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-20℃
6个月
pCDH-EF1-Luc2-T2A-tdTomato
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
载体名称: | pCDH-EF1-Luc2-T2A-tdTomato |
质粒类型: | 哺乳动物细胞载体;慢病毒包装载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | EF1 alpha |
载体大小: | 9.5 kb |
5' 测序引物及序列: | EF1引物:CTCCACGCTTTGCCTGACCCTGCTT |
3' 测序引物及序列: | -- |
载体标签: | C-Luciferase |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) |
备注: | 包装质粒是pLP1、pLP2、pLP/VSVG。 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 慢病毒 |
LOCUS pCDH-EF1-Luc2-T2A-tdTomato 9565 bp ds-DNA circular SYN 30-AUG-2016DEFINITION pCDH-EF1-Luc2-T2A-tdTomato vectorCOMMENT This file is created by Vector NTI http://www.biofeng.com/COMMENT ORIGDB|GenBankCOMMENT VNTAUTHORNAME|biofeng.com|FEATURES Location/Qualifiers source 1..9565 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..26) /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 500..604 /gene="bla" /note="AmpR promoter" CDS 605..1465 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" rep_origin 1636..2224 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2512..2533 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 2548..2578 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 2586..2602 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2610..2626 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin 2664..2799 /note="SV40 ori" /note="SV40 origin of replication" polyA_signal 2818..2939 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" LTR 3011..3244 /note="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" misc_feature 3318..3906 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS 3424..3435 /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" CDS complement(3913..5343) /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /note="tdTomato" /note="mammalian codon-optimized" CDS complement(5353..5406) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS complement(5419..7068) /codon_start=1 /product="firefly luciferase" /note="luciferase" /note="synthetic luc2 version of the luciferase gene" LTR 7108..7376 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" promoter complement(7389..7600) /note="EF-1-alpha core promoter" /note="core promoter for human elongation factor EF-1-alpha" misc_feature 7645..7762 /note="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" misc_feature 8257..8490 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 8983..9108 /note="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" LTR 9152..9332 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1"ORIGIN 1 cagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 61 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 121 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta 181 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat 241 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc 301 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 361 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt 421 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 481 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 541 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 601 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 661 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 721 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 781 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 841 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 901 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 961 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 1021 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 1081 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 1141 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 1201 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 1261 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 1321 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 1381 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 1441 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 1501 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 1561 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 1621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 1681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 1741 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 1801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 1861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 1921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 1981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 2041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 2101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 2161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 2221 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 2281 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 2341 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 2401 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 2461 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 2521 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 2581 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt 2641 tgggccgtct ctgcaaaagt ctaggcctcc aaaaaagcct cctcactact tctggaatag 2701 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg 2761 cggagaatgg gcggaactgg gcggagttag gggcgggata gctagagcca gacatgataa 2821 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 2881 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttc 2941 ctctcactct ctgatattca tttctttgca agttataaat actgaataat aagatgacat 3001 gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta 3061 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga 3121 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac 3181 cagagagacc cagtacaagc aaaaagcaga tcttattttc gttgggagtg aattagccct 3241 tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt 3301 ggtcttaaag gtaccaggcg gggaggcggc ccaaagggag atccgactcg tctgagggcg 3361 aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc 3421 tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac 3481 acaggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg aattgtcagt 3541 gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt 3601 ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc 3661 ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac 3721 gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa 3781 agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt 3841 aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt aatccagagg 3901 ttgattgtcg acttacttgt