pLVX-EF1α-IRES-mCherry

pLVX-EF1α-IRES-mCherry

载体基本信息

出品公司:	Ybscience
载体名称:	pLVX-EF1α-IRES-mCherry, pLVX-EF1a-IRES-mCherry, pLVX-EF1alpha-IRES-mCherry
质粒类型:	慢病毒载体；哺乳细胞表达
高拷贝/低拷贝:	高拷贝
启动子:	EF1α/ EF1a
克隆方法:	多克隆位点，限制性内切酶
载体大小:	8904 bp
5' 测序引物及序列:	EF1a Forward: TCAAGCCTCAGACAGTGGTTC
3' 测序引物及序列:	IRES-R: CCTCACATTGCCAAAAGACG
载体标签:	无标签
载体抗性:	氨苄青霉素
筛选标记:	mCherry红色荧光蛋白
克隆菌株:	Stbl3  E.coli
备注:	含有IRES元件；目的基因与mCherry基因共转录，独立翻译。
稳定性:	稳表达
组成型:	组成型
病毒/非病毒:	慢病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       pLVX-EF1a-IRES-mCherry   8904 bp    DNA   circular            12-FEB-2010
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 631987
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_binding    636..653
                     /label=PBS
     misc_feature    685..822
                     /label=Packaging\signal
     misc_binding    1303..1536
                     /label=RRE
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     misc_feature    3535..3572
                     /label=MCS
     misc_feature    3574..4148
                     /label=IRES
     misc_feature    4873..5464
                     /label=WPRE
     LTR             5667..6303
                     /label=3'\LTR
     CDS             4149..4859
                     /label=mCherry
     rep_origin      complement(6772..7445)
                     /label=pUC\origin
     CDS             complement(7590..8586)
                     /label=Amp(R)
     promoter        2185..3519
                     /label=EF1a\Promoter
BASE COUNT     2209 a      2201 c      2385 g      2109 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 
     3541 ctcgagacta gttctagagc ggccgcggat cccgcccctc tccctccccc ccccctaacg 
     3601 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 
     3661 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 
     3721 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 
     3781 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 
     3841 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 
     3901 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 
     3961 gagtcaaatg gctcacctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 
     4021 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga 
     4081 ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg 
     4141 atgataatat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 
     4201 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 
     4261 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc 
     4321 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg 
     4381 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt 
     4441 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc 
     4501 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg 
     4561 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg 
     4621 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact 
     4681 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct 
     4741 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac 
     4801 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtgaa 
     4861 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 
     4921 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 
     4981 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 
     5041 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 
     5101 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 
     5161 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 
     5221 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 
     5281 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 
     5341 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 
     5401 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 
     5461 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata 
     5521 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt 
     5581 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta 
     5641 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag 
     5701 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca 
     5761 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag 
     5821 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg 
     5881 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc 
     5941 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat 
     6001 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg 
     6061 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact 
     6121 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
     6181 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
     6241 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
     6301 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 
     6361 gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg 
     6421 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 
     6481 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 
     6541 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 
     6601 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 
     6661 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
     6721 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
     6781 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     6841 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     6901 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     6961 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     7021 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     7081 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     7141 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     7201 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
     7261 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     7321 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     7381 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     7441 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
     7501 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
     7561 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 
     7621 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
     7681 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 
     7741 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
     7801 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
     7861 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 
     7921 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
     7981 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
     8041 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
     8101 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
     8161 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 
     8221 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
     8281 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
     8341 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
     8401 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 
     8461 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 
     8521 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 
     8581 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag 
     8641 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     8701 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca 
     8761 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca 
     8821 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta 
     8881 aatatgtact acaaacttag tagt