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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pHook-3
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pHook-3
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pHOOK-3 |
| 质粒类型: | 哺乳动物表达载体；Capture-Tec系统；免疫磁珠 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 6199 bp |
| 5' 测序引物及序列: | CMV fwd 5’ CGCAAATGGGCGGTAGGCGTG3’ |
| 3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
| 载体标签: | IgK、HA tag (N-term)；2XMyc tag (N-term) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 博来霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳动物细胞 |
| 备注: | pHOOK3载体可以用于： 1. The effect of dominant, negative mutations of growth regulating proteins or toxic gene products 2. The acute effects of constitutive mutations 3. The effect of anti-sense RNA or ribozymes 4. Temporal events along a developmental or signaling pathway 5. Activation or inactivation of downstream genes using differential display 6. You may also use the pHook-3 System to isolate a homogeneous population of transfected cells for: a) Immunoblot analysis of proteins b) Isolation of DNA or RNA c) Flow Cytometry See pHOOK-1 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
pHook-3载体使用方法——免疫磁珠法
载体序列
LOCUS Exported 6199 bp ds-DNA circular SYN 13-AUG-2016
KEYWORDS pHOOK-3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6199)
AUTHORS Biofeng Lab.
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6199
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 29..358
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 209..344
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 787..834
/note="EM7 promoter"
/note="synthetic bacterial promoter "
promoter 826..844
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 854..1228
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 1292..1426
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1590..2178)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 2263..2566
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 2567..2770
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 2905..2923
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 3261..3309
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter 4079..4341
/note="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
CDS 4366..4428
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/note="Ig-kappa leader"
/translation="METDTLLLWVLLLWVPGSTGD"
CDS 4429..4455
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
CDS 5194..5223
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 5239..5268
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
promoter complement(5460..5478)
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
polyA_signal 5504..5728
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 5774..3
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 attatggtga ccggatccgc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
61 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
121 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
181 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
241 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
301 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
361 gctggctcga tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
421 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
481 taagtttaga gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
541 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
601 cgttttctgt tctgcgccgt tacaacttca aggtatgcgc tgggacctgg caggcggcat
661 ctgggacccc taggaagggc ttgggggtcc tcgtgcccaa ggcagggaac atagtggtcc
721 caggaagggg agcagaggca tcagggtgtc cactttgtct ccgcagctcc tgagcctgca
781 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgactcacta
841 taggagggcc accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
901 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
961 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
1021 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
1081 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
1141 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
1201 gcacttcgtg gccgaggagc aggactgacc gacgccgacc aacaccgccg gtccgacggc
1261 ggcccacggg tcccaggggg gtcgacctcg aaacttgttt attgcagctt ataatggtta
1321 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
1381 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccctc ggagatcgat
1441 ctgggcccat gcggccggcc gcggatcgat gctcactcaa aggcggtaat acggttatcc
1501 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
1561 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
1621 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
1681 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
1741 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
1801 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
1861 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
1921 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
1981 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
2041 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
2101 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
2161 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
2221 aacgaaaact cacgttaagg gattttggtc atggctagag tccgttacat aacttacggt
2281 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
2341 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
2401 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
2461 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
2521 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
2581 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
2641 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
2701 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
2761 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
2821 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
2881 ggacctgcag cacgtgttga caattaatac gactcactat agggagaccc aagcttggta
2941 ccagatcttt taaagctagc tctagagtac cgagctcgga tcccccgggc cagtgtgctg
3001 gaagtaccga gctcgatcgt acccagcttg gaggttgcag ggtcgatgcg acgcaatcgt
3061 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
3121 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
3181 gagggcaaag gaatagggga gatgggggag gctaactgaa acacggaagg agacaatacc
3241 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
3301 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
3361 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
3421 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg
3481 gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat
3541 tattttgggc gttgcgtggg gtctggtcca cgactggact gagcagacag acccatggtt
3601 tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg
3661 gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgc ccagtggtcg
3721 accaattctc atgtttgaca gcttatcatc gcagatcctg agcttgtatg gtgcactctc
3781 agtacaatct gctctgctgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
3841 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
3901 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
3961 agatatacgc gtatctgagg ggactagggt gtgtttaggc gcccagcggg gcttcggttg
4021 tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa
4081 tgtagtctta tgcaatacac ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
4141 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
4201 gtgccttatt aggaaggcaa cagacaggtc tgacatggat tggacgaacc actgaattcc
4261 gcattgcaga gataattgta tttaagtgcc tagctcgata caataaacgc catttgacca
4321 ttcaccacat tggtgtgcac ctccaagctg ggtacgatat ccaccatgga gacagacaca
4381 ctcctgctat gggtactgct gctctgggtt ccaggttcca ctggtgacta tccatatgat
4441 gttccagatt atgctggggc ccagccggcc atggccgagg tcaagctgca ggagtcaggg
4501 ggaggcttag tgcagcctgg agggtcccgg aaactctcct gtgcagcctc tggattcact
4561 ttcagtagct ttggaatgca ctgggttcgt caggctccag agaaggggct ggagtgggtc
4621 gcatatatta gtagtggcag tagtaccatc tactatgcag acacagtgaa gggacgattc
4681 accatctcca gagacaatcc caagaacacc ctgttcctgc aaatgaccag tctaaggtct
4741 gaggacacgg ncatgtatta ctgtgcaaga gattacgggg cttattgggg ccaagggacc
4801 acggncaccg tctcctcagg tggaggcggc tcaggcggag gtggctctgg cggtggcgga
4861 tcggacattg agctcaccca gtctccagca atcatgtctg catctccagg ggagagggtc
4921 accatgacct gcagtgccag ttcaagtgta aggtacatga actggttcca acagaagtca
4981 ggcacctccc ccaaaagatg gatttatgac acatccaaac tgtcttctgg agtccctgct
5041 cgcttcagtg gcagtgggtc tgggacctct tactctctca caatcagcag catggaggct
5101 gaagatgctg ccacttacta ctgccagcag tggagtagta acccactcac gttcggtgct
5161 gggaccaagc tggagctgaa acgggcggcc gcagaacaaa aactcatctc agaagaggat
5221 ctgaatgggg ccgtcgacga acaaaaactc atctcagaag aggatctgaa tgctgtgggc
5281 caggacacgc aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc
5341 tcagccatcc tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgctt
5401 tggcagaaga agccacgtta ggcggccgct cgagcatgca tctagctaga gggccctatt
5461 ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
5521 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
5581 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
5641 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
5701 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
5761 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
5821 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
5881 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
5941 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
6001 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
6061 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
6121 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
6181 taacaaaaat ttaacgcga
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pHook-3
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