acagctcgtc catgccgtac aggaacaggt ggtggcggcc 3961 ctcggagcgc tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag 4021 cttggtgtcc acgtagtagt agccgggcag ttgcacgggc ttcttggcca tgtagatggt 4081 cttgaactcc accaggtagt ggccgccgtc cttcagcttc agggcctggt ggatctcgcc 4141 cttcagcacg ccgtcgcggg ggtacaggcg ctcggtggag gcctcccagc ccatggtctt 4201 cttctgcatt acggggccgt cgggggggaa gttggtgccg cgcatcttca ccttgtagat 4261 cagcgtgccg tcctgcaggg aggagtcctg ggtcacggtc accagaccgc cgtcctcgaa 4321 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcttgt aatcggggat 4381 gtcggcgggg tgcttcacgt acgccttgga gccgtacatg aactgggggg acaggatgtc 4441 ccaggcgaag ggcagggggc cgcccttggt caccttcagc ttggcggtct gggtgccctc 4501 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca tggagccctc 4561 catgcgcacc ttgaagcgca tgaactcttt gatgacggcc atgttgttgt cctcggagga 4621 ggcggtgccg gagctgccgc taccggtgct gccggtgcca tgccccagga acaggtggtg 4681 gcggccctcg gagcgctcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat 4741 gtccagcttg gtgtccacgt agtagtagcc gggcagttgc acgggcttct tggccatgta 4801 gatggtcttg aactccacca ggtagtggcc gccgtccttc agcttcaggg cctggtggat 4861 ctcgcccttc agcacgccgt cgcgggggta caggcgctcg gtggaggcct cccagcccat 4921 ggtcttcttc tgcattacgg ggccgtcggg ggggaagttg gtgccgcgca tcttcacctt 4981 gtagatcagc gtgccgtcct gcagggagga gtcctgggtc acggtcacca gaccgccgtc 5041 ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct tcttgtaatc 5101 ggggatgtcg gcggggtgct tcacgtacgc cttggagccg tacatgaact ggggggacag 5161 gatgtcccag gcgaagggca gggggccgcc cttggtcacc ttcagcttgg cggtctgggt 5221 gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcatgga 5281 gccctccatg cgcaccttga agcgcatgaa ctctttgatg acctcctcgc ccttgctcac 5341 catggttccg gaagggccgg gattctcctc cacgtcaccg catgttagaa gacttcctct 5401 gccctcagcg gccgctaaca cggcgatctt gccgcccttc ttggccttaa tgagaatctc 5461 gcggatcttg cgggcgtcca acttgccggt cagtccttta ggcacctcgt ccacgaacac 5521 aacaccaccg cgcagcttct tggcggttgt aacctggctg gccacatagt ccacgatctc 5581 cttctcggtc atggttttac cgtgttccag cacgacgact gcggcgggca gctcgccggc 5641 atcgtcgtcg ggcaggccgg cgaccccggc gtcgaagatg ttggggtgtt gcagcaggat 5701 gctctccagt tcggctgggg ctacctggta gcccttgtat ttgatcaggc tcttcagccg 5761 gtccacgatg aagaagtgct cgtcctcgtc ccagtaggcg atgtcgccgc tgtgcagcca 5821 gccgtccttg tcgatgagag cgtttgtagc ctcggggttg ttaacgtagc cgctcatgat 5881 catggggcca cggacgcaca gctcgccgcg ctggttcaca cccagtgtct taccggtgtc 5941 caagtccacc accttagcct cgaagaaggg caccaccttg cctactgcgc caggcttgtc 6001 gtccccttcg ggggtgatca gaatggcgct ggttgtttct gtcaggccgt agccctggcg 6061 gatgcctggt aggtggaagc gtttggccac ggcctcacct acctccttgc tgagcggcgc 6121 cccgccgctg gcgatctcgt gcaagttgct taggtcgtac ttgtcgatga gagtgctctt 6181 agcgaagaag ctaaatagtg tgggcaccag cagggcagat tgaatcttat agtcttgcaa 6241 gctgcgcaag aatagctcct cctcgaagcg gtacatgagc acgacccgaa agccgcagat 6301 caagtagccc agcgtggtga acatgccgaa gccgtggtga aatggcacca cgctgaggat 6361 agcggtgtcg gggatgatct ggttgccgaa gatggggtcg cgggcatgac tgaatcggac 6421 acaagcggtg cggtgcggta gggctacgcc cttgggcaat ccggtactgc cactactgtt 6481 catgatcagg gcgatggttt tgtcccggtc gaagctctcg ggcacgaagt cgtactcgtt 6541 gaagccgggt ggcaaatggg aagtcacgaa ggtgtacatg ctttggaagc cctggtagtc 6601 ggtcttgcta tccatgatga tgatcttttg tatgatcggt agcttctttt gcacgttgag 6661 gatcttttgc agccctttct tgctcacgaa tacgacggtg ggctggctga tgcccatgct 6721 gttcagcagc tcgcgctcgt tgtagatgtc gttagctggg gccacagcca caccgatgaa 6781 cagggcaccc aacacgggca tgaagaactg caagctattc tcgctgcaca ccacgatccg 6841 atggtttgta ttcagcccat agcgcttcat agcttctgcc agccgaacgc tcatctcgaa 6901 gtactcggcg taggtaatgt ccacctcgat atgtgcgtcg gtaaaggcga tggtgccggg 6961 caccagggcg tagcgcttca tggctttgtg cagctgctcg ccggcggtcc cgtcttcgag 7021 tgggtagaat ggcgctgggc ccttcttaat gtttttggca tcttccatga attcgctagc 7081 tctagagtag gcgccggtca cagcttggat ctgtaacggc gcagaacaga aaacgaaaca 7141 aagacgtaga gttgagcaag cagggtcagg caaagcgtgg agagccggct gagtctaggt 7201 aggctccaag ggagcgccgg acaaaggccc ggtctcgacc tgagctttaa acttacctag 7261 acggcggacg cagttcagga ggcaccacag gcgggaggcg gcagaacgcg actcaaccgg 7321 cgtggatggc ggcctcaggt agggcggcgg gcgcgtgaag gagagatgcg agcccctcga 7381 agcttcagct gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact 7441 gcacttatat acggttctcc cccaccctcg ggaaaaaggc ggagccagta cacgacatca 7501 ctttcccagt ttaccccgcg ccaccttctc taggcacccg ttcaattgcc gacccctccc 7561 cccaacttct cggggactgt gggcgatgtg cgctctgccc actgacgggc accggagcga 7621 tcgcagatcc ttactagtat cgataaaatt ttgaattttt gtaatttgtt tttgtaattc 7681 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc 7741 aatcccccct tttcttttaa aagttaaccg ataccgtcga gatccgttca ctaatcgaat 7801 ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc 7861 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt 7921 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat 7981 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca 8041 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat 8101 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt 8161 gtgattccaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg 8221 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat 8281 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac 8341 agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac 8401 aataattgtc tggcctgtac cgtcagcgtc attgaggctg cgcccatagt gcttcctgct 8461 gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc 8521 actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat 8581 ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagt 8641 ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc 8701 ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt 8761 atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat 8821 ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc 8881 cctgcttgcc catactatat gttttaattt atattttttc tttccccctg gccttaaccg 8941 aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc 9001 tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc 9061 gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct ctggtttccc 9121 tttcgctttc aggtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag 9181 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc 9241 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc 9301 caggctcaga tctggtctaa ccagagagac ccgtttattg tatcgagcta ggcacttaaa 9361 tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca atccatgtca gacccgtctg 9421 ttgccttcct aataaggcac gatcgtacca ccttacttcc accaatcggc atgcacggtg 9481 ctttttctct ccttgtaagg catgttgcta actcatcgtt accatgttgc aagactacaa 9541 gagtattgca taagactaca cgcgt
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