pBI-CMV1

pBI-CMV1

pBI CMV1载体基本信息

出品公司:	Ybscience
载体名称:	pBI-CMV1, pBI CMV1
质粒类型:	哺乳动物细胞表达载体；双向表达载体
启动子:	miniCMV
拷贝数:	低拷贝
克隆方法:	多克隆位点，限制性内切酶
载体大小:	3114bp
5' 测序引物:	CMV-F
5' 测序引物序列:	5'-CGCAAATGGGCGGTAGGCGTG-3'
载体标签:	无
载体抗性:	Ampicillin
备注:	pBI-CMV1载体是双向表达载体，可以同时组成型的表达两个目的基因。
稳定性:	瞬表达
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

 

pBI CMV1载体质粒图谱和多克隆位点信息

pBI CMV1载体简介

 

pBI-CMV1 is a mammalian bidirectional expression vector that allows the constitutive expression of two proteins of interest. Protein expression is driven by one of two constitutively active, minimal human cytomegalovirus promoters, PminCMV1 and PminCMV2, located just upstream of two independent multiple cloning sites (MCS 1 and MCS 2, respectively). To allow propagation and selection in E. coli, the vector also contains a ColE1 origin of replication and an ampicillin resistance gene (Ampr).


pBI-CMV1 is designed to simultaneously and constitutively express two genes of interest. Each gene must be cloned into a different MCS, and contain both an initiation codon and a stop codon. The pBI-CMV1 vector can be transfected into mammalian cells using any standard transfection method.

 

pBI CMV1载体序列

LOCUS      pBI-CMV1           3114 bp ds-DNA    circular  SYN
DEFINITION  Mammalian bidirectional promoter vector for constitutively
            expressing two genes at similar levels.
ACCESSION   .
VERSION     .
KEYWORDS    pBI-CMV1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank|
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     The gene inserted into each MCS should contain start and stop
            codons.
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        67..370
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    602..663
                     /label="MCS 1"
                     /note="MCS 1"
                     /note="multiple cloning site 1"
     polyA_signal    777..858
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1040..1628)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1799..2659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2660..2764)
                     /gene="bla"
                     /note="AmpR promoter"
     polyA_signal    2797..2878
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    2986..3040
                     /label="MCS 2"
                     /note="MCS 2"
                     /note="multiple cloning site 2"
     promoter        complement(3069..3106)
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gggatcctct agtcagctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat
      661 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      841 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc
      901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2401 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2761 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg
     2821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     2941 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct
     3001 caggagatct gggcccccgc ggcatatgac cggtgaattc tccaggcgat ctgacggttc
     3061 actaaacgag ctctgcttat ataggcctcc caccgtacac gccacctcga cata