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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
6个月
- 英文名:
pEF1α-DsRed-Express2
- 库存:
大量
- 供应商:
钰博生物
- 规格:
1ug/5ug
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pEF1α-DsRed-Express2 |
| 质粒类型: | 哺乳动物细胞表达载体;荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶,多克隆位点 |
| 启动子: | EF1α |
| 载体大小: | 5395 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 无 |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 新霉素(Neomycin) |
| 克隆菌株: | DH5α, HB101 |
| 宿主细胞(系): | 常规细胞系,293、CV-1、CHO等 |
| 备注: | pEF1α-DsRed-Express2载体是哺乳动物细胞载体,DsRed-Express2蛋白; DsRed-Express2细胞毒性低,尤其适用于原代细胞和干细 胞的荧光标记; EF1α启动子是高表达启动子,对于那些使用CMV启动子表达效果不理想的细胞系具有良好效果。 。 |
| 稳定性: | 瞬表达 或 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
载体质粒图谱和多克隆位点信息


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文献和实验Noncytotoxic DsRed Derivatives for Whole-Cell Labeling
when expressed in the cytosol. This cytotoxicity stems from aggregation. To reduce aggregation, we engineered the surface of DsRed-Express to generate DsRed-Express2, a highly soluble tetrameric FP that is noncytotoxic in bacterial and mammalian cells. Directed
. We over-express the fluorescent protein DsRed during the larval and pupal stages of D. melanogaster development, and down-regulate DsRed in a line constitutively expressing this gene in the eyes. We then test the over-expression of Ultrabithorax (Ubx ) in B
Simultaneous Real-Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters
of increases in intracellular Ca2+ concentrations and the activation of protein kinase C (PKC) in response to the agonist activation of a Gαq/11 -coupled GPCR. Specifically, we will describe a confocal imaging approach to simultaneously measure oscillilations
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