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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
pAUR123
- 保质期：
6个月
- 供应商：
上海钰博生物科技有限公司
- 保存条件：
-20℃
- 规格：
1ug/5ug
pAUR123
| 编号 | 载体名称 |
| YB2700 | pAUR123 |
pAUR123载体基本信息
| 出品公司: | -- |
|---|---|
| 载体名称: | pAUR123 |
| 质粒类型: | 酵母表达载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | ADH1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6982bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 非病毒 |
pAUR123载体质粒图谱和多克隆位点信息
载体简介
pAUR vectors have a mutant AUR1-C gene derived from S.cerevisiae as a selective marker for yeast transformation system. pAUR123 is a protein expression vector and E. coli -yeast shuttle vector derived from pAUR112. It contains a constitutive promoter of ADH1 gene3 (Alcohol dehydrogenase1 gene) for protein expression in yeast cells. (Note: This vector is not suitable for high expression of protein.)
pAUR123载体序列
LOCUS AB012284 6982 bp DNA linear SYN 18-AUG-2007 DEFINITION Shuttle vector pAUR123 gene for Aur1-C, complete cds. ACCESSION AB012284 VERSION AB012284.1 GI:2992151 KEYWORDS Aur1-C variant; aureobasidin resistant protein. SOURCE Shuttle vector pAUR123 ORGANISM Shuttle vector pAUR123 other sequences; artificial sequences; vectors. REFERENCE 1 AUTHORS Hashida-Okado,T. TITLE Yeast-E. coli shuttle vector pAUR123 JOURNAL Published Only in Database (1998) REFERENCE 2 (bases 1 to 6982) AUTHORS Hashida-Okado,T. TITLE Direct Submission JOURNAL Submitted (18-MAR-1998) Takashi Hashida-Okado, Takara Shuzo Co.,Ltd., Biotechnology Research Laboratories; Seta 3-4-1, Otsu, Shiga 520-2193, Japan (E-mail:okadot@takara.co.jp, Tel:81-77-543-7298, Fax:81-77-543-2494) FEATURES Location/Qualifiers source 1..6982 /organism="Shuttle vector pAUR123" /mol_type="other DNA" /db_xref="taxon:75307" gene 4017..5222 /gene="AUR1-C" CDS 4017..5222 /gene="AUR1-C" /function="aureobasidin resistant" /note="variant" /codon_start=1 /transl_table=11 /product="Aur1-C" /protein_id="BAA25321.1" /db_xref="GI:2992152" /translation="MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSD WVHYIFLGSIMLFVFITNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALY FTSSYFPDDRRPPITVKVLPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHYGA PFVVAAILFVFGPPTVLQGYAFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDM HGSPGGLARIDKLLGINMYTTCFSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPL FIAYVCWLWWSTMYLTHHYFVDLMAGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSI EKYDISKSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATS ITSLGVKRA" ORIGIN 1 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 61 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 121 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 481 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 781 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga 841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 1021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 1081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 1141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 1201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 1261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 1321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 1381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 1441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 