pLVX-TetOne-Luc

pLVX-TetOne-Luc

载体基本信息

出品公司:	Ybscience
载体名称:	pLVX-TetOne-Luc
质粒类型:	慢病毒载体；四环素调控载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	TRE3GS,  hPGK
载体大小:	9935  bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	无
载体抗性:	氨苄青霉素
筛选标记:	无
克隆菌株:	Stbl3
宿主细胞（系）:	常规细胞系（293、CV-1、CHO等）
备注:	慢病毒载体pLVX-TetOne-Luc是pLVX-TetOne的阳性对照载体。
稳定性:	稳表达
组成型/诱导型:	诱导型
病毒/非病毒:	慢病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       pLVX-TetOne-Luc           9935 bp ds-DNA    circular SYN 24-SEP-2013
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     631844, 631845, 631846
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="5' LTR"
     misc_feature    681..806
                     /note="Packaging signal"
     misc_feature    1303..1536
                     /note="RRE" 
     misc_feature    2028..2144
                     /note="cPPT/CTS"
     polyA_signal    2187..2321
                     /note="SV40 polyA signal"
     CDS             complement(2522..4174)
                     /label="luciferase"
                     /note="firefly luciferase"
     promoter        complement(4184..4548)
                     /note="TRE3GS promoter"
     promoter        4568..5078
                     /note="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             5097..5843
                     /note="Tet-On 3G"
     misc_feature    5857..6445
                     /note="WPRE"
     LTR             6653..7286
                     /note="3' LTR"
     rep_origin      complement(7817..8402)
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8573..9433)
                     /note="AmpR"
     promoter        complement(9434..9538)
                     /note="AmpR promoter"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc catcgataga tctgcggccg
     2521 cttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg atttttcttg
     2581 cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact cctccgcgca
     2641 actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt tccgtcatcg
     2701 tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca tcgtcgggaa
     2761 gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgac tccaattcag
     2821 cgggggccac ctggtatcct ttgtatttaa ttaaagactt caagcggtca actatgaaga
     2881 agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca tccttgtcaa
     2941 tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata ggtcctctga
     3001 cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga tccacaacct
     3061 tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc ccctcgggtg
     3121 taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc cctggaagat
     3181 ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa
     3241 tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg aagaatgaaa
     3301 atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca
     3361 gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa tatccgagtg
     3421 tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca gtatccggaa
     3481 tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag gcagttctat
     3541 gcggaagggc cacaccctta ggtaacccag tagatccaga ggagttcatt atcagtgcaa
     3601 ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa ccgggaggta
     3661 gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt ttagaatcca
     3721 tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt ttttgcaacc
     3781 cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg agcaattcac
     3841 gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca
     3901 ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga tttgtattca
     3961 gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat tccgcgtacg
     4021 tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc agggcgtatc
     4081 tcttcatagc cttatgcagt tgctctccag cggttccatc ctcaagagga tagaatggcg
     4141 ccgggccttt ctttatgttt ttggcgtctt ccatggtgaa ttctttacga gggtaggaag
     4201 tggtacggaa agttggtata agacaaaagt gttgtggaat tgaagtttac tcaaaaaatc
     4261 agcactcttt tataggcgcc ctggtttaca taagcaaagc ttatacgttc tctatcactg
     4321 atagggagta aactggatat acgttctcta tcactgatag ggagtaaact gtagatacgt
     4381 tctctatcac tgatagggag taaactggtc atacgttctc tatcactgat agggagtaaa
     4441 ctccttatac gttctctatc actgataggg agtaaagtct gcatacgttc tctatcactg
     4501 atagggagta aactcttcat acgttctcta tcactgatag ggagtaaact cgaggtgata
     4561 attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     4621 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     4681 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     4741 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     4801 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     4861 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     4921 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     4981 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     5041 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatcatcga attaccatgt
     5101 ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta
     5161 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     5221 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     5281 ggcatcatac ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     5341 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     5401 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     5461 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     5521 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     5581 ccaccgattc tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag
     5641 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     5701 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     5761 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     5821 accttgacat gctccccggg taaacgcgtc tggaacaatc aacctctgga ttacaaaatt
     5881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6241 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     6301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6361 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6421 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     6481 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     6541 cacaagagga ggaggaggtg ggtttttcca gtcacacctc aggtaccttt aagaccaatg
     6601 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
     6661 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     6721 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     6781 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     6841 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     6901 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     6961 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     7021 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     7081 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     7141 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7201 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7261 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7321 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac
     7381 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7441 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7501 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8341 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9541 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
     9601 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9661 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9721 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     9781 actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     9841 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttt ttaaagaaat
     9901 tgtatttgtt aaatatgtac tacaaactta gtagt