pCAG-T7-TALEN(Sangamo)-Destina

pCAG-T7-TALEN(Sangamo)-Destination

载体基本信息

质粒名称:	pCAG-T7-TALEN(Sangamo)-Destination
出品公司:	Ybscience
存储实验室:	Pawel Pelczar
用途:	采用同源FokI域
载体骨架基本信息
载体名称:	pDIRECT
载体出品公司:	CLONTECH
载体抗性:	氨苄霉素
空载体大小:	4810 bp
载体类型:	哺乳细胞表达
高拷贝/低拷贝:	高拷贝
生长条件:	37 ℃
插入基因信息
启动子	CAG
标签/融合蛋白:	FokI nuclease（C端）
克隆方法:	限制性内切酶
5' 克隆位点:
3' 克隆位点:
5' 测序引物	TAL_F1 (ttggcgtcggcaaacagtgg)
3' 测序引物	TAL_R2 (ggcgacgaggtggtcgttg
文献引用方法
材料和方法部分:	pCAG-T7-TALEN(Sangamo)-Destination was a gift from Pawel Pelczar (Addgene plasmid # 37184)
参考文献部分:	Mouse genome engineering using designer nucleases. Hermann M, Cermak T, Voytas DF, Pelczar P. J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930. 10.3791/50930 PubMed 24747757