1501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 1561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 1621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 1681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 1741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 1801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 1861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 1921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 1981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 2041 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 2101 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 2161 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca 2221 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 2281 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg 2341 cagctgggcc attctcatga agaatatctt gaatttattg tcatattact agttggtgtg 2401 gaagtccata tatcggtgat caatatagtg gttgacatgc tggctagtca acattgagcc 2461 ttttgatcat gcaaatatat tacggtattt tacaatcaaa tatcaaactt aactattgac 2521 tttataactt atttaggtgg taacattctt ataaaaaaga aaaaaattac tgcaaaacag 2581 tactagcttt taacttgtat cctaggttat ctatgctgtc tcaccataga gaatattacc 2641 tatttcagaa tgtatgtcca tgattcgccg ggtaaataca tataatacac aaatctggct 2701 taataaagtc tataatatat ctcataaaga agtgctaaat tggctagtgc tatatatttt 2761 taagaaaatt tcttttgact aagtccatat cgactttgta aaagttcact ttagcataca 2821 tatattacac gagccagaaa ttgtaacttt tgcctaaaat cacaaattgc aaaatttaat 2881 tgcttgcaaa aggtcacatg cttataatca acttttttaa aaatttaaaa tactttttta 2941 ttttttattt ttaaacataa atgaaataat ttatttattg tttatgatta ccgaaacata 3001 aaacctgctc aagaaaaaga aactgttttg tccttggaaa aaaagcacta cctaggagcg 3061 gccaaaatgc cgaggctttc atagcttaaa ctctttacag aaaataggca ttatagatca 3121 gttcgagttt tcttattctt ccttccggtt ttatcgtcac agttttacag taaataagta 3181 tcacctctta gagttggcgg ttgtttgcaa gaccgagaaa aggctagcaa gaatcgggtc 3241 attgtagcgt atgcgcctgt gaacattctc ttcaacaagt ttgattccat tgcggtgaaa 3301 tggtaaaagt caaccccctg cgatgtatat tttcctgtac aatcaatcaa aaagccaaat 3361 gatttagcat tatctttaca tcttgttatt ttacagattt tatgtttaga tcttttatgc 3421 ttgcttttca aaaggcctgc aggcaagtgc acaaacaata cttaaataaa tactactcag 3481 taataaccta tttcttagca tttttgacga aatttgctat tttgttagag tcttttacac 3541 catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat 3601 caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctc gaatttcgag 3661 aaagtgccca tcagtgttca tatttgttac aaagtggttt tctgatttgg tactactgca 3721 gaggcgtatt ttttgcttca gttaccatag cgtaagaaca ctagcgactt ttgttcgtga 3781 accaacagag taggatttct actgctacat ctcttaggta gttggttagt ccgatcgctc 3841 acttttggtt gttgttaagt acttcataag tttatccttt tcctttttca cactgagcta 3901 ctttgggtat agcttttggc ccaaggatct ttgaattttc tccaaaagta ctttatttta 3961 tatcctacag gttgcggttt tcatatttta aaaagctttt aatcattcct ttgcgtatgg 4021 caaacccttt ttcgagatgg tttctatcag agagacctcc aaactgccat gtagccgatt 4081 tagaaacaag tttagatccc catcaaacgt tgttgaaggt gcaaaaatac aaacccgctt 4141 taagcgactg ggtgcattac atcttcttgg gatccatcat gctgtttgtg ttcattacta 4201 atcccgcacc ttggatcttc aagatccttt tttattgttt cttgggcact ttattcatca 4261 ttccagctac gtcacagttt ttcttcaatg ccttgcccat cctaacatgg gtggcgctgt 4321 atttcacttc atcgtacttt ccagatgacc gcaggcctcc tattactgtc aaagtgttac 4381 cagcggtgga aacaatttta tacggcgaca atttaagtga tattcttgca acatcgacga 4441 attccttttt ggacatttta gcatggttac cgtacggact atttcattat ggggccccat 4501 ttgtcgttgc tgccatctta ttcgtatttg