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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
6个月
- 英文名:
pGL4.10
- 库存:
大量
- 供应商:
钰博生物
- 规格:
1ug/5ug
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pGL4.10 |
| 质粒类型: | 哺乳细胞表达载体;信号通路报告载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | 无 |
| 克隆方法: | 多克隆位点,限制性内切酶 |
| 载体大小: | 4242bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay. |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
载体质粒图谱和多克隆位点信息



载体简介
The pGL4.10[luc2] Vector(a–d) encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.10[luc2] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice.
载体序列
LOCUS pGL4.10[luc2] 4242 bp DNA SYN
DEFINITION pGL4.10[luc2]
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4242
/organism="pGL4.10[luc2]"
/mol_type="other DNA"
CDS 100..1752
/label="ORF frame 1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
terminator complement(1793..1912)
/label="SV40_PA_terminator"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
misc_feature complement(1807..1826)
/label="EBV_rev_primer"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
rep_origin complement(2321..2940)
/label="pBR322_origin"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
CDS complement(3124..3984)
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 4089..4242
/label="TB"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
terminator 4089..4137
/label="synth_PA_terminator"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
terminator 4089..4242
/label="up_PA_terminator"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 4191..4210
/label="RVprimer3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC
61 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT
121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC
181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC
241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG
301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG
361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC
421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC
481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA
541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC
601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC
661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC
721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC
781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC
841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC
901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG
961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC
1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG
1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC
1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC
1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC
1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT
1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC
1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC
1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC
1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG
1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA
1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC
1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG
1741 ATCGCCGTGT AATAATTCTA GAGTCGGGGC GGCCGGCCGC TTCGAGCAGA CATGATAAGA
1801 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT
1861 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
1921 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
1981 AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGATAAGG ATCCGTCGAC CGATGCCCTT
2041 GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA TCGTCGCCGC
2101 ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG CGCTCTTCCG
2161 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
2221 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
2281 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
2341 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
2401 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
2461 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
2521 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
2581 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
2641 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
2701 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
2761 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
2821 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
2881 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
2941 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
3001 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
3061 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGCGGCCG CAAATGCTAA ACCACTGCAG
3121 TGGTTACCAG TGCTTGATCA GTGAGGCACC GATCTCAGCG ATCTGCCTAT TTCGTTCGTC
3181 CATAGTGGCC TGACTCCCCG TCGTGTAGAT CACTACGATT CGTGAGGGCT TACCATCAGG
3241 CCCCAGCGCA GCAATGATGC CGCGAGAGCC GCGTTCACCG GCCCCCGATT TGTCAGCAAT
3301 GAACCAGCCA GCAGGGAGGG CCGAGCGAAG AAGTGGTCCT GCTACTTTGT CCGCCTCCAT
3361 CCAGTCTATG AGCTGCTGTC GTGATGCTAG AGTAAGAAGT TCGCCAGTGA GTAGTTTCCG
3421 AAGAGTTGTG GCCATTGCTA CTGGCATCGT GGTATCACGC TCGTCGTTCG GTATGGCTTC
3481 GTTCAACTCT GGTTCCCAGC GGTCAAGCCG GGTCACATGA TCACCCATAT TATGAAGAAA
3541 TGCAGTCAGC TCCTTAGGGC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CGGTGTTGTC
3601 GCTCATGGTA ATGGCAGCAC TACACAATTC TCTTACCGTC ATGCCATCCG TAAGATGCTT
3661 TTCCGTGACC GGCGAGTACT CAACCAAGTC GTTTTGTGAG TAGTGTATAC GGCGACCAAG
3721 CTGCTCTTGC CCGGCGTCTA TACGGGACAA CACCGCGCCA CATAGCAGTA CTTTGAAAGT
3781 GCTCATCATC GGGAATCGTT CTTCGGGGCG GAAAGACTCA AGGATCTTGC CGCTATTGAG
3841 ATCCAGTTCG ATATAGCCCA CTCTTGCACC CAGTTGATCT TCAGCATCTT TTACTTTCAC
3901 CAGCGTTTCG GGGTGTGCAA AAACAGGCAA GCAAAATGCC GCAAAGAAGG GAATGAGTGC
3961 GACACGAAAA TGTTGGATGC TCATACTCGT CCTTTTTCAA TATTATTGAA GCATTTATCA
4021 GGGTTACTAG TACGTCTCTC AAGGATAAGT AAGTAATATT AAGGTACGGG AGGTATTGGA
4081 CAGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGAAT
4141 CGATAGTACT AACATACGCT CTCCATCAAA ACAAAACGAA ACAAAACAAA CTAGCAAAAT
4201 AGGCTGTCCC CAGTGCAAGT GCAGGTGCCA GAACATTTCT CT
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文献和实验,别人可以,自己确实不一定可以。 07 实验室的补助要涨了事实上,补助不降已经是学校和实验室对你最大的温柔。 08 等我搞科研发财了送外卖发财可能都比搞科研发财容易啊,发财了能怎么样,还不是做着用暴富的钱买进口试剂的梦。 09 我是老板带过的最有科研天赋的学生之一是的,每个人都会觉得他是其中之一,理论上,这样说没毛病。 10 师兄要发论文了,我帮过忙,能共一吧老板:共同通讯要不要啊,少年~~能坚持看完这篇推送的你,梦醒了吗?
科学界一直以来都有这样的传统:如果科学家找不到“小白鼠”,或者觉得让他人冒险很不道德,那么就会自己“献身”科学。自我实验就像一首英雄主义的史诗,不过也散发着一丝丝疯狂的气息。下面展示的这10个疯狂的自我实验,看来也是一个比一个癫狂。 10. 音速急刹车 第二次世界大战后,美国空军需要知道飞行员从超音速飞机中弹后能否逃过一劫,因为由音速到接近零的突然减速往往会导致死亡。这种速度的变化会让飞行员受到超过40G甚至50G的压力。(1G
Blunt PCR克隆乃利用传统T4连接酶方法,但载体和Topo平端的一样,含ccdB基因,所以同样可降低菌落背景,易于筛选。 表10 PCR克隆试剂盒的选择 Amplification Enzyme Application of Cloned PCR Product Ligation Method Product
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