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       pCAG-T7-TALEN(Sangamo)-Destination6441 bp DNASYNDEFINITION  pCAG-T7-TALEN(Sangamo)-DestinationACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..6441                     /organism="pCAG-T7-TALEN(Sangamo)-Destination"                     /mol_type="other DNA"     promoter        86..115                     /label="lac_promoter"     misc_feature    129..151                     /label="M13_pUC_rev_primer"     promoter        150..168                     /label="M13_reverse_primer"     misc_feature    264..551                     /label="CAG_enhancer"     CDS             complement(983..1714)                     /label="ORF frame 3"     CDS             complement(1024..1527)                     /label="ORF frame 1"     promoter        1864..1882                     /label="T7_promoter"     CDS             1893..2501                     /label="ORF frame 3"     misc_feature    1902..1922                     /label="SV40 NLS"     misc_feature    1908..1922                     /label="NLS"     misc_feature    complement(2357..2512)                     /label="lacZ_a"     misc_feature    2483..2505                     /label="M13_pUC_fwd_primer"     promoter        2498..2514                     /label="M13_forward20_primer"     promoter        2524..2542                     /label="T7_promoter"     promoter        complement(2589..2608)                     /label="T3_promoter"     promoter        complement(2625..2643)                     /label="M13_reverse_primer"     misc_feature    complement(2642..2664)                     /label="M13_pUC_rev_primer"     promoter        complement(2678..2707)                     /label="lac_promoter"     CDS             2722..3519                     /label="ORF frame 1"     misc_feature    complement(3538..3555)                     /label="BGH_rev_primer"     terminator      3541..3768                     /label="bGH_PA_terminator"     CDS             3742..4215                     /label="ORF frame 1"     promoter        complement(3840..3856)                     /label="M13_forward20_primer"     misc_feature    complement(3849..3871)                     /label="M13_pUC_fwd_primer"     misc_feature    3849..3997                     /label="lacZ_a"     rep_origin      4015..4321                     /label="f1_origin"     promoter        4514..4542                     /label="AmpR_promoter"     gene            4584..5444                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             4584..5444                     /label="ORF frame 3"     rep_origin      5599..6218                     /label="pBR322_origin"ORIGIN    1 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT   61 AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG  121 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAG GCGCGCCGGA  181 TTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA  241 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC  301 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG  361 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC  421 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG  481 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG  541 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA  601 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG  661 CGATGGGGGC GGGGGGGGGG GGGGGGCGCG CGCCAGGCGG GGCGGGGCGG GGCGAGGGGC  721 GGGGCGGGGC GAGGCGGAGA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT  781 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG  841 CGGGAGTCGC TGCGTTGCCT TCGCCCCGTG CCCCGCTCCG CGCCGCCTCG CGCCGCCCGC  901 CCCGGCTCTG ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC CCTTCTCCTC  961 CGGGCTGTAA TTAGCGCTTG GTTTAATGAC GGCTCGTTTC TTTTCTGTGG CTGCGTGAAA 1021 GCCTTAAAGG GCTCCGGGAG GGCCCTTTGT GCGGGGGGGA GCGGCTCGGG GGGTGCGTGC 1081 GTGTGTGTGT GCGTGGGGAG CGCCGCGTGC GGCCCGCGCT GCCCGGCGGC TGTGAGCGCT 1141 GCGGGCGCGG CGCGGGGCTT TGTGCGCTCC GCGTGTGCGC GAGGGGAGCG CGGCCGGGGG 1201 CGGTGCCCCG CGGTGCGGGG GGGCTGCGAG GGGAACAAAG GCTGCGTGCG GGGTGTGTGC 1261 GTGGGGGGGT GAGCAGGGGG TGTGGGCGCG GCGGTCGGGC TGTAACCCCC CCCTGCACCC 1321 CCCTCCCCGA GTTGCTGAGC ACGGCCCGGC TTCGGGTGCG GGGCTCCGTG CGGGGCGTGG 1381 CGCGGGGCTC GCCGTGCCGG GCGGGGGGTG GCGGCAGGTG GGGGTGCCGG GCGGGGCGGG 1441 GCCGCCTCGG GCCGGGGAGG GCTCGGGGGA GGGGCGCGGC GGCCCCGGAG CGCCGGCGGC 1501 TGTCGAGGCG CGGCGAGCCG CAGCCATTGC CTTTTATGGT AATCGTGCGA GAGGGCGCAG 1561 GGACTTCCTT TGTCCCAAAT CTGGCGGAGC CGAAATCTGG GAGGCGCCGC CGCACCCCCT 1621 CTAGCGGGCG CGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT 1681 TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCATCTCCAG CCTCGGGGCT GCCGCAGGGG 1741 GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC 1801 GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GATCCTTAAT 1861 TAATAATACG ACTCACTATA GGGGCCGCCA CCATGAGATC TCCTAAGAAA AAGAGGAAGG 1921 TGCAGGTGGA TCTACGCACG CTCGGCTACA GTCAGCAGCA GCAAGAGAAG ATCAAACCGA 1981 AGGTGCGTTC GACAGTGGCG CAGCACCACG AGGCACTGGT GGGCCATGGG TTTACACACG 2041 CGCACATCGT TGCGCTCAGC CAACACCCGG CAGCGTTAGG GACCGTCGCT GTCACGTATC 2101 AGCACATAAT CACGGCGTTG CCAGAGGCGA CACACGAAGA CATCGTTGGC GTCGGCAAAC 2161 AGTGGTCCGG CGCACGCGCC CTGGAGGCCT TGCTCACGGA TGCGGGGGAG TTGAGAGGTC 2221 CGCCGTTACA GTTGGACACA GGCCAACTTG TGAAGATTGC AAAACGTGGC GGCGTGACCG 2281 CAATGGAGGC AGTGCATGCA TCGCGCAATG CACTGACGGG TGCCCCCCTG GAGACGGGCG 2341 CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG 2401 GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA 2461 AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC 2521 GCGTAATACG ACTCACTATA GGGCGAATTG GGTACCGGGC CCCCCCTCGA GGTCCTCCAG 2581 CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT 2641 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA 2701 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT 2761 GCCCGCTTTC CACCGGTCGT CTCCAACGAC CACCTCGTCG CCTTGGCCTG CCTCGGCGGA 2821 CGTCCTGCCA TGGATGCAGT GAAAAAGGGA TTGCCGCACG CGCCGGAATT GATCAGAAGA 2881 GTCAATCGCC GTATTGGCGA ACGCACGTCC CATCGCGTTg cggGATCcca gctagtgaaa 2941 tctgaattgg aaGAGAAGAA ATCTGAACTT AGACATAAAT TGAAATATGT GCCACATGAA 3001 TATATTGAAT TGATTGAAAT CGCAAGAAAT TCAACTCAGG ATAGAATCCT TGAAATGAAG 3061 GTGATGGAGT TCTTTATGAA GGTTTATGGT TATCGTGGTA AACATTTGGG TGGATCAAGG 3121 AAACCAGACG GAGCAATTTA TACTGTCGGA TCTCCTATTG ATTACGGTGT GATCGTTGAT 3181 ACTAAGGCAT ATTCAGGAGG TTATAATCTT CCAATTGGTC AAGCAGATGA AATGCAAAGA 3241 TATGTCGAAG AGAATCAAAC AAGAAACAAG CATATCAACC CTAATGAATG GTGGAAAGTC 3301 TATCCATCTT CAGTAACAGA ATTTAAGTTC TTGTTTGTGA GTGGTCATTT CAAAGGAAAC 3361 TACAAAGCTC AGCTTACAAG ATTGAATCAT ATCACTAATT GTAATGGAGC TGTTCTTAGT 3421 GTAGAAGAGC TTTTGATTGG TGGAGAAATG ATTAAAGCTG GTACATTGAC ACTTGAGGAA 3481 GTGAGAAGGA AATTTAATAA CGGTGAGATA AACTTTTAAT AGGAGCTCGC TGATCAGCCT 3541 CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA 3601 CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT 3661 GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG 3721 ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG 3781 AAAGAACCAG CTGGGGCTCG AGATCCACTA GTTCTAGCCT CGAGGCTAGA GCGGCCGCCA 3841 CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC 3901 CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC 3961 CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA 4021 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 4081 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 4141 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 4201 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 4261 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 4321 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 4381 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA 4441 ACGCTTACAA TTTAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4501 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4561 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4621 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4681 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4741 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4801 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 4861 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 4921 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 4981 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 5041 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 5101 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5161 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 5221 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 5281 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5341 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5401 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5461 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5521 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5581 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5641 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5701 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5761 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 5821 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 5881 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 5941 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 6001 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 6061 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 6121 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 6181 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 6241 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6301 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6361 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6421 GGCCGATTCA TTAATGCAGC T