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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pLVX-mCherry-C1
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pLVX-mCherry-C1
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pLVX-mCherry-C1 |
| 质粒类型: | 慢病毒表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8778 bp |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen) |
| 3' 测序引物及序列: | -- |
| 载体标签: | N-mCherry |
| 载体抗性: | 氨苄 |
| 筛选标记: | 嘌呤霉素(Puromycin) |
| 备注: | 该载体要用 Stbl3 感受态细胞进行质粒扩增，条件为30度。 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-mCherry-C1 8778 bp DNA circular 7-OCT-2009
COMMENT Created by Clontech Laboratories Inc.
COMMENT Cat. No. 632561
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
misc_feature 4746..5337
/label=WPRE
CDS complement(7464..8460)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
LTR 5540..6176
/label=3'\LTR
promoter 3604..4112
/label=PGK\Promoter
CDS 4133..4732
/label=Puro(R)
misc_feature 3589..3591
/label=Stop
misc_feature 3593..3595
/label=Stop
CDS 2807..3514
/label=mCherry
misc_feature 2028..2151
/label=cPPT/CTS\(DNA\flap)
promoter 2185..2787
/label=CMV\IE\Promoter
rep_origin complement(6646..7319)
/label=pUC\ori
misc_feature 3597..3599
/label=Stop
misc_feature 3528..3580
/label=MCS
BASE COUNT 2181 a 2271 c 2330 g 1996 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatgg tgagcaaggg
2821 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
2881 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
2941 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
3001 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
3061 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
3121 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
3181 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
3241 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
3301 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
3361 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
3421 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
3481 ccactccacc ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc
3541 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat
3601 cataattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
3661 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
3721 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
3781 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
3841 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
3901 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
3961 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
4021 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
4081 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
4141 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
4201 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
4261 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
4321 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
4381 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
4441 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
4501 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
4561 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
4621 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
4681 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct
4741 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
4801 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
4861 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
4921 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
4981 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
5041 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
5101 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
5161 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
5221 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
5281 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
5341 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
5401 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
5461 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
5521 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
5581 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
5641 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
5701 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
5761 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
5821 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
5881 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
5941 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
6001 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
6061 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
6121 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
6181 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
6241 agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca
6301 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
6361 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
6421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
6481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
7141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
7741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
7801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
7861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
7921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
7981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
8041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
8101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
8161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
8221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
8281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
8341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
8401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
8461 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
8521 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
8581 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
8641 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
8701 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
8761 tactacaaac ttagtagt
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pLVX-mCherry-C1
¥800 - 1200
