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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pcDNA3.1/Myc-His B
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pcDNA3.1/Myc-His B
pcDNA3.1/Myc-HisB载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pcDNA3.1/Myc-His B , pcDNA3.1/Myc-HisB |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5498 bp |
| 5' 测序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
| 3' 测序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
| 载体标签: | C-His, C-Myc |
| 载体抗性: | Ampicillin 氨苄 |
| 筛选标记: | Neomycin (G418) |
| 备注: | |
| 稳定性: | 瞬表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
pcDNA3.1/Myc-HisB载体质粒图谱和多克隆位点信息
pcDNA3.1/Myc-HisB载体简介
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
pcDNA3.1/Myc-HisB载体序列
LOCUS pcDNA3.1(+)/myc-His B 5498 bp DNA circular SYN 2-SEP-2003
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..5498
/invitrogen="127"
/vntifkey="98"
CDS 1046..1063
/invitrogen="1010000"
/vntifkey="4"
/label=6xHis
CDS complement(4502..5362)
/invitrogen="1020000"
/vntifkey="4"
/label=Amp(R)
polyA_site 1092..1316
/invitrogen="1870000"
/vntifkey="26"
/label=BGH\pA
primer complement(1086..1103)
/invitrogen="1910000"
/vntifkey="27"
/label=BGH\reverse\primer
promoter complement(5363..5461)
/invitrogen="2330000"
/vntifkey="30"
/label=bla\promoter
primer 769..789
/invitrogen="1930000"
/vntifkey="27"
/label=CMV\forward\primer
promoter 232..819
/invitrogen="2180000"
/vntifkey="29"
/label=CMV\promoter
CDS 1001..1030
/invitrogen="1090000"
/vntifkey="4"
/label=c-myc\epitope
rep_origin 1362..1790
/invitrogen="2500000"
/vntifkey="33"
/label=f1\origin
misc_feature 902..1003
/invitrogen="1510000"
/vntifkey="21"
/label=MCS
CDS 2201..2995
/invitrogen="1260000"
/vntifkey="4"
/label=Neo(R)
rep_origin complement(3684..4357)
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
promoter 1795..2165
/invitrogen="2290000"
/vntifkey="29"
/label=SV40\early\promoter
polyA_signal 3171..3301
/invitrogen="1840000"
/vntifkey="25"
/label=SV40\pA
primer 863..882
/invitrogen="2100000"
/vntifkey="27"
/label=T7\primer
promoter 863..879
/invitrogen="2370000"
/vntifkey="30"
/label=T7\promoter
BASE COUNT 1273 a 1428 c 1403 g 1394 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca
961 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaacaaaaac tcatctcaga
1021 agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt aaacccgctg
1081 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
1141 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
1201 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
1261 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
1321 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
1381 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
1441 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
1501 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga
1561 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
1621 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
1681 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc
1741 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
1801 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca
1861 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
1921 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
1981 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2041 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
2101 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
2161 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
2221 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
2281 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
2341 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
2401 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
2461 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
2521 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
2581 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
2641 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
2701 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
2761 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
2821 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
2881 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
2941 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
3001 actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
3061 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
3121 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
3181 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
3241 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
3301 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
3361 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
3421 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
3481 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
3541 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
3601 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
3661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
3721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
3781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
3841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
3901 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
3961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
4021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
4081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
4141 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
4201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
4261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
4321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
4381 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
4441 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
4501 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
4561 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
4621 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
4681 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
4741 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
4801 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
4861 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
4921 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
4981 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
5041 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
5101 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
5161 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
5221 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
5281 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
5341 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
5401 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
5461 ttccgcgcac atttccccga aaagtgccac ctgacgtc
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文献和实验相关实验
化，其他已知细胞松弛素 B还可抑制糖的输送，可以考虑细胞松弛素 B与细胞膜间的相互作用。此外还分离出数种具有类似结构和功能的物质，分别命名为细胞松弛素 A、 C、 D、 E、 F。
细胞色素b563 cytochrome b563 亦称细胞色素 b6 。存在于高等植物至藻类的叶绿体中，与类囊体膜结合紧密。含有原正铁血红素，氧化还原 （β带）和 434nm（γ带或 So－ ret带）。为发动光合磷酸化的环状电子传递系统的一个成分，可被光化系统Ⅰ所还原和氧化。
位于 A层以下、 C层以上的某种形式受成土因子的影响而形成的独特层次。一般来说， B层较 A层腐殖质含量少，多呈黄棕至红棕色。在湿润气候条件下有粘土、铁、铝等从 A层淋溶下来，在干燥气候条件下则有盐分从下层上升，两者都在 B层淀积，因此该层也称淀积层（ illuvial horizon）。
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pcDNA3.1/Myc-His B
¥800 - 1200