gtccaccaac tgttttgcaa ggttatgctt 4561 ttgcatttgg ttatatgaac ctgtttggtg ttatcatgca aaatgtcttt ccagccgctc 4621 ccccatggta taaaattctc tatggattgc aatcagccaa ctatgatatg catggctcgc 4681 ctggtggatt agctagaatt gataagctac tcggtattaa tatgtatact acatgttttt 4741 caaattcctc cgtcattttc ggtgcttttc cttcactgca ttccgggtgt gctactatgg 4801 aagccctgtt tttctgttat tgttttccaa aattgaagcc cttgtttatt gcttatgttt 4861 gctggttatg gtggtcaact atgtatctga cacaccatta ttttgtagac cttatggcag 4921 gttctgtgct gtcatacgtt attttccagt acacaaagta cacacattta ccaattgtag 4981 atacatctct tttttgcaga tggtcataca cttcaattga gaaatacgat atatcaaaga 5041 gtgatccatt ggctgcagat tcaaacgata tcgaaagtgt ccctttgtcc aacttggaac 5101 ttgactttga tcttaatatg actgatgaac ccagtgtaag cccttcgtta tttgatggat 5161 ctacttctgt ttctcgttcg tccgccacgt ctataacgtc actaggtgta aagagggctt 5221 aatgagtatt ttatctgcaa ttacggatac ggttggtctt atgtagatac atataaatat 5281 atatcttttt ctttcttttt cttagtcagg attgtcgttt agcataatat acatgtagtt 5341 tatttaatca cataccactg attatcttta gaattttata aatttttgaa ataaatgggt 5401 ggcttttaat ggtgtctatg ttaagtgagg cttttagaat gctcttcctg ctttgtttat 5461 tatatgtgta tgaaagatat gtatgtattt acatgtgttt gtagcgtccc cagtcaaaac 5521 ctgtgcgcta tacctaaatg gattgataat cttcattcac taattctaaa atagacttct 5581 tccccaaaga acggtgtaac gatgaggctc tatccagctg cttatctaaa tcaactttaa 5641 cgatggatga tcttatgaca cggggatctt tctttaaagt tcttagaatt tcagactgta 5701 ccgcagctga tgaatcaaac agcattaaaa agtgatatgc tcgaaaatgt ttttcctggt 5761 ctttcttcat tattttagga agatacctta tgcccatggg tacaatgtcc ctcaccacac 5821 ctctgttttg aataatcagt ttcccgattg tggaagacaa ttcttttgct tccaactttg 5881 gcgcattgga gttggttatg cgaacaagtc cgatcagctc ataaagcatc ttagtgaaaa 5941 gggtggtttt gcgttattct ttcctctgga tcctctagct ccctaacatg taggtggcgg 6001 aggggagata tacaatagaa cagataccag acaagacata atgggctaaa caagactaca 6061 ccaattacac tgcctcattg atggtggtac ataacgaact aatactgtag ccctagactt 6121 gatagccatc atcatatcga agtttcacta ccctttttcc atttgccatc tattgaagta 6181 ataataggcg catgcaactt cttttctttt tttttctttt ctctctcccc cgttgttgtc 6241 tcaccatatc cgcaatgaca aaaaaatgat ggaagacact aaaggaaaaa attaacgaca 6301 aagacagcac caacagatgt cgttgttcca gagctgatga ggggtatctc gaagcacacg 6361 aaactttttc cttccttcat tcacgcacac tactctctaa tgagcaacgg tatacggcct 6421 tccttccagt tacttgaatt tgaaataaaa aaagtttgct gtcttgctat caagtataaa 6481 tagacctgca attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt 6541 gtttcttttt ctgcacaata tttcaagcta taccaagcat acaatcaact ggtacccggg 6601 tcgactcgag ctctagaggt taactaagcg aatttcttat gatttatgat ttttattatt 6661 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa 6721 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag 6781 catgaggtcg ctcttattga ccacatctct accggcatgc cgagcaaatg cctgcaaatc 6841 gctccccatt tcacccaatt gtagatatgc taactccagc aatgagttga tgaatctcgg 6901 tgtgtatttt atgtcctcag aggacaacac ctgttgtaat cgttcttcca cacggatcca 6961 cagcctagcc ttcagtcgag gc
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文献和实验相关实验
海洋微生物的培养基：Marine Agar (DSMZ Medium 123)
Composition per liter:Agar ……15.0gTryptone……10.0gPeptone ……5.0gYeast extract ……1.0gSynthetic seawater …… 1.0LpH 7.8 ± 0.2 at 25°CSynthetic Seawater Composition per liter:NaCl ……24.0gMgCl2·6H2O ……11.0gNa2SO4……4.0gCaCl2·6H2O……2.0gKCl……0.7gKBr……
【求助】Rhodamine 123 荧光强度和线粒体膜势能问题，求解！
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pAUR123
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