳动物细胞
备注:	其它筛选标记 pLKO-DEST-EGFP                         pLKO-DEST-puro                         pLKO-DEST-hygro
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	慢病毒

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       pLKO.DEST.YFP	8897 bp 	DNA	SYN
DEFINITION  pLKO.DEST.YFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="pLKO.DEST.YFP"
                     /mol_type="other DNA"
     misc_feature    complement(114..221)
                     /label="attR1"
     misc_feature    114..214
                     /label="attR2"
     misc_feature    complement(300..323)
                     /label="MSCV_rev_primer"
     misc_feature    704..723
                     /label="mPGK_F_primer"
     CDS             796..1689
                     /label="ORF frame 1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPAIGSLVDRITDLS
                     PQGDPGRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGRCHLRQADPEVHLH
                     HRQAARALAHPRDHLRLRPAVLRPLPRPHEAARLLQVRHARRLRPGAHHLLQGRRQLQ
                     DPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLYHGRQAEERHQGEL
                     QDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPELPVRPEQRPQREARSHGPAGV
                     RDRRRDHSRHGRAVQVKRPRL*"
     CDS             953..1672
                     /label="ORF frame 2"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     gene            956..1663
                     /label="EYFP"
                     /gene="EYFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(998..1019)
                     /label="EGFP_N_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    1544..1582
                     /label="T203Y (EYFP)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    1606..1627
                     /label="EGFP_C_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2022..2037
                     /label="cPPT"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2022..2043
                     /label="U3PPT"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2039..2091
                     /label="delta_U3"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2092..2272
                     /label="HIV-1_5_LTR"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2092..2272
                     /label="truncHIV-1_3_LTR"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     terminator      2347..2466
                     /label="SV40_PA_terminator"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2435..2454
                     /label="EBV_rev_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     rep_origin      2484..2582
                     /label="SV40_origin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2567..2586
                     /label="SV40pro_F_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     rep_origin      2798..3104
                     /label="f1_origin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     promoter        3297..3325
                     /label="AmpR_promoter"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     gene            3367..4227
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     CDS             3367..4227
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4347..4931
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        5242..5260
                     /label="M13_reverse_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        5277..5296
                     /label="T3_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5550..5730
                     /label="truncHIV-1_3_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5550..5730
                     /label="HIV-1_5_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5841..5885
                     /label="HIV-1_psi_pack"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             6273..6872
                     /label="ORF frame 3"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     misc_feature    6395..6628
                     /label="RRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     promoter        7165..7397
                     /label="hU6_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     misc_feature    7327..7346
                     /label="LKO1_5_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     misc_feature    7431..7555
                     /label="attR1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     misc_feature    complement(7455..7555)
                     /label="attR2"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     promoter        7580..7603
                     /label="lac_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     gene            7664..8323
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     CDS             7664..8323
                     /label="ORF frame 2"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    8665..73
                     /label="ccdB"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
ORIGIN
    1 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
   61 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
  121 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
  181 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
  241 atgggcggcc gctctagagg gcccaagctt acgaggcgct tttcccaagg cagtctggag
  301 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
  361 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
  421 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
  481 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
  541 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
  601 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
  661 gcggggcggg cgcccgaagg tcctccggag gcccggtgtt ccgcattctg cacgcttcaa
  721 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
  781 ccaagctagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
  841 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc catcggctcc
  901 ctcgttgacc gaatcaccga cctctctccc cagggggatc ccggtcgcca ccatggtgag
  961 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
 1021 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
 1081 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
 1141 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
 1201 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
 1261 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
 1321 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
 1381 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
 1441 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
 1501 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
 1561 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
 1621 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
 1681 cgactctagc atgtttaagg gttccggttc cactaggtac aattcgatat caagcttatc
 1741 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
 1801 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
 1861 cgtatggctt tcattttctc ctccttgacc ggagcttacc cgcccgcccc acgacccgca
 1921 gcgcccgacc gaaaggagcg cacgacccca tgcatcgtta agagctcggt acctttaaga
 1981 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
 2041 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
 2101 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
 2161 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
 2221 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
 2281 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
 2341 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
 2401 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
 2461 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
 2521 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
 2581 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
 2641 tagggacgta cccaattcgc ctggcgttac ccaacttaat cgccttgcag cacatccccc
 2701 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
 2761 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
 2821 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
 2881 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
 2941 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
 3001 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
 3061 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
 3121 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
 3181 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
 3241 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
 3301 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
 3361 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
 3421 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
 3481 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
 3541 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
 3601 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
 3661 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
 3721 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
 3781 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
 3841 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
 3901 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
 3961 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
 4021 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
 4081 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
 4141 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
 4201 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
 4261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
 4321 ctcatgagga agcggaagag cgctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
 4381 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaaggtaa
 4441 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
 4501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
 4561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
 4621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
 4681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
 4741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
 4801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
 4861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
 4921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
 4981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
 5041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
 5101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
 5161 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
 5221 ctcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
 5281 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
 5341 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
 5401 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
 5461 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
 5521 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
 5581 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
 5641 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
 5701 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
 5761 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
 5821 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
 5881 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
 5941 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
 6001 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
 6061 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
 6121 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
 6181 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
 6241 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
 6301 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
 6361 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
 6421 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
 6481 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
 6541 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
 6601 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
 6661 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
 6721 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
 6781 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
 6841 ataaatgggc aagtttgtgg aattgggttt aacataacaa attggctgtg gtatataaaa
 6901 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
 6961 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
 7021 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
 7081 agatccattc gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgagcg
 7141 gccgccccct tcaccgaggg cctatttccc atgattcctt catatttgca tatacgatac
 7201 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
 7261 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
 7321 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
 7381 atcttgtgga aaggacgaaa caccggccgg tgatatcctc gagcccatca acaagtttgt
 7441 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
 7501 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
 7561 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
 7621 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
 7681 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
 7741 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
 7801 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
 7861 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
 7921 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
 7981 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
 8041 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
 8101 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
 8161 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
 8221 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
 8281 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
 8341 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
 8401 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
 8461 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
 8521 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
 8581 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
 8641 ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc
 8701 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
 8761 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
 8821 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
 8881 gtctccgtta tcgggga