pIB/V5-His

pIB/V5-His

载体基本信息

出品公司:	Ybscience
载体名称:	pIB/V5-His
质粒类型:	昆虫表达载体
克隆方法:	多克隆位点，限制性内切酶
启动子:	OplE2
载体大小:	3521 bp
5' 测序引物及序列:	OpIE2 Forward：CGCAACGATCTGGTAAACAC
3' 测序引物及序列:	5'd[CGCAACGATCTGGTAAACAC]3'
载体标签:	V5 Epitope Tag , His Tag (C-末端)
载体抗性:	氨苄青霉素
真核筛选标记:	Blasticidin
克隆菌株:	TOP10、DH5
宿主细胞（系）:	Sf9 、High Five
备注:	--
瞬表达/稳表达:	瞬表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

The InsectSelect BSD Kit features the pIB/V5-His vector for stable expression of your gene of interest in a variety of common insect cells. This vector has several features that facilitate 
expression, analysis, and detection of recombinant protein in insect cells:

• The OpIE2 promoter for constitutive expression
• The Blasticidin resistance gene for rapid selection of stably transfected cells in two weeks
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin

 

载体序列

LOCUS       pIB/V5-His	3521 bp 	DNA	SYN
DEFINITION  pIB/V5-His
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="pIB/V5-His"
                     /mol_type="other DNA"
     misc_feature    511..530
                     /label="OpIE2_fwd_primer"
     rep_origin      complement(987..1606)
                     /label="pBR322_origin"
     promoter        1980..2047
                     /label="EM7_promoter"
     gene            2048..2446
                     /label="blast (variant)"
                     /gene="blast (variant)"
     gene            2566..3426
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             2566..3426
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG
   61 TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT
  121 GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC
  181 GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC
  241 ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC
  301 GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT
  361 GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA
  421 GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCGCG TGACCGGACA CGAGGCGCCC
  481 GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG
  541 CATCTGTTCG AATTTAAAGC TTGGTACCGA GCTCGGATCC ACTAGTCCAG TGTGGTGGAA
  601 TTCTGCAGAT ATCCAGCACA GTGGCGGCCG CTCGAGTCTA GAGGGCCCGC GGTTCGAAGG
  661 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG CGTACCGGTC ATCATCACCA
  721 TCACCATTGA GTTTATCTGA CTAAATCTTA GTTTGTATTG TCATGTTTTA ATACAATATG
  781 TTATGTTTAA ATATGTTTTT AATAAATTTT ATAAAATAAT TTCAACTTTT ATTGTAACAA
  841 CATTGTCCAT TTACACACTC CTTTCAAGCG CGTGGGATCG ATGCTCACTC AAAGGCGGTA
  901 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
  961 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
 1021 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
 1081 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
 1141 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
 1201 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
 1261 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
 1321 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
 1381 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
 1441 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
 1501 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
 1561 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
 1621 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGCGAAA CACGCACGGC
 1681 GCGCGCACGC AGCTTAGCAC AAACGCGTCG TTGCACGCGC CCACCGCTAA CCGCAGGCCA
 1741 ATCGGTCGGC CGGCCTCATA TCCGCTCACC AGCCGCGTCC TATCGGGCGC GGCTTCCGCG
 1801 CCCATTTTGA ATAAATAAAC GATAACGCCG TTGGTGGCGT GAGGCATGTA AAAGGTTACA
 1861 TCATTATCTT GTTCGCCATC CGGTTGGTAT AAATAGACGT TCATGTTGGT TTTTGTTTCA
 1921 GTTGCAAGTT GGCTGCGGCG CGCGCAGCAC CTTTGCCGGG ATCTGCCGGG CTGCAGCACG
 1981 TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA
 2041 CTAAACCATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC
 2101 TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG
 2161 CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGCGCAGA
 2221 ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC
 2281 GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTTCTTCT
 2341 CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT
 2401 TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAAGCAC TTCGTGGCCG
 2461 AGGAGCAGGA CTGACACGTC CCGGGAGATC TGCATGTCTA CTAAACTCAC AAATTAGAGC
 2521 TTCAATTTAA TTATATCAGT TATTACCCAT TGAAAAAGGA AGAGTATGAG TATTCAACAT
 2581 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA
 2641 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC
 2701 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA
 2761 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG
 2821 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA
 2881 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA
 2941 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG
 3001 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG
 3061 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA
 3121 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA
 3181 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT
 3241 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA
 3301 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG
 3361 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT
 3421 TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT
 3481 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC T