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- 2025年07月14日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
plRES2-ZsGreen1
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | plRES2-ZsGreen1 |
| 质粒类型: | 哺乳动物表达载体 ； 双顺反子载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 启动子: | CMV |
| 载体大小: | 5.3kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 无 |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 备注: | ZsGreen1比EGFP亮度更高； IRES元件（内部核糖体结合位点） |
| 稳定性: | -- |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
IRES-containing bicistronic vectors allow the simultaneous expression of two proteins separately but from the same RNA transcript (1, 2). How Does an IRES Work? The IRES of the encephalomyocarditis virus (ECMV) permits the translation of two open reading frames from one messenger RNA. Although translation initiation of eukaryotic mRNAs occurs almost exclusively at the 5' cap, the IRES allows ribosomes to bind and initiate translation at a second, internal location. Thus, two proteins are expressed simultaneously from the same bicistronic mRNA transcript.Clontech's pIRES Vector contains two multiple cloning sites flanking the central IRES for you to clone and express two genes of interest.pIRES Vectors Containing Antibiotic Selection Markers The pIRES Selection Vectors pIRESpuro3, pIREShyg3, pIRESneo3, and pIRESbleo3 each contain a multiple cloning site located upstream of the IRES from which a selection marker is expressed. Because your gene of interest and a selection marker are translated from a single RNA, you can be certain that nearly 100% of the colonies that are resistant to puromycin, hygromycin, G418, and bleomycin also express your protein of interest. All IRES Selection Vectors contain a partially disabled IRES which reduces the efficiency of translation initiation for the selection marker relative to that of the cloned gene, and allows preferential selection of cells expressing high levels of your protein of interest (3). Bicistronic (IRES) Fluorescent Protein Expression Vectors Similarly, fluorescent protein-containing IRES vectors allow coexpression of your target protein and a fluorescent protein. Successfully transfected target cells are easily identified by fluorescence microscopy or flow cytometry. The are several options for different fluorescent proteins, which include mCherry, ZsGreen1, and tdTomato (pLVX-IRES Vectors) and AcGFP1, DsRed2, and DsRed-Express (pIRES2 Vectors). Features Translate your gene of interest and an antibiotic resistance marker or fluorescent protein from a single mRNA Quickly identify cells expressing your protein of interest at high levels Bicistronic expression allows faster, better stable clone selection Choice of vectors enables screening via antibiotic selection, fluorescence microscopy, or flow cytometry Applications Rapidly select stable clones that show high-level expression of your target protein Identify transfected cells by antibiotic selection, fluorescence microscopy, or flow cytometry References Jackson, R. J. et al. (1990) Trends Biochem. Sci. 15:477–483. Jang, S. K. et al. (1988) J. Virol. 62:2636–2643. Rees, S. et al. (1996) BioTechniques 20:102–110.
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文献和实验sunyuzhu10 包装质粒是pMD2.G pMDLg/pRRE pRSV-Rev 过表达载体是pLVX-IRES-ZsGreen1 这样的四个质粒能包装出慢病毒么？ 请有经验的战友帮忙看下 暴君 可以的 644414915 pMD2.G是VSVG吗 我现在正在包病毒，别人给了我这四个质粒，别的什么都没说，我不知道该怎么包装装染 谁有具体步骤 转染
相关专题 在研发转基因小鼠或基因嵌入报告基因模式小鼠的过程中，经常要用到报告基因。常用的报告基因包括：lacZ、Egfp 、zsGreen、EYFP、DsRed (DsRed2)、mRFP、mCherry、HuCD2、Thy1.1、HA tag、FLAG tag、Luciferase等。那什么时候选择哪种reporter呢? 做小鼠胚胎发育的研究人员一般喜欢用LacZ，也就是将LacZ置于一个启动子的调节之下。用LacZ可以进行胚胎全身
相关专题 在研发转基因小鼠或基因嵌入报告基因模式小鼠的过程中，经常要用到报告基因。常用的报告基因包括：lacZ、EGFP、zsGreen、EYFP、DsRed (DsRed2)、mRFP、mCherry、HuCD2、Thy1.1、HA tag、FLAG tag、Luciferase等。那什么时候选择哪种reporter呢? 做小鼠胚胎发育的研究人员一般喜欢用LacZ，也就是将LacZ置于一个启动子的调节之下。用LacZ可以进行胚胎全身染色
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