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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8度保存
- 保质期:
3年
- 库存:
大量
- 供应商:
盖宁生物
- 规格:
500T
CCK-8法与普通的MTT法相比,具有显著特点:
★灵敏度高,数据可靠,重现性好
★操作简便,省时省力
★水溶性,不需要换液,尤其适合于悬浮细胞
★无需放射性同位素和有机溶剂,对细胞毒性低
★为1瓶溶液,毋需预制,即开即用
★适合于高通量药物筛选
CCK-8用途:药物筛选、细胞增殖测定、细胞毒性测定、肿瘤药敏试验
CCK-8试剂盒在国内知名科研院所、重点大学、三甲医院被广泛应用,国际认可度高,使用CCK-8发表的中外文献达600多篇,其中截止08年底SCI影响因子IF>10分的论文有37篇,最高IF38.5分
[Heterozygosity with respect to Zfp148 causes complete loss of fetal germ cells during mouse embryogenesis,Nature Genetics 33, 172 - 176 (01 Feb 2003)]
CCK-8试剂与MTT等方法灵敏度比较

CCK-8试剂毒性:CCK-8和其他检测方法对比可以得出——CCK-8对细胞几乎无毒性,不伤害细胞,细胞可重复利用


CCK-8 P公司产
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文献和实验Overview Count the number of viable cells in a culture via dilution plating. The following assumes E. coli cells but it should apply to any type of cells. Materials LB agar plate LB media for dilution
Cell counting with an hemacytometer.
Inc. Garden grove CA 92841 Hemacytometer Fisher scientific No 02671 5 Dilute your cell sample in Trypan Blue dye exclusion medium. Dead cells will be blue, live cells will be birefringent. Suggested dilution 1:20 (5µl cell suspension in 95 µl Trypan Blue
Using a Counting Chamber For microbiology, cell culture, and many applications that require use of suspensions of cells it is necessary to determine cell concentration. One can often determine cell density of a suspension
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